Construct: ORF TRCN0000489092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019690.1_s317c1
- DNA Barcode:
- CTTCAAACAGAGGGAACCCCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSNK1A1 (1452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001892.6 | 99.8% | 99.4% | 850C>T;1011_1012insG |
| 2 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001271741.2 | 95.6% | 95.5% | 850C>T;972_974delTTT;975_976ins40 |
| 3 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001025105.3 | 92.1% | 91.8% | 455_538del;934C>T;1095_1096insG |
| 4 | human | 122011 | CSNK1A1L | casein kinase 1 alpha 1 like | NM_145203.6 | 91.3% | 89.9% | (many diffs) |
| 5 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001271742.2 | 67.7% | 67.4% | (many diffs) |
| 6 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526388.2 | 94.7% | 99.4% | (many diffs) |
| 7 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | NM_146087.2 | 90.6% | 95.5% | (many diffs) |
| 8 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526384.2 | 87.5% | 91.8% | (many diffs) |
| 9 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526385.2 | 87.2% | 91.5% | (many diffs) |
| 10 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526383.3 | 85.4% | 89.5% | (many diffs) |
| 11 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_011247048.2 | 85.3% | 89.5% | (many diffs) |
| 12 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526386.3 | 84.8% | 88% | (many diffs) |
| 13 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526387.2 | 83.7% | 88.2% | (many diffs) |
| 14 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XR_386023.3 | 13.8% | (many diffs) | |
| 15 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XR_386022.3 | 13.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1089
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcg agtagcagcg gctccaaggc tgaattcatt gtcggaggga 121 aatataaact ggtacggaag atcgggtctg gctccttcgg ggacatctat ttggcgatca 181 acatcaccaa cggcgaggaa gtggcagtga agctagaatc tcagaaggcc aggcatcccc 241 agttgctgta cgagagcaag ctctataaga ttcttcaagg tggggttggc atcccccaca 301 tacggtggta tggtcaggaa aaagactaca atgtactagt catggatctt ctgggaccta 361 gcctcgaaga cctcttcaat ttctgttcaa gaaggttcac aatgaaaact gtacttatgt 421 tagctgacca gatgatcagt agaattgaat atgtgcatac aaagaatttt atacacagag 481 acattaaacc agataacttc ctaatgggta ttgggcgtca ctgtaataag ttattcctta 541 ttgattttgg tttggccaaa aagtacagag acaacaggac aaggcaacac ataccataca 601 gagaagataa aaacctcact ggcactgccc gatatgctag catcaatgca catcttggta 661 ttgagcagag tcgccgagat gacatggaat cattaggata tgttttgatg tattttaata 721 gaaccagccT GCCATGGCAA GGGCTAAAGG CTGCAACAAA GAAACAAAAA TATGAAAAGA 781 TTAGTGAAAA GAAGATGTCC ACGCCTGTTG AAGTTTTATG TAAGGGGTTT CCTGCAGAAT 841 TTGCGATGTA CTTAAACTAT TGTCGTGGGC TACGCTTTGA GGAAGCCCCA GATTACATGT 901 ATCTGAGGCA GCTATTCCGC ATTTTTTTCA GGACCCTGAA CCATCAATAT GACTACACAT 961 TTGATTGGAC AATGTTAAAG CAGAAAGCAG CACAGCAGGC AGCCTCTTCC AGTGGGCAGG 1021 GTCAGCAGGC CCAAACCCCC ACAGGCAAGC AAACTGACAA AACCAAGAGT AACATGAAAG 1081 GTTTCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTTCAAACA GAGGGAACCC CTATCACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt