Transcript: Mouse NM_001079848.2

Mus musculus adhesion G protein-coupled receptor G2 (Adgrg2), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Adgrg2 (237175)
Length:
4642
CDS:
107..3055

Additional Resources:

NCBI RefSeq record:
NM_001079848.2
NBCI Gene record:
Adgrg2 (237175)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001079848.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414292 GTTCAGCTCTGTCGAATTAAA pLKO_005 2429 CDS 100% 15.000 21.000 N Adgrg2 n/a
2 TRCN0000423479 TATCACGGTTGTGGGATATTT pLKO_005 2362 CDS 100% 15.000 21.000 N Adgrg2 n/a
3 TRCN0000221854 GCCATCTTTAACACCTTACAA pLKO.1 2594 CDS 100% 5.625 7.875 N Adgrg2 n/a
4 TRCN0000413479 TTCAGTCTGTTGGTAAGTTTA pLKO_005 3454 3UTR 100% 13.200 10.560 N Adgrg2 n/a
5 TRCN0000436715 CTGATGGCTGTTCCGTCAAAG pLKO_005 1758 CDS 100% 10.800 8.640 N Adgrg2 n/a
6 TRCN0000221853 GCTGTATAATACCCGAGGTTT pLKO.1 2059 CDS 100% 4.950 3.960 N Adgrg2 n/a
7 TRCN0000367776 GGTTCAGCTCTGTCGAATTAA pLKO_005 2428 CDS 100% 15.000 10.500 N ADGRG2 n/a
8 TRCN0000221852 CCTCCTTTAGAAGATCATATA pLKO.1 3643 3UTR 100% 13.200 9.240 N Adgrg2 n/a
9 TRCN0000221855 GCTCTGACATTTATCACGTAT pLKO.1 1880 CDS 100% 4.950 3.465 N Adgrg2 n/a
10 TRCN0000221856 GCTTACACTTTATCGAGCAAA pLKO.1 3030 CDS 100% 4.950 3.465 N Adgrg2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001079848.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489195 AATCCACCTGTACGCTCATGCCCC pLX_317 12.2% 81.5% 79.1% V5 (many diffs) n/a
2 TRCN0000489489 GGGATAACATGATGAAAACTTGCC pLX_317 11.9% 81.5% 79.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_11460 pDONR223 100% 77.6% 75.5% None (many diffs) n/a
4 ccsbBroad304_11460 pLX_304 0% 77.6% 75.5% V5 (many diffs) n/a
5 TRCN0000474072 TGACGGACGTTTTGGCGTTTTTCG pLX_317 20.1% 77.6% 75.5% V5 (many diffs) n/a
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