Construct: ORF TRCN0000489191

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020142.1_s317c1
DNA Barcode:
CTGATGCCGCCGCGGCGTTTTATT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
AURKB (9212)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489191
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9212 AURKB aurora kinase B NM_001313950.2 99.8% 99.7% 885C>T;893T>C
2 human 9212 AURKB aurora kinase B NM_004217.4 99.8% 99.7% 885C>T;893T>C
3 human 9212 AURKB aurora kinase B NM_001284526.2 99.5% 99.4% 207_209delCAG;888C>T;896T>C
4 human 9212 AURKB aurora kinase B NM_001313953.3 88.7% 86.9% (many diffs)
5 human 9212 AURKB aurora kinase B NM_001256834.3 87.8% 87.7% 0_1ins123;762C>T;770T>C
6 human 9212 AURKB aurora kinase B NM_001313951.1 87.8% 87.7% 0_1ins123;762C>T;770T>C
7 human 9212 AURKB aurora kinase B XM_011524072.3 87.8% 87.7% 0_1ins123;762C>T;770T>C
8 human 9212 AURKB aurora kinase B XM_017025307.2 87.8% 87.7% 0_1ins123;762C>T;770T>C
9 human 9212 AURKB aurora kinase B NM_001313952.2 87.6% 87.5% (many diffs)
10 human 9212 AURKB aurora kinase B XM_017025308.2 76.9% 75% (many diffs)
11 human 9212 AURKB aurora kinase B NR_132731.1 74.4% (many diffs)
12 human 9212 AURKB aurora kinase B NR_132730.2 71.4% (many diffs)
13 human 9212 AURKB aurora kinase B NM_001313954.2 54.1% 48.9% (many diffs)
14 human 9212 AURKB aurora kinase B XM_017025309.1 54.1% 48.9% (many diffs)
15 human 9212 AURKB aurora kinase B XM_017025310.1 54.1% 48.9% (many diffs)
16 human 9212 AURKB aurora kinase B XM_017025311.1 54.1% 48.9% (many diffs)
17 human 9212 AURKB aurora kinase B NM_001313955.2 50% 47.2% (many diffs)
18 mouse 20877 Aurkb aurora kinase B NM_011496.2 81.4% 82.9% (many diffs)
19 mouse 20877 Aurkb aurora kinase B XM_006532725.3 53.7% 55% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1104
ORF length:
1032
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcccag aaggagaact cctacccctg gccctacggc cgacagacgg
121ctccatctgg cctgagcacc ctgccccagc gagtcctccg gaaagagcct gtcaccccat
181ctgcacttgt cctcatgagc cgctccaatg tccagcccac agctgcccct ggccagaagg
241tgatggagaa tagcagtggg acacccgaca tcttaacgcg gcacttcaca attgatgact
301ttgagattgg gcgtcctctg ggcaaaggca agtttggaaa cgtgtacttg gctcgggaga
361agaaaagcca tttcatcgtg gcgctcaagg tcctcttcaa gtcccagata gagaaggagg
421gcgtggagca tcagctgcgc agagagatcg aaatccaggc ccacctgcac catcccaaca
481tcctgcgtct ctacaactat ttttatgacc ggaggaggat ctacttgatt ctagagtatg
541ccccccgcgg ggagctctac aaggagctgc agaagagctg cacatttgac gagcagcgaa
601cagccacgat catggaggag ttggcagatg ctctaatgta ctgccatggg aagaaggtga
661ttcacagaga cataaagcca gaaaatctgc tcttagggct caagggagag ctgaagattg
721ctgacttcgg ctggtctgtg catgcgccct cccTGAGGAG GAAGACAATG TGTGGCACCC
781TGGACTACCT GCCCCCAGAG ATGATTGAGG GGCGCATGCA CAATGAGAAG GTGGATCTGT
841GGTGCATTGG AGTGCTTTGC TATGAGCTGC TGGTGGGGAA CCCACCCTTT GAGAGTGCAT
901CACACAACGA GACCTATCGC CGCATCGTCA AGGTGGACCT AAAGTTCCCC GCTTCTGTGC
961CCACGGGAGC CCAGGACCTC ATCTCCAAAC TGCTCAGGCA TAACCCCTCG GAACGGCTGC
1021CCCTGGCCCA GGTCTCAGCC CACCCTTGGG TCCGGGCCAA CTCTCGGAGG GTGCTGCCTC
1081CCTCTGCCCT TCAATCTGTC GCCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1141GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1201AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTGATGCC
1261GCCGCGGCGT TTTATTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1321aagatt

Download FASTA (ORF) (Full)

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