Construct: ORF TRCN0000489174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022008.1_s317c1
- DNA Barcode:
- TCTCCCAGGCCTTAGTTAAAGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDE1B (5153)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489174
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5153 | PDE1B | phosphodiesterase 1B | NM_001288769.1 | 99.9% | 100% | 1353T>C |
| 2 | human | 5153 | PDE1B | phosphodiesterase 1B | XM_017019433.1 | 99.9% | 100% | 1353T>C |
| 3 | human | 5153 | PDE1B | phosphodiesterase 1B | XM_017019432.1 | 98.7% | 98.8% | 1_18del;1371T>C |
| 4 | human | 5153 | PDE1B | phosphodiesterase 1B | NM_001165975.3 | 95.8% | 95.9% | 1_63del;1416T>C |
| 5 | human | 5153 | PDE1B | phosphodiesterase 1B | NM_000924.4 | 92.2% | 92.3% | 1_123del;1476T>C |
| 6 | human | 5153 | PDE1B | phosphodiesterase 1B | XM_011538456.1 | 92.1% | 92.1% | 1_126del;1479T>C |
| 7 | human | 5153 | PDE1B | phosphodiesterase 1B | NM_001288768.1 | 80.5% | 80.6% | 0_1ins288;1065T>C |
| 8 | human | 5153 | PDE1B | phosphodiesterase 1B | NM_001315534.2 | 80.5% | 80.6% | 0_1ins288;1065T>C |
| 9 | human | 5153 | PDE1B | phosphodiesterase 1B | NM_001315535.2 | 67% | 67% | 0_1ins489;864T>C |
| 10 | mouse | 18574 | Pde1b | phosphodiesterase 1B, Ca2+-... | NM_001285890.1 | 86.6% | 91.8% | (many diffs) |
| 11 | mouse | 18574 | Pde1b | phosphodiesterase 1B, Ca2+-... | XM_006520588.3 | 86.6% | 91.8% | (many diffs) |
| 12 | mouse | 18574 | Pde1b | phosphodiesterase 1B, Ca2+-... | NM_008800.2 | 83.5% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1554
- ORF length:
- 1485
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtgaagcag ttggagaatg gggagataaa cattgaggag ctgaagaaaa 121 atctggagta cacagcttct ctgctggaag ccgtctacat agatgagaca cggcaaatct 181 tggacacgga ggacgagctg caggagctgc ggtcagatgc cgtgccttcg gaggtgcggg 241 actggctggc ctccaccttc acccagcagg cccgggccaa aggccgccga gcagaggaga 301 agcccaagtt ccgaagcatt gtgcacgctg tgcaggctgg gatcttcgtg gaacggatgt 361 tccggagaac atacacctct gtgggcccca cttactctac tgcggttctc aactgtctca 421 agaacctgga tctctggtgc tttgatgtct tttccttgaa ccaggcagca gatgaccatg 481 ccctgaggac cattgttttt gagttgctga ctcggcataa cctcatcagc cgcttcaaga 541 ttcccactgt gtttttgatg agtttcctgg atgccttgga gacaggctat gggaagtaca 601 agaatcctta ccacaaccag atccacgcag ccgatgttac ccagacagtc cattgcttct 661 tgctccgcac agggatggtg cactgcctgt cggagattga gctcctggcc atcatctttg 721 ctgcagctat ccatgattat gagcacacgg gcactaccaa cagcttccac atccagacca 781 agtcagaatg tgccatcgtg tacaatgatc gttcagtgct ggagaatcac cacatcagct 841 ctgttttccg attgatgcag gatgatgaga tgaacatttt catcaacctc accaaggatg 901 agtttgtaga actccgagcc ctggtcattg agatggtgtt ggccacagac atgtcctgcc 961 atttccagca agtgaagacc atgaagacag ccttgcaaca gctggagagg attgacaagc 1021 ccaaggccct gtctctactg ctccatgctg ctgacatcag ccacccaacc aagcagtggt 1081 tggtccacag ccgttggacc aaggccctca tggaggaatt cttccgtcag ggtgacaagg 1141 aggcagagtt gggcctgccc ttttctccac tctgtgaccg cacttccact ctagtggcac 1201 agtcTCAGAT AGGGTTCATC GACTTCATTG TGGAGCCCAC ATTCTCTGTG CTGACTGACG 1261 TGGCAGAGAA GAGTGTTCAG CCCCTGGCGG ATGAGGACTC CAAGTCTAAA AACCAGCCCA 1321 GCTTTCAGTG GCGCCAGCCC TCTCTGGATG TGGAAGTGGG AGACCCCAAC CCTGATGTGG 1381 TCAGCTTTCG TTCCACCTGG GTCAAGCGCA TTCAGGAGAA CAAGCAGAAA TGGAAGGAAC 1441 GGGCAGCAAG TGGCATCACC AACCAGATGT CCATTGACGA GCTGTCCCCC TGTGAAGAAG 1501 AGGCCCCCCC ATCCCCTGCC GAAGATGAAC ACAACCAGAA TGGGAATCTG GATTAGGACC 1561 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GATCTCCCAG GCCTTAGTTA AAGTTCACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt