Construct: ORF TRCN0000489287

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020202.1_s317c1
DNA Barcode:
TTCGACCCTCTTCGTTCTTGTCGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FGGY (55277)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489287
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55277 FGGY FGGY carbohydrate kinase do... NM_001278224.2 100% 100%
2 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448227.1 73.4% 73.4% 1_273del
3 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448229.1 73.4% 73.4% 1_273del
4 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001677.1 68.6% 68.6% 1_273del;597_668del
5 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001678.1 68.6% 68.6% 1_273del;597_668del
6 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350797.2 60.4% 60.4% 1_495del
7 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350796.2 57.4% 57.4% 1_561del
8 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001673.2 57.4% 57.4% 1_561del
9 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448220.1 57.4% 57.4% 1_561del
10 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001668.1 57.1% 57.1% 1_495del;819_890del
11 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001669.2 57.1% 57.1% 1_495del;819_890del
12 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001670.2 57.1% 57.1% 1_495del;819_890del
13 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001671.1 57.1% 57.1% 1_495del;819_890del
14 human 55277 FGGY FGGY carbohydrate kinase do... NM_001244714.2 54.4% 54.4% 1_633del
15 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001656.1 54.4% 54.4% 1_561del;885_956del
16 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001657.2 54.4% 54.4% 1_561del;885_956del
17 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001659.2 54.4% 54.4% 1_561del;885_956del
18 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001660.2 54.4% 54.4% 1_561del;885_956del
19 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001661.2 54.4% 54.4% 1_561del;885_956del
20 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001662.2 54.4% 54.4% 1_561del;885_956del
21 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001663.2 54.4% 54.4% 1_561del;885_956del
22 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001664.2 54.4% 54.4% 1_561del;885_956del
23 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350795.2 51.7% 51.7% 1_633del;957_1028del
24 human 55277 FGGY FGGY carbohydrate kinase do... XM_011541736.3 51.7% 51.7% 1_633del;957_1028del
25 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001655.1 51.7% 51.7% 1_633del;957_1028del
26 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350792.2 50.9% 50.9% 1_729del
27 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350793.2 50.9% 50.9% 1_729del
28 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448185.1 48.5% 48.5% 1_729del;1053_1124del
29 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448196.1 48.5% 48.5% 1_729del;1053_1124del
30 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350791.2 45.7% 45.7% 1_897del
31 human 55277 FGGY FGGY carbohydrate kinase do... NM_018291.4 45.7% 45.7% 1_897del
32 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001645.1 45.7% 45.7% 1_897del
33 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001646.1 45.7% 45.7% 1_897del
34 human 55277 FGGY FGGY carbohydrate kinase do... NM_001113411.2 43.8% 43.8% 1_897del;1221_1292del
35 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350790.2 43.8% 43.8% 1_897del;1221_1292del
36 human 55277 FGGY FGGY carbohydrate kinase do... XM_011541730.1 43.8% 43.8% 1_897del;1221_1292del
37 human 55277 FGGY FGGY carbohydrate kinase do... XM_011541731.1 43.8% 43.8% 1_897del;1221_1292del
38 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001643.2 43.8% 43.8% 1_897del;1221_1292del
39 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448176.1 43.8% 43.8% 1_897del;1221_1292del
40 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350799.2 43.7% 40.2% 1_561del;1080_1081ins95;1137_1138ins85
41 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350798.2 41.4% 38.1% (many diffs)
42 human 55277 FGGY FGGY carbohydrate kinase do... NM_001350794.2 34.8% 32.1% 1_897del;1416_1417ins95;1473_1474ins85
43 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001652.1 34.8% 32.1% 1_897del;1416_1417ins95;1473_1474ins85
44 human 55277 FGGY FGGY carbohydrate kinase do... XM_024448207.1 34.8% 32.1% 1_897del;1416_1417ins95;1473_1474ins85
45 human 55277 FGGY FGGY carbohydrate kinase do... XM_017001649.1 33.3% 30.7% (many diffs)
46 human 55277 FGGY FGGY carbohydrate kinase do... XR_001737286.1 31.7% 1_1010del;1185_1186ins148;1619_1767del
47 human 55277 FGGY FGGY carbohydrate kinase do... XR_001737285.1 31.3% 1_1033del;1208_1209ins148;1642_1790del
48 human 55277 FGGY FGGY carbohydrate kinase do... XR_001737284.1 30.6% 1_1078del;1253_1254ins148;1687_1835del
49 human 55277 FGGY FGGY carbohydrate kinase do... NR_103473.2 28.9% (many diffs)
50 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_006503476.1 89.3% 92.4% (many diffs)
51 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_017320432.1 65.7% 67.9% (many diffs)
52 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_006503474.1 51.4% 53% (many diffs)
53 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240637.2 42.1% 38.4% (many diffs)
54 mouse 75578 Fggy FGGY carbohydrate kinase do... NM_001113412.1 40.9% 42.2% (many diffs)
55 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_006503461.3 40.9% 42.2% (many diffs)
56 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240633.2 40.3% 41.6% (many diffs)
57 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240636.1 35.5% 35.8% (many diffs)
58 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240635.1 35.2% 34.8% (many diffs)
59 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240639.2 34.4% 34.8% (many diffs)
60 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_017320430.1 31.7% 25% (many diffs)
61 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_017320429.1 31.2% 24.7% (many diffs)
62 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_006503468.3 28.7% 23.5% (many diffs)
63 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_006503472.3 28% 23.9% (many diffs)
64 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240644.1 28% 23.3% (many diffs)
65 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_006503470.3 27.7% 23.6% (many diffs)
66 mouse 75578 Fggy FGGY carbohydrate kinase do... XM_011240643.2 27.6% 23.6% (many diffs)
67 mouse 75578 Fggy FGGY carbohydrate kinase do... XR_001784213.1 19.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
828
ORF length:
756
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggggatc agcaaagacc cgatttttgt accaggcgtc tgggggcctt
121atttctcagc catggtacct gggttctggc tgaatgaagg tggtcagagc gttactggaa
181aattgataga ccacatggta caaggccatg ctgcttttcc agaactacaa gtaaaggcca
241cagccagatg ccagagtata tatgcatatt tgaacagtca cctggatctg attaagaagg
301ctcagcctgt gggtttcctt actgttgatt tacatgtttg gccagatttc catggcaacc
361ggtctccctt agcagatctg acactaaagg gcatggtcac cggattgaaa ctgtctcagg
421accttgatga tcttgccatt ctctacctgg ccacagttca agccattgct ttggggactc
481gcttcattat agaagccatG GAGGCAGCAG GGCACTCAAT CAGTACTCTT TTCCTATGTG
541GAGGCCTCAG CAAGAATCCC CTTTTTGTGC AAATGCATGC GGACATTACT GGCATGCCTG
601TGGTCCTGTC GCAAGAGGTG GAGTCCGTTC TTGTGGGTGC TGCTGTTCTG GGTGCCTGTG
661CCTCAGGGGA TTTCGCTTCT GTACAGGAAG CAATGGCAAA AATGAGCAAA GTTGGGAAAG
721TTGTGTTCCC GAGACTACAG GATAAAAAAT ACTATGATAA GAAATACCAA GTATTCCTGA
781AGCTGGTTGA ACACCAGAAG GAGTATTTGG CGATCATGAA TGATGACTGA GACCCAGCTT
841TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
901CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
961CTTGTGGAAA GGACGATTCG ACCCTCTTCG TTCTTGTCGG ACGCGTTAAG TCgacaatca
1021acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)