Construct: ORF TRCN0000489033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021005.1_s317c1
- DNA Barcode:
- AAACGATTACCACGTACTCTGTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM2 (2912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2912 | GRM2 | glutamate metabotropic rece... | NM_000839.5 | 100% | 100% | |
| 2 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_017006271.1 | 100% | 100% | |
| 3 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533635.1 | 96.3% | 96.3% | 1287_1288ins96 |
| 4 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533636.1 | 79.1% | 79.1% | 445_446ins546 |
| 5 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533637.1 | 77.7% | 77.7% | 0_1ins582 |
| 6 | human | 2912 | GRM2 | glutamate metabotropic rece... | NM_001349116.2 | 56.6% | 56.6% | 0_1ins1134 |
| 7 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533642.1 | 53.8% | 53.8% | 1410_1411ins1206 |
| 8 | human | 2912 | GRM2 | glutamate metabotropic rece... | NM_001349117.2 | 52.6% | 51.6% | (many diffs) |
| 9 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_024453490.1 | 49.3% | 49.1% | (many diffs) |
| 10 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533643.1 | 49% | 42.6% | (many diffs) |
| 11 | human | 2912 | GRM2 | glutamate metabotropic rece... | NR_146059.2 | 45.1% | (many diffs) | |
| 12 | mouse | 108068 | Grm2 | glutamate receptor, metabot... | NM_001160353.1 | 89.9% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2688
- ORF length:
- 2616
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggatcg ctgcttgcgc tcctggcact gctgctgctg tggggtgctg 121 tggctgaggg cccagccaag aaggtgctga ccctggaggg agacttggtg ctgggtgggc 181 tgttcccagt gcaccagaag ggcggcccag cagaggactg tggtcctgtc aatgagcacc 241 gtggcatcca gcgcctggag gccatgcttt ttgcactgga ccgcatcaac cgtgacccgc 301 acctgctgcc tggcgtgcgc ctgggtgcac acatcctcga cagttgctcc aaggacacac 361 atgcgctgga gcaggcactg gactttgtgc gtgcctcact cagccgtggt gctgatggct 421 cacgccacat ctgccccgac ggctcttatg cgacccatgg tgatgctccc actgccatca 481 ctggtgttat tggcggttcc tacagtgatg tctccatcca ggtggccaac ctcttgaggc 541 tatttcagat cccacagatt agctacgcct ctaccagtgc caagctgagt gacaagtccc 601 gctatgacta ctttgcccgc acagtgcctc ctgacttctt ccaagccaag gccatggctg 661 agattctccg cttcttcaac tggacctatg tgtccactgt ggcgtctgag ggcgactatg 721 gcgagacagg cattgaggcc tttgagctag aggctcgtgc ccgcaacatc tgtgtggcca 781 cctcggagaa agtgggccgt gccatgagcc gcgcggcctt tgagggtgtg gtgcgagccc 841 tgctgcagaa gcccagtgcc cgcgtggctg tcctgttcac ccgttctgag gatgcccggg 901 agctgcttgc tgccagccag cgcctcaatg ccagcttcac ctgggtggcc agtgatggtt 961 ggggggccct ggagagtgtg gtggcaggca gtgagggggc tgctgagggt gctatcacca 1021 tcgagctggc ctcctacccc atcagtgact ttgcctccta cttccagagc ctggaccctt 1081 ggaacaacag ccggaacccc tggttccgtg aattctggga gcagaggttc cgctgcagct 1141 tccggcagcg agactgcgca gcccactctc tccgggctgt gccctttgag caggagtcca 1201 agatcatgtt tgtggtcaat gcagtgtacg ccatggccca tgcgctccac aacatgcacc 1261 gtgccctctg ccccaacacc acccggctct gtgacgcgat gcggccagtt aacgggcgcc 1321 gcctctacaa ggactttgtg ctcaacgtca agtttgatgc cccctttcgc ccagctgaca 1381 cccacaatga ggtccgcttt gaccgctttg gtgatggtat tggccgctac aacatcttca 1441 cctatctgcg tgcaggcagt gggcgctatc gctaccagaa ggtgggctac tgggcagaag 1501 gcttgactct ggacaccagc ctcatcccat gggcctcacc ctcagccggc cccctgcccg 1561 cctctcgctg cagtgagccc tgcctccaga atgaggtgaa gagtgtgcag ccgggcgaag 1621 tctgctgctg gctctgcatt ccgtgccagc cctatgagta ccgattggac gaattcactt 1681 gcgctgattg tggcctgggc tactggccca atgccagcct gactggctgc ttcgaactgc 1741 cccaggagta catccgctgg ggcgatgcct gggctgtggg acctgtcacc atcgcctgcc 1801 tcggtgccct ggccaccctc tttgtgctgg gtgtctttgt gcggcacaat gccacaccag 1861 tggtcaaggc ctcaggtcgg gagctctgct acatcctgct gggtggtgtc ttcctctgct 1921 actgcatgac cttcatcttc attgccaagc catccacggc agtgtgtacc ttacggcgtc 1981 ttggtttggg cactgccttc tctgtctgct actcagccct gctcaccaag accaaccgca 2041 ttgcacgcat cttcggtggg gcccgggagg gtgcccagcg gccacgcttc atcagtcctg 2101 cctcacaggt ggccatctgc ctggcactta tctcgggcca gctgctcatc gtggtcgcct 2161 ggctggtggt ggaggcaccg ggcacaggca aggagacagc ccccgaacgg cgggaggtgg 2221 tgacactgcg ctgcaaccac cgcgatgcaa gtatgttggg ctcgctggcc tacaatgtgc 2281 tcctcatcgc gctctgcacg ctttatgccT TCAAGACTCG CAAGTGCCCC GAAAACTTCA 2341 ACGAGGCCAA GTTCATTGGC TTCACCATGT ACACCACCTG CATCATCTGG CTGGCATTCC 2401 TGCCCATCTT CTATGTCACC TCCAGTGACT ACCGGGTACA GACCACCACC ATGTGCGTGT 2461 CAGTCAGCCT CAGCGGCTCC GTGGTGCTTG GCTGCCTCTT TGCGCCCAAG CTGCACATCA 2521 TCCTCTTCCA GCCGCAGAAG AACGTGGTTA GCCACCGGGC ACCCACCAGC CGCTTTGGCA 2581 GTGCTGCTGC CAGGGCCAGC TCCAGCCTTG GCCAAGGGTC TGGCTCCCAG TTTGTCCCCA 2641 CTGTTTGCAA TGGCCGTGAG GTGGTGGACT CGACAACGTC ATCGCTTTGA GACCCAGCTT 2701 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2761 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2821 CTTGTGGAAA GGACGAAAAC GATTACCACG TACTCTGTGC ACGCGTTAAG TCgacaatca 2881 acctctggat tacaaaattt gtgaaagatt