Construct: ORF TRCN0000489195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019285.1_s317c1
- DNA Barcode:
- AATCCACCTGTACGCTCATGCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRG2 (10149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489195
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001079859.3 | 99.9% | 99.9% | 1248T>C;3009_3010insG |
| 2 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001079860.3 | 99.1% | 99.1% | 201_202ins24;1224T>C;2985_2986insG |
| 3 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001079858.3 | 98.5% | 98.5% | 260_301del;1290T>C;3051_3052insG |
| 4 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | XM_006724455.3 | 98.5% | 98.5% | 260_301del;1290T>C;3051_3052insG |
| 5 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | XM_011545434.1 | 98.5% | 98.5% | 260_301del;1290T>C;3051_3052insG |
| 6 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | XM_011545435.2 | 98.5% | 98.5% | 260_301del;1290T>C;3051_3052insG |
| 7 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001184837.2 | 98.3% | 98.3% | 153_154ins48;1200T>C;2961_2962insG |
| 8 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_005756.4 | 98.2% | 98.2% | (many diffs) |
| 9 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001184835.2 | 97.5% | 97.5% | 152_153ins72;1176T>C;2937_2938insG |
| 10 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001184833.1 | 96.9% | 96.9% | (many diffs) |
| 11 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001184836.1 | 96.1% | 96.1% | (many diffs) |
| 12 | human | 10149 | ADGRG2 | adhesion G protein-coupled ... | NM_001184834.2 | 93.5% | 93.5% | (many diffs) |
| 13 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | NM_001079857.2 | 82.9% | 80.4% | (many diffs) |
| 14 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | NM_001079847.2 | 82.7% | 80.1% | (many diffs) |
| 15 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | NM_178712.4 | 82.5% | 79.9% | (many diffs) |
| 16 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | NM_001290445.1 | 82.2% | 79.6% | (many diffs) |
| 17 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528824.3 | 81.9% | 79.4% | (many diffs) |
| 18 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_011247817.2 | 81.9% | 79.4% | (many diffs) |
| 19 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_017318479.1 | 81.9% | 79.4% | (many diffs) |
| 20 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | NM_001079848.2 | 81.5% | 79.1% | (many diffs) |
| 21 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528829.2 | 80.9% | 78.5% | (many diffs) |
| 22 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528812.2 | 77.2% | 74.7% | (many diffs) |
| 23 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528813.2 | 76.9% | 74.5% | (many diffs) |
| 24 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528808.2 | 76.8% | 74.4% | (many diffs) |
| 25 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528810.2 | 76.7% | 74.3% | (many diffs) |
| 26 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528811.2 | 76.4% | 74% | (many diffs) |
| 27 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528806.2 | 76.3% | 73.9% | (many diffs) |
| 28 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528816.2 | 75.8% | 73.5% | (many diffs) |
| 29 | mouse | 237175 | Adgrg2 | adhesion G protein-coupled ... | XM_006528819.2 | 75% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3087
- ORF length:
- 3012
