Construct: ORF TRCN0000489157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020549.1_s317c1
- DNA Barcode:
- CTATACTAGACCGTTATTCTAATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKD2 (25865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489157
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079882.1 | 74.6% | 74.6% | 1_549del |
| 2 | human | 25865 | PRKD2 | protein kinase D2 | XM_005258716.2 | 74.6% | 74.6% | 1_549del |
| 3 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079880.1 | 61.2% | 61.2% | 1_1020del |
| 4 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079881.1 | 61.2% | 61.2% | 1_1020del |
| 5 | human | 25865 | PRKD2 | protein kinase D2 | NM_016457.4 | 61.2% | 61.2% | 1_1020del |
| 6 | mouse | 101540 | Prkd2 | protein kinase D2 | XM_006539444.1 | 85.8% | 92% | (many diffs) |
| 7 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_001252458.1 | 54% | 57.9% | (many diffs) |
| 8 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_178900.4 | 54% | 57.9% | (many diffs) |
| 9 | mouse | 101540 | Prkd2 | protein kinase D2 | XR_391036.1 | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1686
- ORF length:
- 1614
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgag tcagaggact ccggtgtcat ccctggctcc cactcagaga 121 atgcgctcca cgccagtgag gaggaggaag gcgagggagg caaggcccag agctccctgg 181 ggtacatccc cctaatgagg gtggtgcaat cggtgcgaca cacgacgcgg aaatccagca 241 ccacgctgcg ggagggttgg gtggttcatt acagcaacaa ggacacgctg agaaagcggc 301 actattggcg cctggactgc aagtgtatca cgctcttcca gaacaacacg accaacagat 361 actataagga aattccgctg tcagaaatcc tcacggtgga gtccgcccag aacttcagcc 421 ttgtgccgcc gggcaccaac ccacactgct ttgagatcgt cactgccaat gccacctact 481 tcgtgggcga gatgcctggc gggactccgg gtgggccaag tgggcagggg gctgaggccg 541 cccggggctg ggagacagcc atccgccagg ccctgatgcc cgtcatcctt caggacgcac 601 ccagcgcccc aggccacgcg ccccacagac aagcttctct gagcatctct gtgtccaaca 661 gtcagatcca agagaatgtg gacattgcca ctgtctacca gatcttccct gacgaagtgc 721 tgggctcagg gcagtttgga gtggtctatg gaggaaaaca ccggaagaca ggccgggacg 781 tggcagttaa ggtcattgac aaactgcgct tccctaccaa gcaggagagc cagctccgga 841 atgaagtggc cattctgcag agcctgcggc atcccgggat cgtgaacctg gagtgcatgt 901 tcgagacgcc tgagaaagtg tttgtggtga tggagaagct gcatggggac atgttggaga 961 tgatcctgtc cagtgagaag ggccggctgc ctgagcgcct caccaagttc ctcatcaccc 1021 agatcctggt ggctttgaga caccttcact tcaagaacat tgtccactgt gacttgaaac 1081 cagaaaacgt gttgctggca tcagcagacc catttcctca ggtgaagctg tgtgactttg 1141 gctttgctcg catcatcggc gagaagtcgt tccgccgctc agtggtgggc acgccggcct 1201 acctggcacc cgaggtgctg ctcaaccagg gctacaaccg ctcgctggac atgtggtcag 1261 tgggcgtgat catgtacgtc agcctcagcg gcaccttccc tttcaacgag gatgaggaca 1321 tcaatgacca gatccagaac gccgccTTCA TGTACCCGGC CAGCCCCTGG AGCCACATCT 1381 CAGCTGGAGC CATTGACCTC ATCAACAACC TGCTGCAGGT GAAGATGCGC AAACGCTACA 1441 GCGTGGACAA ATCTCTCAGC CACCCCTGGT TACAGGAGTA CCAGACGTGG CTGGACCTCC 1501 GAGAGCTGGA GGGGAAGATG GGAGAGCGAT ACATCACGCA TGAGAGTGAC GACGCGCGCT 1561 GGGAGCAGTT TGCAGCAGAG CATCCGCTGC CTGGGTCTGG GCTGCCCACG GACAGGGATC 1621 TCGGTGGGGC CTGTCCACCA CAGGACCACG ACATGCAGGG GCTGGCGGAG CGCATCAGTG 1681 TTCTCTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1741 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1801 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTATAC TAGACCGTTA TTCTAATGAC 1861 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt