Construct: ORF TRCN0000489010

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020125.1_s317c1
DNA Barcode:
CATAGGTAGGCGTCCACATGACTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
AK5 (26289)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489010
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26289 AK5 adenylate kinase 5 XM_017001010.2 38.4% 38.4% 1_945del
2 human 26289 AK5 adenylate kinase 5 XM_017001011.2 38.4% 38.4% 1_945del
3 human 26289 AK5 adenylate kinase 5 NM_012093.4 36.7% 36.7% 1_1017del
4 human 26289 AK5 adenylate kinase 5 XM_006710572.3 36.7% 36.7% 1_1017del
5 human 26289 AK5 adenylate kinase 5 XM_017001008.2 36.7% 36.7% 1_1017del
6 human 26289 AK5 adenylate kinase 5 XM_017001009.1 36.7% 36.7% 1_1017del
7 human 26289 AK5 adenylate kinase 5 XM_005270739.5 36.6% 36.6% 1_1023del
8 human 26289 AK5 adenylate kinase 5 NM_174858.3 35% 35% 1_1095del
9 mouse 229949 Ak5 adenylate kinase 5 NM_001081277.1 30.4% 30.4% (many diffs)
10 mouse 229949 Ak5 adenylate kinase 5 XM_011240115.2 29.3% 27.3% (many diffs)
11 mouse 229949 Ak5 adenylate kinase 5 XM_006501440.3 27% 27% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
663
ORF length:
591
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgggaggt ttcatggaag atttgagaaa gtgtaaaatt attttcataa
121ttggtggtcc tggctctggc aaaggcacac agtgtgaaaa gctggtggaa aaatatggat
181ttacacatct ctcaactggc gagctcctgc gtgaggaact ggcatcagaa tctgaaagaa
241gcaaattgat cagagacatt atggaacgtg gagacctggt gccctcaggc atcgttttgg
301agctcctgaa ggaggccatg gtggccagcc tcggggacac caggggcttc cTGATTGACG
361GCTATCCTCG GGAGGTGAAG CAAGGGGAAG AGTTCGGACG CAGGATTGGA GACCCACAGT
421TGGTGATCTG TATGGACTGC TCGGCAGACA CCATGACCAA CCGCCTTCTC CAAAGGAGCC
481GGAGCAGCCT GCCTGTGGAC GACACCACCA AGACCATCGC CAAGCGCCTA GAAGCCTACT
541ACCGAGCGTC CATCCCCGTG ATCGCCTACT ACGAGACAAA AACACAGCTA CACAAGATAA
601ATGCAGAGGG AACACCAGAG GACGTTTTTC TTCAACTCTG CACAGCTATT GACTCTATTT
661TCTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
721TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
781CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATAGGTAG GCGTCCACAT GACTAACGCG
841TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)