Construct: ORF TRCN0000489018

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019566.1_s317c1
DNA Barcode:
TGACCTCGTGACCAGCTTGGGGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
INPP5K (51763)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489018
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51763 INPP5K inositol polyphosphate-5-ph... NM_016532.4 100% 100%
2 human 51763 INPP5K inositol polyphosphate-5-ph... NM_001135642.2 83% 83% 0_1ins228
3 human 51763 INPP5K inositol polyphosphate-5-ph... NM_130766.3 83% 83% 0_1ins228
4 human 51763 INPP5K inositol polyphosphate-5-ph... XM_005256683.2 83% 83% 0_1ins228
5 human 51763 INPP5K inositol polyphosphate-5-ph... XM_011523934.1 83% 83% 0_1ins228
6 human 51763 INPP5K inositol polyphosphate-5-ph... XM_017024756.1 83% 83% 0_1ins228
7 human 51763 INPP5K inositol polyphosphate-5-ph... XM_024450802.1 83% 83% 0_1ins228
8 human 51763 INPP5K inositol polyphosphate-5-ph... XM_005256685.1 79.4% 79.4% 0_1ins276
9 human 51763 INPP5K inositol polyphosphate-5-ph... XM_005256686.2 79.4% 79.4% 0_1ins276
10 human 51763 INPP5K inositol polyphosphate-5-ph... XM_017024757.2 79.4% 79.4% 0_1ins276
11 human 51763 INPP5K inositol polyphosphate-5-ph... XM_011523936.2 68.6% 60.9% 0_1ins415;140_149delGTGAGCCTGG
12 human 51763 INPP5K inositol polyphosphate-5-ph... XM_017024758.2 68.6% 60.9% 0_1ins415;140_149delGTGAGCCTGG
13 human 51763 INPP5K inositol polyphosphate-5-ph... XM_017024759.1 68.6% 60.9% 0_1ins415;140_149delGTGAGCCTGG
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
1437
ORF length:
1344
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcaccgaa ttcacggcgg ccatgagctc gcggaagctg agcgggccga
121aaggcaggag gctcagcata cacgtcgtga cttggaacgt ggcttcggca gcgccccctc
181tagatctcag tgacctgctt cagctgaaca accggaacct caatcttgac atatatgtta
241ttggtttgca ggaattgaac tctgggatca taagcctcct ttccgatgct gcctttaatg
301actcgtggag cagtttcctc atggatgtgc tttcccctct gagcttcatc aaggtctccc
361atgtccgtat gcaggggatc ctcttactgg tctttgccaa gtatcagcat ttgccctata
421tccagattct gtctactaaa tccaccccca ctggcctgtt tgggtactgg gggaacaaag
481gtggagtcaa catctgcctg aagctttatg gctactatgt cagcatcatc aactgccacc
541tgcctcccca catttccaac aattaccagc ggctggagca ctttgaccgg atcctggaga
601tgcagaattg tgaggggcga gacatcccaa acatcctgga ccacgacctc attatctggt
661ttggagacat gaactttcgg atcgaggact ttgggttgca ctttgttcgg gaatccatta
721aaaatcggtg ctacggtggc ctgtgggaga aggaccagct cagcattgcc aagaaacatg
781acccgctgct ccgggagttc caggagggcc gcctactctt cccgcccacc tacaagtttg
841ataggaactc caacgactat gacaccagtg agaaaaaacg caagcctgca tggaccgatc
901gcatcctgtg gaggctgaag cggcagccct gtgctggccc cgacactccc ataccgccgg
961cgtcacactt ctccttgtct ctgaggggct acagcagcca catgacgtac ggcatcagcg
1021accacaagcc tgtctccggc acgttcgact tggagctgaa gccattggtg tctgctccgc
1081tgatcgtcct gatgcccgag gaccTGTGGA CCGTGGAAAA TGACATGATG GTCAGCTACT
1141CTTCAACCTC GGACTTCCCC AGCAGCCCGT GGGACTGGAT TGGACTGTAC AAGGTGGGGC
1201TGCGGGACGT TAATGACTAC GTGTCCTATG CCTGGGTCGG GGACAGCAAG GTCTCCTGCA
1261GCGACAACCT GAACCAGGTT TACATCGACA TCAGCAATAT CCCTACCACT GAAGATGAGT
1321TTCTCCTCTG TTACTACAGC AACAGTCTGC GTTCTGTGGT GGGGATAAGC AGACCCTTCC
1381AGATCCCGCC TGGCTCCTTG AGGGAGGACC CACTGGGTGA AGCACAGCCA CAGATCAAGG
1441GTGGGCGCGC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1501ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1561CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGA CCTCGTGACC AGCTTGGGGA
1621TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

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