Construct: ORF TRCN0000489127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019227.1_s317c1
- DNA Barcode:
- TACAGCGATCATGGTAACCACTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR155 (151556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151556 | GPR155 | G protein-coupled receptor 155 | NM_001033045.4 | 99.9% | 100% | 2460C>T |
| 2 | human | 151556 | GPR155 | G protein-coupled receptor 155 | NM_001267050.2 | 99.9% | 100% | 2460C>T |
| 3 | human | 151556 | GPR155 | G protein-coupled receptor 155 | NM_152529.7 | 99.9% | 100% | 2460C>T |
| 4 | human | 151556 | GPR155 | G protein-coupled receptor 155 | XM_017003485.2 | 99.9% | 100% | 2460C>T |
| 5 | human | 151556 | GPR155 | G protein-coupled receptor 155 | XM_017003486.2 | 99.9% | 100% | 2460C>T |
| 6 | human | 151556 | GPR155 | G protein-coupled receptor 155 | XM_017003487.1 | 99.9% | 100% | 2460C>T |
| 7 | human | 151556 | GPR155 | G protein-coupled receptor 155 | NM_001267051.1 | 96.7% | 96.7% | 1181_1182ins84;2376C>T |
| 8 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | NM_001190297.2 | 85.4% | 89.8% | (many diffs) |
| 9 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | XM_006500106.1 | 85.4% | 89.8% | (many diffs) |
| 10 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | XM_006500104.1 | 84.2% | 88.5% | (many diffs) |
| 11 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | XM_006500105.1 | 84.2% | 88.5% | (many diffs) |
| 12 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | NM_001276444.1 | 82.7% | 86.8% | (many diffs) |
| 13 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | NM_001276443.1 | 77.1% | 79.7% | (many diffs) |
| 14 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | XM_006500107.2 | 75.9% | 78.5% | (many diffs) |
| 15 | mouse | 68526 | Gpr155 | G protein-coupled receptor 155 | XM_006500108.3 | 75.1% | 78.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2685
- ORF length:
- 2610
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaat tctaatttac ctgcagagaa cttaaccatt gcagtcaata 121 tgaccaagac tttgcctaca gcagtaacgc atggatttaa ttccactaat gacccacctt 181 caatgtcaat tacaaggctt tttccagcct tactggaatg ctttggcatt gtcctttgtg 241 gctacatagc aggaagggcc aatgtcataa catcaaccca ggccaaagga ctaggaaatt 301 ttgtctccag atttgcactt ccagctttat tattcaaaaa catggttgta cttaattttt 361 ccaatgtgga ctggtccttc ctatatagta tcttaattgc caaagcttct gtatttttca 421 ttgtatgtgt attaacctta ttggttgcca gtcctgatag tcgatttagc aaagctggac 481 tattccctat ttttgctaca caaagtaatg actttgcatt gggataccct atagttgaag 541 ctttatatca aactacatac ccagaatatc tccagtacat ttatttggtg gcaccaatat 601 ctcttatgat gttaaaccct atagggttta ttttctgtga aatccaaaag tggaaagaca 661 ctcaaaatgc ttctcaaaat aaaataaaaa ttgtgggact cggactcctg cgtgtattac 721 agaacccaat agtatttatg gtcttcattg gcatcgcctt caattttatt cttgatcgaa 781 aggtacctgt atatgtcgaa aattttcttg atggacttgg aaattctttt tctggatcag 841 ccctatttta tcttggtctc acgatggtgg gaaaaataaa gagactgaag aagtcggcat 901 ttgtagtact aattcttctc atcacagcta aacttctggt gctgccactt ctgtgcagag 961 aaatggtgga actcttggac aagggcgaca gtgtggtgaa ccatacaagt ttatcaaatt 1021 atgcatttct gtatggtgta tttcctgtag caccaggagt ggctatcttt gcaacacaat 1081 tcaacatgga agtagaaatt ataacctcag ggatggtgat aagcacattt gtgtctgctc 1141 ccatcatgta cgtttctgcc tggttactga cctttcccac tatggaccct aagccattgg 1201 catatgccat ccagaatgtt agttttgata taagtattgt cagcctgatc tccttgatct 1261 ggtctctggc tattcttctt ttgagtaaga aatataaaca gcttcctcat atgcttacaa 1321 ctaatttact cattgctcag tctattgtct gtgctggaat gatgatatgg aattttgtta 1381 aagaaaaaaa ttttgttgga caaattttgg tgtttgttct attgtacagc tccctctata 1441 gcacctacct gtggacaggc cttctagcaa tttctttgtt tcttttgaaa aagcgagaga 1501 gggtacaaat tcctgttgga ataatcataa tatctggctg gggaattcct gctctccttg 1561 ttggtgttct tttgataact ggaaaacaca atggagatag cattgactca gccttctttt 1621 atggaaaaga acagatgatc accacagcag tcaccctgtt ctgcagcatc ctgatagctg 1681 gcatatccct catgtgcatg aaccagactg cccaagcagg aagctatgaa ggtttcgatc 1741 agtctcagag ccacaaagtg gtggagcctg gaaatactgc ttttgaggag agtccagcac 1801 cagtaaatga accagaactt tttacaagct ctattccaga aacaagttgc tgctcctgct 1861 ccatgggaaa tggtgaatta cactgcccat caatagagcc aatagcaaac acaagcacca 1921 gtgagcctgt gattccttcg tttgagaaaa acaatcattg tgtgagtcgc tgtaactccc 1981 agagctgcat attagcccag gaagaagaac agtatctaca gagtggagac cagcaactga 2041 cccgacatgt gttgctgtgt ttacttctca tcattggcct gttcgctaat ctttccagtt 2101 gtttatggtg gctattcaac caagagcctg gaagacttta tgttgagtta cagtttttct 2161 gtgccgtgtt taactttggt cagggattta tttcctttgg aatctttgga ttagataaac 2221 atttaatcat cctgcctttc aaaagaagac ttgaattcct atggaacaat aaagacacag 2281 cagaaaaCAG GGATTCTCCT GTTTCAGAGG AAATAAAAAT GACCTGTCAA CAATTTATCC 2341 ATTATCACCG TGACCTCTGT ATCCGAAACA TTGTCAAAGA AAGAAGGTGT GGTGCAAAGA 2401 CTTCTGCTGG AACTTTCTGT GGCTGTGACC TGGTGAGCTG GCTAATTGAA GTCGGCCTTG 2461 CCTCCGACCG TGGTGAAGCT GTGATATACG GAGACAGGCT GGTACAAGGG GGAGTCATCC 2521 AACATATTAC CAATGAGTAT GAATTCCGGG ATGAGTACTT GTTTTACAGA TTTCTTCAAA 2581 AGAGTCCTGA ACAGAGTCCT CCTGCTATTA ATGCAAACAC TCTCCAACAG GAAAGATATA 2641 AAGAAATTGA GCATTCATCC CCACCCTCAC ATTCCCCTAA GACCGACCCA GCTTTCTTGT 2701 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2761 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2821 GAAAGGACGA TACAGCGATC ATGGTAACCA CTCCACGCGT TAAGTCgaca atcaacctct 2881 ggattacaaa atttgtgaaa gatt