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggtt ttctctgtca ggcagtgtgg ccatgttggc agaactgaag 121 aagttttact gacgttcaag atattccttg tcatcatttg tcttcatgtc gttctggtaa 181 catccctgga agaagatact gataattcca gtttgtcacc accacctgct aaattatctg 241 ttgtcagttt tgccccctcc tccaatggta ctccagaggt tgaaacaaca agcctcaatg 301 atgttacttt aagcttactc ccttcaaacg aaacaggcgt caaaccccag agaaatatct 361 gcaatttgtc atctatttgc aatgactcag cattttttag aggtgagatc atgtttcaat 421 atgataaaga aagcactgtt ccccagaatc aacatataac gaatggcacc ttaactggag 481 tcctgtctct aagtgaatta aaacgctcag agctcaacaa aaccctgcaa accctaagtg 541 agacttactt tataatgtgt gctacagcag aggcccaaag cacattaaat tgtacattca 601 caataaaact gaataataca atgaatgcat gtgctgtaat agctgctttg gaaagagtaa 661 agattcgacc aatggaacac tgctgctgtt ctgtcaggat accctgccct tcctccccag 721 aagagttgga aaagcttcag tgtgacctgc aggatcccat tgtctgtctt gctgaccatc 781 cacgtggccc accattttct tccagccaat ccatcccagt ggtgcctcgg gccactgtgc 841 tttcccaggt ccccaaagct acctcttttg ctgagcctcc agattattca cctgtgaccc 901 acaatgttcc ctctccaata ggggagattc aacccctttc accccagcct tcagctccca 961 tagcttccag ccctgccatt gacatgcccc cacagtctga aacgatctct tcccctatgc 1021 cccaaaccca tgtctccggc accccacctc ctgtgaaagc ctcattttcc tctcccaccg 1081 tgtctgcccc tgcgaatgtc aacactacca gcgcacctcc tgtccagaca gacatcgtca 1141 acaccagcag tatttctgat cttgagaacc aagtgttgca gatggagaag gctctgtcct 1201 tgggcagcct ggagcctaac ctcgcaggag aaatgatcaa ccaagtcagc agactccttc 1261 attccccgcc tgacatgctg gcccctctgg ctcaaagatt gctgaaagta gtggatgaca 1321 tcggcctaca gctgaacttt tcaaacacga ctataagtct aacctcccct tctttggctc 1381 tggctgtgat cagagtgaat gccagtagtt tcaacacaac tacctttgtg gcccaagacc 1441 ctgcaaatct tcaggtttct ctggaaaccc aagctcctga gaacagtatt ggcacaatta 1501 ctcttccttc atcgctgatg aataatttac cagctcatga catggagcta gcttccaggg 1561 ttcagttcaa tttttttgaa acacctgctt tgtttcagga tccttccctg gagaacctct 1621 ctctgatcag ctacgtcata tcatcgagtg ttgcaaacct gaccgtcagg aacttgacaa 1681 gaaacgtgac agtcacatta aagcacatca acccgagcca ggatgagtta acagtgagat 1741 gtgtattttg ggacttgggc agaaatggtg gcagaggagg ctggtcagac aatggctgct 1801 ctgtcaaaga caggagattg aatgaaacca tctgtacctg tagccatcta acaagcttcg 1861 gcgttctgct ggacctatct aggacatctg tgctgcctgc tcaaatgatg gctctgacgt 1921 tcattacata tattggttgt gggctttcat caatttttct gtcagtgact cttgtaacct 1981 acatagcttt tgaaaagatc cggagggatt acccttccaa aatcctcatc cagctgtgtg 2041 ctgctctgct tctgctgaac ctggtcttcc tcctggactc gtggattgct ctgtataaga 2101 tgcaaggcct ctgcatctca gtggctgtat ttcttcatta ttttctcttg gtctcattca 2161 catggatggg cctagaagca ttccatatgt acctggccct tgtcaaagta tttaatactt 2221 acatccgaaa atacatcctt aaattctgca ttgtcggttg gggggtacca gctgtggttg 2281 tgaccatcat cctgactata tccccagata actatgggct tggatcctat gggaaattcc 2341 ccaatggttc accggatgac ttctgctgga tcaacaacaa tgcagtattc tacattacgg 2401 tggtgggata tttctgtgtg atatttttgc tgaacgtcag catgttcatt gtggtcctgg 2461 ttcagctctg tcgaattaaa aagaagaagc aactgggagc ccagcgaaaa accagtattc 2521 aagacctcag gagtatcgct ggccttacat ttttactggg aataacttgg ggctttgcct 2581 tctttgcctg gggaccagtt aacgtgacct tcatgtatct gtttgccatc tttaatacct 2641 tacaaggatt tttcatattc atcttttact gtgtggccaa agaaaatgtc aggaagcaat 2701 ggaggcggta tctttgttgt gGAAAGTTAC GGCTGGCTGA AAATTCTGAC TGGAGTAAAA 2761 CTGCTACTAA TGGTTTAAAG AAGCAGACTG TAAACCAAGG AGTGTCCAGC TCTTCAAATT 2821 CCTTACAGTC AAGCAGTAAC TCCACTAACT CCACCACACT GCTAGTGAAT AATGATTGCT 2881 CAGTACACGC AAGCGGGAAT GGAAATGCTT CTACAGAGAG GAATGGGGTC TCTTTTAGTG 2941 TTCAGAATGG AGATGTGTGC CTTCACGATT TCACTGGAAA ACAGCACATG TTTAACGAGA 3001 AGGAAGATTC CTGCAATGGG AAAGGCCGTA TGGCTCTCAG AAGGACTTCA AAGCGGGGAA 3061 GCTTACACTT TATTGAGCAA ATGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3121 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3181 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATCCACCTGT 3241 ACGCTCATGC CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3301 att