Transcript: Mouse NM_008800.2

Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Pde1b (18574)
Length:
3262
CDS:
353..1960

Additional Resources:

NCBI RefSeq record:
NM_008800.2
NBCI Gene record:
Pde1b (18574)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008800.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437963 GATGTTCCGGAGAACGTATAC pLKO_005 760 CDS 100% 10.800 15.120 N Pde1b n/a
2 TRCN0000434573 GACAGGCTATGGGAAATATAA pLKO_005 985 CDS 100% 15.000 12.000 N Pde1b n/a
3 TRCN0000438413 TGTGATCGCACTTCCACATTG pLKO_005 1577 CDS 100% 10.800 7.560 N Pde1b n/a
4 TRCN0000115005 CAAATCCTAGACACTGAAGAT pLKO.1 578 CDS 100% 4.950 3.465 N Pde1b n/a
5 TRCN0000048974 GCAGGATGATGAGATGAACAT pLKO.1 1261 CDS 100% 4.950 3.465 N PDE1B n/a
6 TRCN0000115001 GCCTCCAAGTTTCTAAGCAAT pLKO.1 2284 3UTR 100% 4.950 3.465 N Pde1b n/a
7 TRCN0000115003 CCTGTCAGAGATTGAGGTCTT pLKO.1 1090 CDS 100% 4.050 2.835 N Pde1b n/a
8 TRCN0000115002 CCTTCTTTAGATGTGGACGTA pLKO.1 1742 CDS 100% 2.640 1.848 N Pde1b n/a
9 TRCN0000115004 CCAGAATGGGAATCTGGATTA pLKO.1 1939 CDS 100% 1.080 0.756 N Pde1b n/a
10 TRCN0000437800 GAGTTGCTGACTCGGCATAAC pLKO_005 905 CDS 100% 10.800 7.560 N PDE1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008800.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491987 GTACAACTGTTGCATTTAAGTCCG pLX_317 21% 90.6% 95.8% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489174 TCTCCCAGGCCTTAGTTAAAGTTC pLX_317 24% 83.5% 88.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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