Construct: ORF TRCN0000489245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020513.1_s317c1
- DNA Barcode:
- CTGCCTTATAAACTAAGGTCTGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LRRK1 (79705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_005254979.3 | 99.9% | 100% | 1332A>G |
| 2 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522017.2 | 99.9% | 100% | 1332A>G |
| 3 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522018.2 | 99.9% | 100% | 1332A>G |
| 4 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_017022570.1 | 85.9% | 85.9% | 1_642del;1974A>G |
| 5 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522015.3 | 74.1% | 74.1% | 1_1371del;2703A>G |
| 6 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522016.2 | 68.4% | 68.4% | 1_1812del;3144A>G |
| 7 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | NM_024652.6 | 64.9% | 65% | 1_2115del;3447A>G |
| 8 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522012.2 | 64.9% | 65% | 1_2115del;3447A>G |
| 9 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522013.2 | 64.9% | 65% | 1_2115del;3447A>G |
| 10 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522014.2 | 63.3% | 63.3% | 1_2115del;3447A>G;5767_5768ins102 |
| 11 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XR_931905.2 | 42.5% | (many diffs) | |
| 12 | human | 79705 | LRRK1 | leucine rich repeat kinase 1 | XM_011522019.2 | 18.9% | 17.5% | 1_2115del;3132_3133ins164;3258_3259ins2623 |
| 13 | mouse | 233328 | Lrrk1 | leucine-rich repeat kinase 1 | XM_006540841.1 | 86.5% | 91% | (many diffs) |
| 14 | mouse | 233328 | Lrrk1 | leucine-rich repeat kinase 1 | XM_006540840.3 | 76.7% | 80.7% | (many diffs) |
| 15 | mouse | 233328 | Lrrk1 | leucine-rich repeat kinase 1 | XM_006540839.3 | 56.7% | 59.7% | (many diffs) |
| 16 | mouse | 233328 | Lrrk1 | leucine-rich repeat kinase 1 | NM_146191.3 | 56.3% | 59.2% | (many diffs) |
| 17 | mouse | 233328 | Lrrk1 | leucine-rich repeat kinase 1 | XM_006540838.3 | 56.2% | 59.1% | (many diffs) |
| 18 | mouse | 233328 | Lrrk1 | leucine-rich repeat kinase 1 | XR_391340.3 | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4002
- ORF length:
- 3930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccact gtcaaccagt gcttcttcac ggacaaggcc ctgtacgtgg 121 tggtctggaa cctggcgctg ggggaggagg ccgtggccaa cctccagttc tggctgctca 181 acatcgaggc caaggcccca aacgccgtgg tgctggtggt cgggacgcac ctggatttaa 241 ttgaagccaa gttccgtgtg gaaaggattg caacgctgcg tgcctatgtg ctggcactct 301 gccgctcccc ctccggctcc agggccacag gcttcccaga catcaccttc aaacacttac 361 atgagatttc ctgcaagagc ctggaaggtc aggaagggct gcgacagctg attttccacg 421 tcacgtgcag catgaaggac gtgggcagca ccatcggctg ccagcgactg gcagggcggc 481 tgatccccag gagctacctg agcctgcagg aggccgtgct ggcagagcag cagcgccgca 541 gccgggacga cgacgtgcag tacctgacgg acaggcagct ggagcagctg gtggagcaga 601 cgcccgacaa cgacatcaag gactacgagg acctgcagtc agccatcagc ttcctcatag 661 aaaccggcac cctgctccat ttcccggaca ccagccacgg cctgaggaac ctctacttcc 721 tcgaccctat ttggctctcc gaatgtctgc agaggatctt taatattaag ggctctcggt 781 cagtggccaa gaatggggtg atcagagcag aagacctcag gatgctgctg gtggggactg 841 gcttcacgca gcagacggaa gagcagtact tccagttcct ggccaagttt gagatcgccc 901 tgcccgtcgc caatgacagc tacctcctgc cccatctcct tccatctaaa cctggcctgg 961 acacccacgg tatgcggcac cccacagcca acaccattca gagggtattt aagatgagct 1021 tcgttcccgt tggcttctgg caaaggttta tagcacggat gctgatcagc ctggcggaga 1081 tggacctgca gctttttgaa aacaagaaga atactaaaag caggaacagg aaagtcacca 1141 tttacagttt tacaggaaac cagagaaatc gctgtagcac attcagagtg aaaagaaatc 1201 agaccatcta ttggcaggaa gggctcctgg tcacttttga tgggggctac ctcagtgtgg 1261 aatcttccga cgtgaactgg aaaaagaaga aaagcggagg aatgaaaatt gtttgccaat 1321 cagaagtgag ggacttctca gccatggctt tcatcacgga ccacgtcaat tccttgattg 1381 atcagtggtt tcccgccctg acggccacag agagcgacgg gacgccactc atggagcagt 1441 acgtgccctg cccggtctgc gagacagcct gggcccagca cacggacccc agtgagaaat 1501 cagaggatgt gcagtacttc gacatggaag actgtgtcct gacggccatc gagcgggact 1561 tcatctcctg ccccagacac ccggacctcc ccgtgccgct gcaggagctg gtccctgaac 1621 tgttcatgac cgacttcccg gccaggctct tcctggagaa cagcaagctg gagcacagcg 1681 aggacgaggg cagcgtcctg ggccagggcg gcagtggcac cgtcatctac cgggcccggt 1741 accagggcca gcctgtggcc gtcaagcgct tccacatcaa aaaattcaag aactttgcta 1801 acgtaccggc agacaccatg ctgaggcacc tgcgggccac cgatgccatg aagaacttct 1861 ccgagttccg gcaggaggcc agcatgctgc acgcgctgca gcacccctgc atcgtggcgc 1921 tcatcggcat cagcatccac ccgctctgct tcgccctgga gctcgcgccg ctcagcagcc 1981 tcaacaccgt gctgtccgag aacgccagag attcttcctt tatacccctg ggacacatgc 2041 tcacccaaaa aatagcctac cagatcgcct cgggcctggc ctacctgcac aagaaaaaca 2101 tcatcttctg tgacctgaag tcggacaaca ttctggtgtg gtcccttgac gtcaaggagc 2161 acatcaacat caagctatct gactacggga tttcgaggca gtcattccat gagggcgccc 2221 taggcgtgga gggcactcct ggctaccagg ccccagagat caggcctcgc attgtatatg 2281 atgagaaggt agatatgttc tcctatggaa tggtgctcta cgagttgctg tcaggacagc 2341 gccctgcact gggccaccac cagctccaga ttgccaagaa gctgtccaag ggcatccgcc 2401 cggttctggg gcagccggag gaagtgcagt tccggcgact gcaggcgctc atgatggagt 2461 gctgggacac taagccagag aagcgaccgc tggccctgtc ggtggtgagc cagatgaagg 2521 acccgacttt tgccaccttc atgtatgaac tgtgctgtgg gaagcagaca gccttcttct 2581 catcccaggg ccaggagtac accgtggtgt tttgggatgg aaaagaggag tccaggaact 2641 acacggtggt gaacacagag aagggcctca tggaggtgca gaggatgtgc tgccctggga 2701 tgaaggtgag ctgccagctc caggtccaga gatccctgtg gacagccacc gaggaccaga 2761 aaatctacat ctacaccctc aagggcatgt gccccttaaa cacaccccaa caggccttgg 2821 atactccagc tgtcgtcacc tgcttcttgg ccgtgcctgt tattaaaaag aattcctacc 2881 tggtcttagc gggcctcgcc gatgggcttg tggctgtgtt tcccgtggtg cggggcaccc 2941 caaaggacag ctgctcctac ctgtgctcac acacagccaa caggtccaag ttcagcatcg 3001 cggatgaaga cgcacggcag aacccctacc cagtgaaggc catggaggtg gtcaacagcg 3061 gctctgaggt ctggtacagc aatgggccgg gcctccttgt catcgactgt gcctccctgg 3121 agatctgcag gcggctggag ccctacatgg ccccctccat ggttacgtca gtcgtgtgca 3181 gctctgaggg cagaggggag gaggtcgtct ggtgcctgga tgacaaggcc aactccttgg 3241 tgatgtacca ctccaccacc taccagctgt gtgcccggta cttctgcggg gtccccagcc 3301 ccctcaggga catgtttccc gtgcggccct tggacacgga acccccggca gccagccaca 3361 cggccaaccc aaaggtgcct gagggggact ccatcgcgga cgtgagcatc atgtacagtg 3421 aggagctggg cacgcagatc ctgatccacc aggaatcact cactgactac tgctccatgt 3481 cctcctactc ctcatcccca ccccgccagg ctgccaggtc cccctcaagc ctccccagct 3541 ccccagcaag ttcttccagt gtgcctttct ccaccgactg cgaggactca gacatgctac 3601 atacgcccgg tgctgcctcc gacaggtctg agcatgacct gacccccatg gacggggaga 3661 ccttcagcca gcacctgcag gccgtgaaga tcctcgccgt cagagacctc atttgggtcc 3721 ccaggcgcgg tGGAGATGTT ATCGTCATTG GCCTGGAGAA GGATTCTGGC GCCCAGCGGG 3781 GCCGAGTCAT TGCCGTCTTA AAAGCCCGAG AGCTGACTCC GCATGGGGTG CTGGTGGATG 3841 CTGCCGTGGT GGCAAAGGAC ACTGTTGTGT GCACCTTTGA AAATGAAAAC ACAGAGTGGT 3901 GCCTGGCCGT CTGGAGGGGC TGGGGCGCCA GGGAGTTCGA CATTTTCTAC CAGTCCTACG 3961 AGGAGCTGGG CCGGCTGGAG GCTTGCACTC GCAAGAGAAG GTAGGACCCA GCTTTCTTGT 4021 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 4081 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 4141 GAAAGGACGA CTGCCTTATA AACTAAGGTC TGGCACGCGT TAAGTCgaca atcaacctct 4201 ggattacaaa atttgtgaaa gatt