Construct: ORF TRCN0000489098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021671.1_s317c1
- DNA Barcode:
- TCCCTCCACCTGTGGTATTTTTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ESR1 (2099)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2099 | ESR1 | estrogen receptor 1 | NM_000125.3 | 99.6% | 100% | 1782G>A;1785_1786insTGATC |
| 2 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001122740.1 | 99.6% | 100% | 1782G>A;1785_1786insTGATC |
| 3 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001122741.1 | 99.6% | 100% | 1782G>A;1785_1786insTGATC |
| 4 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001122742.1 | 99.6% | 100% | 1782G>A;1785_1786insTGATC |
| 5 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535543.2 | 99.5% | 100% | (many diffs) |
| 6 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535544.2 | 99.5% | 100% | (many diffs) |
| 7 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535545.2 | 99.5% | 100% | (many diffs) |
| 8 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010376.1 | 99.5% | 100% | (many diffs) |
| 9 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010377.1 | 99.5% | 100% | (many diffs) |
| 10 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010378.1 | 99.5% | 100% | (many diffs) |
| 11 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010379.1 | 99.5% | 100% | (many diffs) |
| 12 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010380.1 | 99.5% | 100% | (many diffs) |
| 13 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010381.1 | 99.5% | 100% | (many diffs) |
| 14 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001291241.1 | 99.4% | 99.8% | 756_757insGGT;1779G>A;1782_1783insTGATC |
| 15 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001291230.1 | 99.3% | 99.6% | 644_649delGTAATA;1788G>A;1791_1792insTGATC |
| 16 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535547.2 | 79.3% | 76.4% | (many diffs) |
| 17 | human | 2099 | ESR1 | estrogen receptor 1 | XM_006715374.3 | 77.9% | 76.8% | (many diffs) |
| 18 | human | 2099 | ESR1 | estrogen receptor 1 | XM_006715375.3 | 70.5% | 70.9% | (many diffs) |
| 19 | human | 2099 | ESR1 | estrogen receptor 1 | XR_002956266.1 | 65.3% | (many diffs) | |
| 20 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010382.2 | 62.8% | 63.1% | (many diffs) |
| 21 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535549.2 | 57.8% | 57.1% | (many diffs) |
| 22 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010383.1 | 55.5% | 55.7% | (many diffs) |
| 23 | human | 2099 | ESR1 | estrogen receptor 1 | XR_001743223.2 | 53.1% | (many diffs) | |
| 24 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001328100.2 | 50.5% | 47.9% | (many diffs) |
| 25 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | NM_001302531.1 | 85.4% | 88.9% | (many diffs) |
| 26 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | NM_001302532.1 | 85.4% | 88.9% | (many diffs) |
| 27 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | NM_007956.5 | 85.4% | 88.9% | (many diffs) |
| 28 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_006512433.3 | 85.4% | 88.9% | (many diffs) |
| 29 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_006512434.3 | 85.4% | 88.9% | (many diffs) |
| 30 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_006512435.2 | 85.4% | 88.9% | (many diffs) |
| 31 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_011243066.2 | 85.4% | 88.9% | (many diffs) |
| 32 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_011243067.2 | 85.4% | 88.9% | (many diffs) |
| 33 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_011243068.2 | 85.4% | 88.9% | (many diffs) |
| 34 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_017313797.1 | 54% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 1887
- ORF length:
- 1785
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccggat ccccacggac catgaccatg accctccaca 121 ccaaagcatc tgggatggcc ctactgcatc agatccaagg gaacgagctg gagcccctga 181 accgtccgca gctcaagatc cccctggagc ggcccctggg cgaggtgtac ctggacagca 241 gcaagcccgc cgtgtacaac taccccgagg gcgccgccta cgagttcaac gccgcggccg 301 ccgccaacgc gcaggtctac ggtcagaccg gcctccccta cggccccggg tctgaggctg 361 cggcgttcgg ctccaacggc ctggggggtt tccccccact caacagcgtg tctccgagcc 421 cgctgatgct actgcacccg ccgccgcagc tgtcgccttt cctgcagccc cacggccagc 481 aggtgcccta ctacctggag aacgagccca gcggctacac ggtgcgcgag gccggcccgc 541 cggcattcta caggccaaat tcagataatc gacgccaggg tggcagagaa agattggcca 601 gtaccaatga caagggaagt atggctatgg aatctgccaa ggagactcgc tactgtgcag 661 tgtgcaatga ctatgcttca ggctaccatt atggagtctg gtcctgtgag ggctgcaagg 721 ccttcttcaa gagaagtatt caaggacata acgactatat gtgtccagcc accaaccagt 781 gcaccattga taaaaacagg aggaagagct gccaggcctg ccggctccgc aaatgctacg 841 aagtgggaat gatgaaaggt gggatacgaa aagaccgaag aggagggaga atgttgaaac 901 acaagcgcca gagagatgat ggggagggca ggggtgaagt ggggtctgct ggagacatga 961 gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg 1021 ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac 1081 tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga 1141 ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct 1201 ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc 1261 tgatgattgg tctcgtctgg cgctccatgg agcacccagg gaagctactg tttgctccta 1321 acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca 1381 tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt 1441 gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga 1501 agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca gacactttga 1561 tccacctgat ggccaaggca ggccTGACCC TGCAGCAGCA GCACCAGCGG CTGGCCCAGC 1621 TCCTCCTCAT CCTCTCCCAC ATCAGGCACA TGAGTAACAA AGGCATGGAG CATCTGTACA 1681 GCATGAAGTG CAAGAACGTG GTGCCCCTCT ATGACCTGCT GCTGGAGATG CTGGACGCCC 1741 ACCGCCTACA TGCGCCCACT AGCCGTGGAG GGGCATCCGT GGAGGAGACG GACCAAAGCC 1801 ACTTGGCCAC TGCGGGCTCT ACTTCATCGC ATTCCTTGCA AAAGTATTAC ATCACGGGGG 1861 AGGCAGAGGG TTTCCCTGCC ACAGTCTGAT CTAGAAAGGG TGGGCGCGCC GACCCAGCTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGATCCC TCCACCTGTG GTATTTTTAG ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt