Construct: ORF TRCN0000489234
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021797.1_s317c1
- DNA Barcode:
- AACTCGAACCGCAACTACAAGGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NUMB (8650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489234
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005743.1 | 100% | 100% | |
| 2 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_003744.5 | 98.3% | 98.3% | 201_202ins33 |
| 3 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005744.1 | 92.6% | 92.6% | 1095_1096ins144 |
| 4 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001320114.1 | 92.6% | 92.6% | 1095_1096ins144 |
| 5 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005745.1 | 90.9% | 90.9% | 201_202ins33;1062_1063ins144 |
| 6 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001136075.2 | 89.3% | 93.4% | (many diffs) |
| 7 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515576.1 | 89.3% | 93.4% | (many diffs) |
| 8 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515577.2 | 89.3% | 93.4% | (many diffs) |
| 9 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_011244012.2 | 89.3% | 93.4% | (many diffs) |
| 10 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272055.1 | 87.6% | 91.7% | (many diffs) |
| 11 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515578.1 | 87.6% | 91.7% | (many diffs) |
| 12 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_010949.2 | 82.7% | 87.2% | (many diffs) |
| 13 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272056.1 | 81% | 85.5% | (many diffs) |
| 14 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515572.2 | 78.8% | 82.4% | (many diffs) |
| 15 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515573.2 | 77.3% | 80.9% | (many diffs) |
| 16 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515574.2 | 73% | 76.9% | (many diffs) |
| 17 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515575.2 | 71.5% | 75.5% | (many diffs) |
| 18 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073564.1 | 43.9% | (many diffs) | |
| 19 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073563.1 | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2025
- ORF length:
- 1953
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttaac catgaacaaa ttacggcaaa gttttaggag aaagaaggat gtttatgttc 121 cagaggccag tcgtccacat cagtggcaga cagatgaaga aggcgttcgc accggaaaat 181 gtagcttccc ggttaagtac cttggccatg tagaagttga tgaatcaaga ggaatgcaca 241 tctgtgaaga tgctgtaaaa agattgaaag ctgaaaggaa gttcttcaaa ggcttctttg 301 gaaaaactgg aaagaaagca gttaaagcag ttctgtgggt ctcagcagat ggactcagag 361 ttgtggatga aaaaactaag gacctcatag ttgaccagac gatagagaaa gtttctttct 421 gtgccccaga caggaacttt gatagagcct tttcttacat atgccgtgat ggcaccactc 481 gtcgctggat ctgtcactgc ttcatggctg tcaaggacac aggtgaaagg ttgagccatg 541 cagtaggctg tgcttttgca gcctgtttag agcgcaagca gaagcgggag aaggaatgtg 601 gagtgactgc tacttttgat gctagtcgga ccacttttac aagagaagga tcattccgtg 661 tcacaacagc cactgaacaa gcagaaagag aggagatcat gaaacaaatg caagatgcca 721 agaaagctga aacagataag atagtcgttg gttcatcagt tgcccctggc aacactgccc 781 catccccatc ctctcccacc tctcctactt ctgatgccac gacctctctg gagatgaaca 841 atcctcatgc catcccacgc cggcatgctc caattgaaca gcttgctcgc caaggctctt 901 tccgaggttt tcctgctctt agccagaaga tgtcaccctt taaacgccaa ctatccctac 961 gcatcaatga gttgccttcc actatgcaga ggaagactga tttccccatt aaaaatgcag 1021 tgccagaagt agaaggggag gcagagagca tcagctccct gtgctcacag atcaccaatg 1081 ccttcagcac acctgaggac cccttctcat ctgctccgat gaccaaacca gtgacagtgg 1141 tggcaccaca atctcctacc ttccaagcta atggcactga ctcagccttc catgtgcttg 1201 ctaagccagc ccatactgct ctagcacccg tagcaatgcc tgtgcgtgaa accaaccctt 1261 gggcccatgc ccctgatgct gctaacaagg aaattgcagc cacatgttcg gggaccgagt 1321 ggggtcaatc ttctggtgct gcctctccag gtctcttcca ggccggtcat agacgtactc 1381 cctctgaggc cgaccgatgg ttagaagagg tgtctaagag cgtccgggct cagcagcccc 1441 aggcctcagc tgctcctctg cagccagttc tccagcctcc tccacccact gccatctccc 1501 agccagcatc acctttccaa gggaatgcat tcctcacctc tcagcctgtg ccagtgggtg 1561 tggtcccagc cctgcaacca gcctttgtcc ctgcccagtc ctatcctgtg gccaatggaa 1621 tgccctatcc agcccctaat gtgcctgtgg tgggcatcac tcccTCCCAG ATGGTGGCCA 1681 ACGTATTTGG CACTGCAGGC CACCCTCAGG CTGCCCATCC CCATCAGTCA CCCAGCCTGG 1741 TCAGGCAGCA GACATTCCCT CACTACGAGG CAAGCAGTGC TACCACCAGT CCCTTCTTTA 1801 AGCCTCCTGC TCAGCACCTC AACGGTTCTG CAGCTTTCAA TGGTGTAGAT GATGGCAGGT 1861 TGGCCTCAGC AGACAGGCAT ACAGAGGTTC CTACAGGCAC CTGCCCAGTG GATCCTTTTG 1921 AAGCCCAGTG GGCTGCATTA GAAAATAAGT CCAAGCAGCG TACTAATCCC TCCCCTACCA 1981 ACCCTTTCTC CAGTGACTTA CAGAAGACGT TTGAAATTGA ACTTTAAGAC CCAGCTTTCT 2041 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2101 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2161 GTGGAAAGGA CGAAACTCGA ACCGCAACTA CAAGGAAACG CGTTAAGTCg acaatcaacc 2221 tctggattac aaaatttgtg aaagatt