Construct: ORF TRCN0000489178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021816.1_s317c1
- DNA Barcode:
- AGCGATGGTCATCATCGAACCAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC5A10 (125206)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489178
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_152351.5 | 100% | 100% | |
| 2 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001042450.3 | 97.3% | 97.3% | 979_980ins48 |
| 3 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | XM_017024191.2 | 94.2% | 94.2% | 1289_1399del |
| 4 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001270648.2 | 92.9% | 92.9% | 557_558ins81;898_899ins48 |
| 5 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | XM_011523652.2 | 91.8% | 91.8% | 979_980ins48;1241_1351del |
| 6 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001270649.1 | 91.5% | 91.5% | 981_982ins156 |
| 7 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | XM_011523653.3 | 90.1% | 90.1% | 557_558ins81;1208_1318del |
| 8 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001282417.1 | 81.5% | 81.5% | 0_1ins168;389_390ins81;1040_1150del |
| 9 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | NM_001033227.2 | 81.8% | 86.2% | (many diffs) |
| 10 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531980.3 | 81.8% | 86.2% | (many diffs) |
| 11 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_017314221.1 | 77.4% | 81.6% | (many diffs) |
| 12 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531978.3 | 74.8% | 78.5% | (many diffs) |
| 13 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531979.3 | 73.9% | 78% | (many diffs) |
| 14 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531981.3 | 44.8% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 1914
- ORF length:
- 1836
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcct cgagaccatg gccgccaact ccaccagcga cctccacact cccgggacgc 121 agctgagcgt ggctgacatc atcgtcatca ctgtgtattt tgctctgaat gtggccgtgg 181 gcatatggtc ctcttgtcgg gccagtagga acacggtgaa tggctacttc ctggcaggcc 241 gggacatgac gtggtggccg attggagcct ccctcttcgc cagcagcgag ggctctggcc 301 tcttcattgg actggcgggc tcaggcgcgg caggaggtct ggccgtggca ggcttcgagt 361 ggaatgccac gtacgtgctg ctggcactgg catgggtgtt cgtgcccatc tacatctcct 421 cagagatcgt caccttacct gagtacattc agaagcgcta cgggggccag cggatccgca 481 tgtacctgtc tgtcctgtcc ctgctactgt ctgtcttcac caagatatcg ctggacctgt 541 acgcgggggc tctgtttgtg cacatctgcc tgggctggaa cttctacctc tccaccatcc 601 tcacgctcgg catcacagcc ctgtacacca tcgcaggggg cctggctgct gtaatctaca 661 cggacgccct gcagacgctc atcatggtgg tgggggctgt catcctgaca atcaaagctt 721 ttgaccagat cggtggttac gggcagctgg aggcagccta cgcccaggcc attccctcca 781 ggaccattgc caacaccacc tgccacctgc cacgtacaga cgccatgcac atgtttcgag 841 acccccacac aggggacctg ccgtggaccg ggatgacctt tggcctgacc atcatggcca 901 cctggtactg gtgcaccgac caggtcatcg tgcagcgatc actgtcagcc cgggacctga 961 accatgccaa ggcgggctcc atcctggcca gctacctcaa gatgctcccc atgggcctga 1021 tcatcatgcc gggcatgatc agccgcgcat tgttcccagg tgctcatgtc tatgaggaga 1081 gacaccaagt gtccgtctct cgaacagatg atgtgggctg cgtggtgccg tccgagtgcc 1141 tgcgggcctg cggggccgag gtcggctgct ccaacatcgc ctaccccaag ctggtcatgg 1201 aactgatgcc catcggtctg cgggggctga tgatcgcagt gatgctggcg gcgctcatgt 1261 cgtcgctgac ctccatcttc aacagcagca gcaccctctt cactatggac atctggaggc 1321 ggctgcgtcc ccgctccggc gagcgggagc tcctgctggt gggacggctg gtcatagtgg 1381 cactcatcgg cgtgagtgtg gcctggatcc ccgtcctgca ggactccaac agcgggcaac 1441 tcttcatcta catgcagtca gtgaccagct ccctggcccc accagtgact gcagtctttg 1501 tcctgggcgt cttctggcga cgtgccaacg agcagggggc cTTCTGGGGC CTGATAGCAG 1561 GGCTGGTGGT GGGGGCCACG AGGCTGGTCC TGGAATTCCT GAACCCAGCC CCACCGTGCG 1621 GAGAGCCAGA CACGCGGCCA GCCGTCCTGG GGAGCATCCA CTACCTGCAC TTCGCTGTCG 1681 CCCTCTTTGC ACTCAGTGGT GCTGTTGTGG TGGCTGGAAG CCTGCTGACC CCACCCCCAC 1741 AGAGTGTCCA GATTGAGAAC CTTACCTGGT GGACCCTGGC TCAGGATGTG CCCTTGGGAA 1801 CTAAAGCAGG TGATGGCCAA ACACCCCAGA AACACGCCTT CTGGGCCCGT GTCTGTGGCT 1861 TCAATGCCAT CCTCCTCATG TGTGTCAACA TATTCTTTTA TGCCTACTTC GCCTGAAGAT 1921 CTGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGAAG CGATGGTCAT CATCGAACCA GTACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt