Construct: ORF TRCN0000489125
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020213.1_s317c1
- DNA Barcode:
- GTATTTGGCCCGATCACCTCAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NAGK (55577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489125
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_017567.6 | 99.9% | 100% | 273G>C |
| 2 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_001330425.3 | 85% | 85.1% | 0_1ins153;120G>C |
| 3 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_001330426.2 | 85% | 85.1% | 0_1ins153;120G>C |
| 4 | human | 55577 | NAGK | N-acetylglucosamine kinase | NM_001365466.2 | 85% | 85.1% | 0_1ins153;120G>C |
| 5 | human | 55577 | NAGK | N-acetylglucosamine kinase | XM_011532957.1 | 85% | 85.1% | 0_1ins153;120G>C |
| 6 | human | 55577 | NAGK | N-acetylglucosamine kinase | XR_939694.1 | 59.9% | (many diffs) | |
| 7 | human | 55577 | NAGK | N-acetylglucosamine kinase | XM_017004440.1 | 55.5% | 49.7% | (many diffs) |
| 8 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | NM_019542.2 | 86.9% | 91.5% | (many diffs) |
| 9 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | NM_001347315.1 | 82.2% | 84.8% | (many diffs) |
| 10 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_006506408.2 | 77.8% | 82.2% | (many diffs) |
| 11 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | NM_001164187.1 | 73.6% | 78.1% | (many diffs) |
| 12 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_006506409.2 | 63.5% | 62.1% | (many diffs) |
| 13 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_006506410.1 | 63.5% | 62.1% | (many diffs) |
| 14 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_017321685.1 | 63.5% | 62.1% | (many diffs) |
| 15 | mouse | 56174 | Nagk | N-acetylglucosamine kinase | XM_017321686.1 | 38.6% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1104
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgcg atctatgggg gtgtagaggg gggaggcaca cgatccgagg 121 tccttttagt ctcagaggat gggaagatcc tggcagaagc agatggactg agcacaaacc 181 actggctgat cgggacagac aagtgtgtgg agaggatcaa tgagatggtg aacagggcca 241 aacggaaagc aggggtggat cctctggtac cgctgcgaag cttgggccta tctctgagcg 301 gtggggacca ggaggacgcg gggaggatcc tgatcgagga gctcagggac cgatttccct 361 acctgagtga aagctactta atcaccaccg atgccgccgg ctccatcgcc acagctacac 421 cggatggtgg agttgtgctc atatctggaa caggctccaa ctgcaggctc atcaaccctg 481 atggctccga gagtggctgc ggcggctggg gccatatgat gggtgatgag ggttcagcct 541 actggatcgc acaccaagca gtgaaaatag tgtttgactc cattgacaac ctagaggcgg 601 ctcctcatga tatcggctac gtcaaacagg ccatgttcca ctatttccag gtgccagatc 661 ggctagggat actcactcac ctgtataggg actttgataa atgcaggttt gctgggtttt 721 gccggaaaat tgcagaaggt gctcagcagg gagaccccct ttcccgctat atcTTCAGGA 781 AGGCTGGGGA GATGCTGGGC AGACACATCG TAGCAGTGTT GCCCGAGATT GACCCGGTCT 841 TGTTCCAGGG CAAGATTGGA CTCCCCATCC TGTGCGTGGG CTCTGTGTGG AAGAGCTGGG 901 AGCTGCTGAA GGAAGGTTTT CTTCTGGCGC TGACCCAGGG CAGAGAGATC CAGGCTCAGA 961 ACTTCTTCTC CAGCTTCACC CTGATGAAGC TGAGGCACTC CTCCGCTCTG GGTGGGGCCA 1021 GCCTAGGGGC CAGGCACATC GGGCACCTCC TCCCCATGGA CTATAGCGCC AATGCCATTG 1081 CCTTCTATTC CTACACCTTT TCCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTATTTGG 1261 CCCGATCACC TCAACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt