Construct: ORF TRCN0000489227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019916.1_s317c1
- DNA Barcode:
- CAGGGGTGGAGGGGATATCAACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAP (2191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2191 | FAP | fibroblast activation prote... | NM_004460.5 | 99.9% | 99.8% | 2280_2281insG |
| 2 | human | 2191 | FAP | fibroblast activation prote... | XM_011510796.3 | 97.4% | 96.3% | (many diffs) |
| 3 | human | 2191 | FAP | fibroblast activation prote... | NM_001291807.3 | 96.6% | 96.5% | 284_285ins75;2205_2206insG |
| 4 | human | 2191 | FAP | fibroblast activation prote... | XR_922891.2 | 70.9% | (many diffs) | |
| 5 | human | 2191 | FAP | fibroblast activation prote... | XM_011510797.3 | 64.3% | 62.9% | (many diffs) |
| 6 | human | 2191 | FAP | fibroblast activation prote... | XM_017003585.2 | 61.9% | 61.5% | (many diffs) |
| 7 | human | 2191 | FAP | fibroblast activation prote... | XR_001738668.2 | 56.9% | 1_145del;1548_1667del;1715_1716ins831 | |
| 8 | mouse | 14089 | Fap | fibroblast activation protein | NM_007986.3 | 89.4% | 89.3% | (many diffs) |
| 9 | mouse | 14089 | Fap | fibroblast activation protein | XM_006498746.3 | 89.4% | 89.3% | (many diffs) |
| 10 | mouse | 14089 | Fap | fibroblast activation protein | XM_017315507.1 | 88.4% | 88.3% | (many diffs) |
| 11 | mouse | 14089 | Fap | fibroblast activation protein | XM_011239289.1 | 76.7% | 74.4% | (many diffs) |
| 12 | mouse | 14089 | Fap | fibroblast activation protein | XM_017315512.1 | 76.7% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2352
- ORF length:
- 2283
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaagacttgg gtaaaaatcg tatttggagt tgccacctct gctgtgcttg 121 ccttattggt gatgtgcatt gtcttacgcc cttcaagagt tcataactct gaagaaaata 181 caatgagagc actcacactg aaggatattt taaatggaac attttcttat aaaacatttt 241 ttccaaactg gatttcagga caagaatatc ttcatcaatc tgcagataac aatatagtac 301 tttataatat tgaaacagga caatcatata ccattttgag taatagaacc atgaaaagtg 361 tgaatgcttc aaattacggc ttatcacctg atcggcaatt tgtatatcta gaaagtgatt 421 attcaaagct ttggagatac tcttacacag caacatatta catctatgac cttagcaatg 481 gagaatttgt aagaggaaat gagcttcctc gtccaattca gtatttatgc tggtcgcctg 541 ttgggagtaa attagcatat gtctatcaaa acaatatcta tttgaaacaa agaccaggag 601 atccaccttt tcaaataaca tttaatggaa gagaaaataa aatatttaat ggaatcccag 661 actgggttta tgaagaggaa atgcttgcta caaaatatgc tctctggtgg tctcctaatg 721 gaaaattttt ggcatatgcg gaatttaatg atacggatat accagttatt gcctattcct 781 attatggcga tgaacaatat cctagaacaa taaatattcc atacccaaag gctggagcta 841 agaatcccgt tgttcggata tttattatcg ataccactta ccctgcgtat gtaggtcccc 901 aggaagtgcc tgttccagca atgatagcct caagtgatta ttatttcagt tggctcacgt 961 gggttactga tgaacgagta tgtttgcagt ggctaaaaag agtccagaat gtttcggtcc 1021 tgtctatatg tgacttcagg gaagactggc agacatggga ttgtccaaag acccaggagc 1081 atatagaaga aagcagaact ggatgggctg gtggattctt tgtttcaaca ccagttttca 1141 gctatgatgc catttcgtac tacaaaatat ttagtgacaa ggatggctac aaacatattc 1201 actatatcaa agacactgtg gaaaatgcta ttcaaattac aagtggcaag tgggaggcca 1261 taaatatatt cagagtaaca caggattcac tgttttattc tagcaatgaa tttgaagaat 1321 accctggaag aagaaacatc tacagaatta gcattggaag ctatcctcca agcaagaagt 1381 gtgttacttg ccatctaagg aaagaaaggt gccaatatta cacagcaagt ttcagcgact 1441 acgccaagta ctatgcactt gtctgctacg gcccaggcat ccccatttcc acccttcatg 1501 atggacgcac tgatcaagaa attaaaatcc tggaagaaaa caaggaattg gaaaatgctt 1561 tgaaaaatat ccagctgcct aaagaggaaa ttaagaaact tgaagtagat gaaattactt 1621 tatggtacaa gatgattctt cctcctcaat ttgacagatc aaagaagtat cccttgctaa 1681 ttcaagtgta tggtggtccc tgcagtcaga gtgtaaggtc tgtatttgct gttaattgga 1741 tatcttatct tgcaagtaag gaagggatgg tcattgcctt ggtggatggt cgaggaacag 1801 ctttccaagg tgacaaactc ctctatgcag tgtatcgaaa gctgggtgtt tatgaagttg 1861 aagaccagat tacagctgtc agaaaattca tagaaatggg tttcattgat gaaaaaagaa 1921 tagccatatg gggctggtcc tatggaggat acgtttcatc actggccctt gcaTCTGGAA 1981 CTGGTCTTTT CAAATGTGGT ATAGCAGTGG CTCCAGTCTC CAGCTGGGAA TATTACGCGT 2041 CTGTCTACAC AGAGAGATTC ATGGGTCTCC CAACAAAGGA TGATAATCTT GAGCACTATA 2101 AGAATTCAAC TGTGATGGCA AGAGCAGAAT ATTTCAGAAA TGTAGACTAT CTTCTCATCC 2161 ACGGAACAGC AGATGATAAT GTGCACTTTC AAAACTCAGC ACAGATTGCT AAAGCTCTGG 2221 TTAATGCACA AGTGGATTTC CAGGCAATGT GGTACTCTGA CCAGAACCAC GGCTTATCCG 2281 GCCTGTCCAC GAACCACTTA TACACCCACA TGACCCACTT CCTAAAGCAG TGTTTCTCTT 2341 TGTCAGACGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2401 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2461 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAGGGG TGGAGGGGAT ATCAACATAC 2521 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt