Transcript: Human NM_145203.6

Homo sapiens casein kinase 1 alpha 1 like (CSNK1A1L), mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
CSNK1A1L (122011)
Length:
2406
CDS:
409..1422

Additional Resources:

NCBI RefSeq record:
NM_145203.6
NBCI Gene record:
CSNK1A1L (122011)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147607 ATACATGAAAACGTAGCCTA pXPR_003 AGG 622 61% 1 0.7494 CSNK1A1L CSNK1A1L 77383
2 BRDN0001146686 CTGACCATACCAGTGCATGT pXPR_003 GGG 226 22% 1 0.5745 CSNK1A1L CSNK1A1L 77382
3 BRDN0001145992 GAGGGAAATACAAACTGGTG pXPR_003 CGG 57 6% 1 -0.0952 CSNK1A1L CSNK1A1L 77381
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_145203.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195665 CACGAATTGGTGGAGACATTG pLKO.1 1711 3UTR 100% 10.800 15.120 N CSNK1A1L n/a
2 TRCN0000007024 CCCAAAGTGTAGCCTCTGTAA pLKO.1 1601 3UTR 100% 4.950 6.930 N CSNK1A1L n/a
3 TRCN0000007027 TGGAATCCTTAGGCTACGTTT pLKO.1 1019 CDS 100% 4.950 6.930 N CSNK1A1L n/a
4 TRCN0000007026 GCCATGTACTTGAACTACTGT pLKO.1 1177 CDS 100% 3.000 4.200 N CSNK1A1L n/a
5 TRCN0000195180 CTATATCATGTTCTCGGTATT pLKO.1 2057 3UTR 100% 10.800 7.560 N CSNK1A1L n/a
6 TRCN0000196806 GCAAAGGTTGTAAATGCTTAT pLKO.1 1821 3UTR 100% 10.800 7.560 N CSNK1A1L n/a
7 TRCN0000197181 GCTTACCTGTGAAGACATAAG pLKO.1 1992 3UTR 100% 10.800 7.560 N CSNK1A1L n/a
8 TRCN0000195022 CAGAATTGAATACGTGCATAC pLKO.1 774 CDS 100% 6.000 4.200 N CSNK1A1L n/a
9 TRCN0000007025 GAGAGCAAACTCTACACGATT pLKO.1 586 CDS 100% 4.950 3.465 N CSNK1A1L n/a
10 TRCN0000007028 GCTGGAATCTCAGAAGGTCAA pLKO.1 546 CDS 100% 4.050 2.835 N CSNK1A1L n/a
11 TRCN0000195725 CCGAGACATTAAACCAGATAA pLKO.1 810 CDS 100% 13.200 7.920 N CSNK1A1L n/a
12 TRCN0000195315 CCAGATGATCAGCAGAATTGA pLKO.1 762 CDS 100% 5.625 3.375 N CSNK1A1L n/a
13 TRCN0000023795 CCACCAATATGACTACACATT pLKO.1 1275 CDS 100% 4.950 2.970 N Csnk1a1 n/a
14 TRCN0000276636 CCACCAATATGACTACACATT pLKO_005 1275 CDS 100% 4.950 2.970 N Csnk1a1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145203.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09471 pDONR223 100% 99.8% 99.4% None 126C>A;674G>A n/a
2 ccsbBroad304_09471 pLX_304 0% 99.8% 99.4% V5 126C>A;674G>A n/a
3 TRCN0000479467 AACCGTGTACGGGCCCCCAACCGA pLX_317 39.4% 99.8% 99.4% V5 126C>A;674G>A n/a
4 ccsbBroadEn_15232 pDONR223 0% 99.8% 99.4% None 126C>A;674G>A n/a
5 ccsbBroad304_15232 pLX_304 0% 99.8% 99.4% V5 126C>A;674G>A n/a
6 TRCN0000479515 GTCTGAATTAGTTATATTGCCGTG pLX_317 36.9% 99.8% 99.4% V5 126C>A;674G>A n/a
7 TRCN0000488459 AGGGTACAACTCATGCCAATTGTA pLX_317 28.3% 99.8% 99.4% V5 (not translated due to prior stop codon) 126C>A;674G>A n/a
8 TRCN0000489911 TACCACATCGATAAACGCTCGCAG pLX_317 34.5% 99.7% 99.1% V5 126C>A;674G>A;1011_1012insG n/a
9 TRCN0000489949 GCATCGCACCCAACTGGGATACTC pLX_317 39.7% 91.4% 90.2% V5 (many diffs) n/a
10 ccsbBroadEn_06055 pDONR223 100% 91.3% 90.2% None (many diffs) n/a
11 ccsbBroad304_06055 pLX_304 66.4% 91.3% 90.2% V5 (many diffs) n/a
12 TRCN0000477459 AATTTCCATTAGTCTTCCTTATAA pLX_317 42.5% 91.3% 90.2% V5 (many diffs) n/a
13 TRCN0000488414 CCCTCATTAATCGTGTGGTTAAAT pLX_317 34.4% 91.3% 90.5% V5 (not translated due to prior stop codon) (many diffs) n/a
14 TRCN0000492345 TCCACGATCACCACTAATTCCGCC pLX_317 46% 91.3% 90.2% V5 (not translated due to prior stop codon) (many diffs) n/a
15 TRCN0000489092 CTTCAAACAGAGGGAACCCCTATC pLX_317 35.2% 91.3% 89.9% V5 (many diffs) n/a
16 ccsbBroad304_14599 pLX_304 66.1% 90.9% 18.6% V5 (not translated due to prior stop codon) (many diffs) n/a
17 TRCN0000473547 CTCATCCGGTGCGATGATGCCAAA pLX_317 42.4% 90.8% 18.6% V5 (not translated due to prior stop codon) (many diffs) n/a
18 ccsbBroadEn_14599 pDONR223 100% 90.4% 18.6% None (many diffs) n/a
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