Construct: ORF TRCN0000489041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020629.1_s317c1
- DNA Barcode:
- CACATTGGCTCCGGCCTGGTTTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- WEE1 (7465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489041
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7465 | WEE1 | WEE1 G2 checkpoint kinase | NM_003390.4 | 99.9% | 100% | 252C>G |
| 2 | human | 7465 | WEE1 | WEE1 G2 checkpoint kinase | NM_001143976.1 | 66.8% | 66.8% | 0_1ins642 |
| 3 | mouse | 22390 | Wee1 | WEE 1 homolog 1 (S. pombe) | NM_009516.3 | 86.8% | 90.2% | (many diffs) |
| 4 | mouse | 22390 | Wee1 | WEE 1 homolog 1 (S. pombe) | XM_006507585.3 | 79.9% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2010
- ORF length:
- 1938
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcttc ctgagccgac agcagccgcc gccaccccgc cgcgccgggg 121 cggcctgcac cttgcggcag aagctgatct tctcgccctg cagcgactgt gaggaggagg 181 aagaagagga ggaggaggag ggcagcggcc acagcaccgg ggaggactcg gcctttcaag 241 agcccgactc gccgctgccg cccgcgcgga gccccacgga gcccgggccc gagcgccgcc 301 gctcgcccgg gccggccccc gggagccccg gcgagctgga ggaggacctg ttgctgcccg 361 gcgcctgccc gggcgcggac gaggcgggcg gtggggcgga gggcgactcg tgggaggagg 421 agggcttcgg ctcctcgtcg ccggtcaagt cgccggcggc cccctacttc ctgggtagct 481 ctttctcgcc ggtgcgctgc ggcggcccag gagatgcgtc gccgcggggt tgcggggcgc 541 gccgggcggg cgaaggccgc cgctcgccgc ggccggacca cccgggcacc ccgccacaca 601 agaccttccg caagctgcga ctcttcgaca ccccgcacac gcccaagagt ttgctctcca 661 aagctcgggg aattgattcc agctctgtta aactccgggg tagttctctc ttcatggata 721 cagaaaaatc aggaaaaagg gaatttgatg tgcgacagac tcctcaagtg aatattaatc 781 cttttactcc ggattctttg ttgcttcatt cctcaggaca gtgtcgtcgt agaaagagaa 841 cgtattggaa tgattcctgt ggtgaagaca tggaagccag tgattatgag cttgaagatg 901 aaacaagacc tgctaagaga attacaatta ctgaaagcaa tatgaagtcc cggtatacaa 961 cagaatttca tgagctagag aaaatcggct ctggagaatt tggttctgta tttaagtgtg 1021 tgaagaggct ggatggatgc atttatgcca ttaagcgatc aaaaaagcca ttggcgggct 1081 ctgttgatga gcagaacgct ttgagagaag tatatgctca tgcagtgctt ggacagcatt 1141 ctcatgtagt tcgatatttc tctgcgtggg cagaagatga tcatatgctt atacagaatg 1201 aatattgtaa tggtggaagt ttagctgatg ctataagtga aaactacaga atcatgagtt 1261 actttaaaga agcagagttg aaggatctcc ttttgcaagt tggccgaggc ttgaggtata 1321 ttcattcaat gtctttggtt cacatggata taaaacctag taatattttc atatctcgaa 1381 cctcaatccc aaatgctgcc tctgaagaag gagacgaaga tgattgggca tccaacaaag 1441 ttatgtttaa aataggtgat cttgggcatg taacaaggat ctccagtcca caagttgaag 1501 agggcgatag tcgttttctt gcaaatgaag ttttacagga gaattatacc catctaccaa 1561 aagcagatat ttttgcgctt gccctcacag tggtatgtgc tgctggtgct gaaccTCTTC 1621 CGAGAAATGG AGATCAATGG CATGAAATCA GACAGGGTAG ATTACCTCGG ATACCACAAG 1681 TGCTTTCCCA AGAATTTACA GAGTTGCTAA AAGTTATGAT TCATCCAGAT CCAGAGAGAA 1741 GACCTTCAGC AATGGCACTG GTAAAGCATT CAGTATTGCT GTCCGCTTCT AGAAAGAGTG 1801 CAGAACAATT ACGAATAGAA TTGAATGCCG AAAAGTTCAA AAATTCACTT TTACAAAAAG 1861 AACTCAAGAA AGCACAGATG GCAAAAGCTG CAGCTGAGGA AAGAGCACTC TTCACTGACC 1921 GGATGGCCAC TAGGTCCACC ACCCAGAGTA ATAGAACATC TCGACTTATT GGAAAGAAAA 1981 TGAACCGCTC TGTCAGCCTT ACTATATACT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 2041 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2101 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 2161 CATTGGCTCC GGCCTGGTTT ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 2221 ttgtgaaaga tt