Construct: ORF TRCN0000489154
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020673.1_s317c1
- DNA Barcode:
- CGCAACGTCAAACTGACCTGCCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKCA (5578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489154
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5578 | PRKCA | protein kinase C alpha | NM_002737.3 | 99.9% | 99.8% | 1702G>A |
| 2 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024836.2 | 93.3% | 92.5% | (many diffs) |
| 3 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024837.1 | 91.3% | 89.5% | (many diffs) |
| 4 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450829.1 | 86.4% | 85.8% | (many diffs) |
| 5 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450830.1 | 86.4% | 85.8% | (many diffs) |
| 6 | human | 5578 | PRKCA | protein kinase C alpha | XR_001752558.1 | 55.7% | (many diffs) | |
| 7 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024841.1 | 47.1% | 45.6% | (many diffs) |
| 8 | mouse | 18750 | Prkca | protein kinase C, alpha | NM_011101.3 | 91% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2088
- ORF length:
- 2016
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgac gttttcccgg gcaacgactc cacggcgtct caggacgtgg 121 ccaaccgctt cgcccgcaaa ggggcgctga ggcagaagaa cgtgcacgag gtgaaggacc 181 acaaattcat cgcgcgcttc ttcaagcagc ccaccttctg cagccactgc accgacttca 241 tctgggggtt tgggaaacaa ggcttccagt gccaagtttg ctgttttgtg gtccacaaga 301 ggtgccatga atttgttact ttttcttgtc cgggtgcgga taagggaccc gacactgatg 361 accccaggag caagcacaag ttcaaaatcc acacttacgg aagccccacc ttctgcgatc 421 actgtgggtc actgctctat ggacttatcc atcaagggat gaaatgtgac acctgcgata 481 tgaacgttca caagcaatgc gtcatcaatg tccccagcct ctgcggaatg gatcacactg 541 agaagagggg gcggatttac ctaaaggctg aggttgctga tgaaaagctc catgtcacag 601 tacgagatgc aaaaaatcta atccctatgg atccaaacgg gctttcagat ccttatgtga 661 agctgaaact tattcctgat cccaagaatg aaagcaagca aaaaaccaaa accatccgct 721 ccacactaaa tccgcagtgg aatgagtcct ttacattcaa attgaaacct tcagacaaag 781 accgacgact gtctgtagaa atctgggact gggatcgaac aacaaggaat gacttcatgg 841 gatccctttc ctttggagtt tcggagctga tgaagatgcc ggccagtgga tggtacaagt 901 tgcttaacca agaagaaggt gagtactaca acgtacccat tccggaaggg gacgaggaag 961 gaaacatgga actcaggcag aaattcgaga aagccaaact tggccctgct ggcaacaaag 1021 tcatcagtcc ctctgaagac aggaaacaac cttccaacaa ccttgaccga gtgaaactca 1081 cggacttcaa tttcctcatg gtgttgggaa aggggagttt tggaaaggtg atgcttgccg 1141 acaggaaggg cacagaagaa ctgtatgcaa tcaaaatcct gaagaaggat gtggtgattc 1201 aggatgatga cgtggagtgc accatggtag aaaagcgagt cttggccctg cttgacaaac 1261 ccccgttctt gacgcagctg cactcctgct tccagacagt ggatcggctg tacttcgtca 1321 tggaatatgt caacggtggg gacctcatgt accacattca gcaagtagga aaatttaagg 1381 aaccacaagc agtattctat gcggcagaga tttccatcgg attgttcttt cttcataaaa 1441 gaggaatcat ttatagggat ctgaagttag ataacgtcat gttggattca gaaggacata 1501 tcaaaattgc tgactttggg atgtgcaagg aacacatgat ggatggagtc acgaccagga 1561 ccttctgtgg gactccagat tatatcgccc cagagataat cgcttatcag ccgtatggaa 1621 aatctgtgga ctggtgggcc tatggcgtcc tgttgtatga aatgcttgcc gggcagcctc 1681 catttGATGG TGAAGATGAA GACGAGCTAT TTCAGTCTAT CATGGAGCAC AACGTTTCCT 1741 ATCCAAAATC CTTGTCCAAG GAGGCTGTTT CTATCTGCAA AGGACTGATG ACCAAACACC 1801 CAGCCAAGCG GCTGGGCTGT GGGCCTGAGG GGGAGAGGGA CGTGAGAGAG CATGCCTTCT 1861 TCCGGAGGAT CGACTGGGAA AAACTGGAGA ACAGGGAGAT CCAGCCACCA TTCAAGCCCA 1921 AAGTGTGTGG CAAAGGAGCA GAGAACTTTG ACAAGTTCTT CACACGAGGA CAGCCCGTCT 1981 TAACACCACC TGATCAGCTG GTTATTGCTA ACATAGACCA GTCTGATTTT GAAGGGTTCT 2041 CGTATGTCAA CCCCCAGTTT GTGCACCCCA TCTTACAGAG TGCAGTATAG AACCCAGCTT 2101 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2161 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2221 CTTGTGGAAA GGACGACGCA ACGTCAAACT GACCTGCCAT ACGCGTTAAG TCgacaatca 2281 acctctggat tacaaaattt gtgaaagatt