Construct: ORF TRCN0000489110
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020904.1_s317c1
- DNA Barcode:
- ATCCGTGTTTTGCTAGACCATCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CRHR1 (1394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489110
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_004382.5 | 100% | 100% | |
| 2 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001145148.2 | 96.6% | 96.6% | 1063_1064ins42 |
| 3 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001145146.2 | 93.4% | 93.4% | 433_519del |
| 4 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001145147.2 | 90.3% | 90.1% | 121_122ins120 |
| 5 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001303020.1 | 75.6% | 73.7% | 0_1ins217;24_25ins86 |
| 6 | human | 104909134 | LINC02210-CRHR1 | LINC02210-CRHR1 readthrough | NM_001303016.1 | 62.2% | 50.3% | (many diffs) |
| 7 | human | 104909134 | LINC02210-CRHR1 | LINC02210-CRHR1 readthrough | NM_001256299.3 | 57.8% | 57.8% | 0_1ins525 |
| 8 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001303018.2 | 57.8% | 57.8% | 0_1ins525 |
| 9 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | NM_007762.5 | 91% | 97.5% | (many diffs) |
| 10 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | NM_001313928.1 | 82.2% | 87.7% | (many diffs) |
| 11 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XM_017314246.1 | 75.8% | 72% | (many diffs) |
| 12 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XM_017314247.1 | 75.8% | 72% | (many diffs) |
| 13 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | NM_001313929.1 | 45.9% | 50.1% | (many diffs) |
| 14 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XR_001779871.1 | 40.7% | (many diffs) | |
| 15 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XR_001779869.1 | 34% | (many diffs) | |
| 16 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XR_001779870.1 | 32.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1317
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaggg cacccgcagc tccgtctcgt caaggccctt ctccttctgg 121 ggctgaaccc cgtctctgcc tccctccagg accagcactg cgagagcctg tccctggcca 181 gcaacatctc aggactgcag tgcaacgcat ccgtggacct cattggcacc tgctggcccc 241 gcagccctgc ggggcagcta gtggttcggc cctgccctgc ctttttctat ggtgtccgct 301 acaataccac aaacaatggc taccgggagt gcctggccaa tggcagctgg gccgcccgcg 361 tgaattactc cgagtgccag gagatcctca atgaggagaa aaaaagcaag gtgcactacc 421 atgtcgcagt catcatcaac tacctgggcc actgtatctc cctggtggcc ctcctggtgg 481 cctttgtcct ctttctgcgg ctcaggagca tccggtgcct gcgaaacatc atccactgga 541 acctcatctc cgccttcatc ctgcgcaacg ccacctggtt cgtggtccag ctaaccatga 601 gccccgaggt ccaccagagc aacgtgggct ggtgcaggtt ggtgacagcc gcctacaact 661 acttccatgt gaccaacttc ttctggatgt tcggcgaggg ctgctacctg cacacagcca 721 tcgtgctcac ctactccact gaccggctgc gcaaatggat gttcatctgc attggctggg 781 gtgtgccctt ccccatcatt gtggcctggg ccattgggaa gctgtactac gacaatgaga 841 agtgctggtt tggcaaaagg cctggggtgt acaccgacta catctaccag ggccccatga 901 tcctggtcct gctgatcaat ttcatcttcc ttttcaacat cgtccgcatc cTCATGACCA 961 AGCTCCGGGC ATCCACCACG TCTGAGACCA TTCAGTACAG GAAGGCTGTG AAAGCCACTC 1021 TGGTGCTGCT GCCCCTCCTG GGCATCACCT ACATGCTGTT CTTCGTCAAT CCCGGGGAGG 1081 ATGAGGTCTC CCGGGTCGTC TTCATCTACT TCAACTCCTT CCTGGAATCC TTCCAGGGCT 1141 TCTTTGTGTC TGTGTTCTAC TGTTTCCTCA ATAGTGAGGT CCGTTCTGCC ATCCGGAAGA 1201 GGTGGCACCG GTGGCAGGAC AAGCACTCGA TCCGTGCCCG AGTGGCCCGT GCCATGTCCA 1261 TCCCCACCTC CCCAACCCGT GTCAGCTTTC ACAGCATCAA GCAGTCCACA GCAGTCTAGG 1321 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAATCCG TGTTTTGCTA GACCATCTCA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt