Construct: ORF TRCN0000489293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020022.1_s317c1
- DNA Barcode:
- CACACGAACCTGTACAGAGAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSNK2A2 (1459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | NM_001896.4 | 99.9% | 99.7% | 1050_1051insG |
| 2 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_005255800.4 | 85.3% | 85.1% | 213_214ins153;897_898insG |
| 3 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_017022945.2 | 65.1% | 61.9% | (many diffs) |
| 4 | human | 1459 | CSNK2A2 | casein kinase 2 alpha 2 | XM_005255801.3 | 60.7% | 60.6% | 0_1ins411;639_640insG |
| 5 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | NM_009974.3 | 94.7% | 98.5% | (many diffs) |
| 6 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_017312556.1 | 89.9% | 93.7% | (many diffs) |
| 7 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530641.3 | 80.3% | 84% | (many diffs) |
| 8 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530642.1 | 56.8% | 59.5% | (many diffs) |
| 9 | mouse | 13000 | Csnk2a2 | casein kinase 2, alpha prim... | XM_006530643.3 | 56.8% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1122
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcccggcccg gccgcgggca gcagggcccg ggtctacgcc gaggtgaaca 121 gtctgaggag ccgcgagtac tgggactacg aggctcacgt cccgagctgg ggtaatcaag 181 atgattacca actggttcga aaacttggtc ggggaaaata tagtgaagta tttgaggcca 241 ttaatatcac caacaatgag agagtggttg taaaaatcct gaagccagtg aagaaaaaga 301 agataaaacg agaggttaag attctggaga accttcgtgg tggaacaaat atcattaagc 361 tgattgacac tgtaaaggac cccgtgtcaa agacaccagc tttggtattt gaatatatca 421 ataatacaga ttttaagcaa ctctaccaga tcctgacaga ctttgatatc cggttttata 481 tgtatgaact acttaaagct ctggattact gccacagcaa gggaatcatg cacagggatg 541 tgaaacctca caatgtcatg atagatcacc aacagaaaaa gctgcgactg atagattggg 601 gtctggcaga attctatcat cctgctcagg agtacaatgt tcgtgtagcc tcaaggtact 661 tcaagggacc agagctcctc gtggactatc agatgtatga ttatagcttg gacatgtgga 721 gtttgggctg tatgttagca agcatgatct ttcGAAGGGA ACCATTCTTC CATGGACAGG 781 ACAACTATGA CCAGCTTGTT CGCATTGCCA AGGTTCTGGG TACAGAAGAA CTGTATGGGT 841 ATCTGAAGAA GTATCACATA GACCTAGATC CACACTTCAA CGATATCCTG GGACAACATT 901 CACGGAAACG CTGGGAAAAC TTTATCCATA GTGAGAACAG ACACCTTGTC AGCCCTGAGG 961 CCCTAGATCT TCTGGACAAA CTTCTGCGAT ACGACCATCA ACAGAGACTG ACTGCCAAAG 1021 AGGCCATGGA GCACCCATAC TTCTACCCTG TGGTGAAGGA GCAGTCCCAG CCTTGTGCAG 1081 ACAATGCTGT GCTTTCCAGT GGTCTCACGG CAGCACGAGA CCCAGCTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGACACACG AACCTGTACA GAGAAATGAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt