Transcript: Human XM_017001010.2

PREDICTED: Homo sapiens adenylate kinase 5 (AK5), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AK5 (26289)
Length:
4149
CDS:
1318..2856

Additional Resources:

NCBI RefSeq record:
XM_017001010.2
NBCI Gene record:
AK5 (26289)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001010.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196485 GTGCTATCAATCAGTTGTAAA pLKO.1 3925 3UTR 100% 13.200 18.480 N AK5 n/a
2 TRCN0000220633 CGATATGGATTCCAATACATT pLKO.1 1630 CDS 100% 5.625 7.875 N AK5 n/a
3 TRCN0000335948 CGATATGGATTCCAATACATT pLKO_005 1630 CDS 100% 5.625 7.875 N AK5 n/a
4 TRCN0000194731 CGGAGATCCTTTCTAAGAAAT pLKO.1 1465 CDS 100% 13.200 10.560 N AK5 n/a
5 TRCN0000196669 GTGATGCATTTCAGCAATTAT pLKO.1 3605 3UTR 100% 15.000 10.500 N AK5 n/a
6 TRCN0000220635 CCTTGATCCTTCGATGATATT pLKO.1 2160 CDS 100% 13.200 9.240 N AK5 n/a
7 TRCN0000335949 CCTTGATCCTTCGATGATATT pLKO_005 2160 CDS 100% 13.200 9.240 N AK5 n/a
8 TRCN0000220632 GCACAGCTATTGACTCTATTT pLKO.1 2831 CDS 100% 13.200 9.240 N AK5 n/a
9 TRCN0000335950 GCACAGCTATTGACTCTATTT pLKO_005 2831 CDS 100% 13.200 9.240 N AK5 n/a
10 TRCN0000196484 GCATGAGATATTTGCTATTTC pLKO.1 3659 3UTR 100% 13.200 9.240 N AK5 n/a
11 TRCN0000381362 TCAGGGTGATGACCAGTTAAA pLKO_005 2223 CDS 100% 13.200 9.240 N AK5 n/a
12 TRCN0000196382 GCATTGTTATTGATGGATTTC pLKO.1 1808 CDS 100% 10.800 7.560 N AK5 n/a
13 TRCN0000195147 CAAGGTGAAATGGGATACATT pLKO.1 1395 CDS 100% 5.625 3.938 N AK5 n/a
14 TRCN0000220631 CCTCTCATTAAGCATCCCTAA pLKO.1 3220 3UTR 100% 4.050 2.835 N AK5 n/a
15 TRCN0000335867 GGCAGTTGACAACAAGTTATT pLKO_005 2106 CDS 100% 13.200 7.920 N AK5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001010.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488605 TATCTCCTCTGCAGGTCTTGTGGC pLX_317 18.9% 91.1% 91.1% V5 (not translated due to prior stop codon) 0_1ins78;168_169ins72 n/a
2 TRCN0000491369 GCCTTTCTAGTGGCGGGGCCTAGA pLX_317 18.8% 91% 90.9% V5 0_1ins78;168_169ins72;1536_1537insG n/a
3 TRCN0000489010 CATAGGTAGGCGTCCACATGACTA pLX_317 52.4% 38.4% 38.4% V5 (not translated due to prior stop codon) 1_945del n/a
4 TRCN0000491546 AGGCATTCTGCGACTCAAGTTTGA pLX_317 60.9% 38.4% 38.4% V5 (not translated due to prior stop codon) 1_945del n/a
5 TRCN0000488181 GGGGGCGCCCTGAACCATACCTAT pLX_317 52.4% 38.4% 38.4% V5 1_945del;1536_1537insG n/a
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