Construct: ORF TRCN0000489303
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021925.1_s317c1
- DNA Barcode:
- ACTAGCTCCGCGCACTTAACCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MET (4233)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489303
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_000245.4 | 99.9% | 100% | 2121T>C;2124A>G |
| 2 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001127500.3 | 98.6% | 98.7% | 2121T>C;2124A>G;2263_2316del |
| 3 | human | 4233 | MET | MET proto-oncogene, recepto... | XM_011516223.1 | 98.6% | 98.6% | 1_57del;2178T>C;2181A>G |
| 4 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001324402.2 | 69% | 69% | 0_1ins1290;831T>C;834A>G |
| 5 | human | 4233 | MET | MET proto-oncogene, recepto... | XM_006715990.2 | 69% | 69% | 0_1ins1290;831T>C;834A>G |
| 6 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001324401.2 | 66.6% | 65.9% | (many diffs) |
| 7 | human | 4233 | MET | MET proto-oncogene, recepto... | XR_001744772.1 | 61% | (many diffs) | |
| 8 | mouse | 17295 | Met | met proto-oncogene | NM_008591.2 | 85.7% | 88.9% | (many diffs) |
| 9 | mouse | 17295 | Met | met proto-oncogene | XM_011241042.2 | 85.7% | 88.9% | (many diffs) |
| 10 | mouse | 17295 | Met | met proto-oncogene | XM_011241043.2 | 85.7% | 88.9% | (many diffs) |
| 11 | mouse | 17295 | Met | met proto-oncogene | XM_006505002.3 | 82.4% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4242
- ORF length:
- 4170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggcc cccgctgtgc ttgcacctgg catcctcgtg ctcctgttta 121 ccttggtgca gaggagcaat ggggagtgta aagaggcact agcaaagtcc gagatgaatg 181 tgaatatgaa gtatcagctt cccaacttca ccgcggaaac acccatccag aatgtcattc 241 tacatgagca tcacattttc cttggtgcca ctaactacat ttatgtttta aatgaggaag 301 accttcagaa ggttgctgag tacaagactg ggcctgtgct ggaacaccca gattgtttcc 361 catgtcagga ctgcagcagc aaagccaatt tatcaggagg tgtttggaaa gataacatca 421 acatggctct agttgtcgac acctactatg atgatcaact cattagctgt ggcagcgtca 481 acagagggac ctgccagcga catgtctttc cccacaatca tactgctgac atacagtcgg 541 aggttcactg catattctcc ccacagatag aagagcccag ccagtgtcct gactgtgtgg 601 tgagcgccct gggagccaaa gtcctttcat ctgtaaagga ccggttcatc aacttctttg 661 taggcaatac cataaattct tcttatttcc cagatcatcc attgcattcg atatcagtga 721 gaaggctaaa ggaaacgaaa gatggtttta tgtttttgac ggaccagtcc tacattgatg 781 ttttacctga gttcagagat tcttacccca ttaagtatgt ccatgccttt gaaagcaaca 841 attttattta cttcttgacg gtccaaaggg aaactctaga tgctcagact tttcacacaa 901 gaataatcag gttctgttcc ataaactctg gattgcattc ctacatggaa atgcctctgg 961 agtgtattct cacagaaaag agaaaaaaga gatccacaaa gaaggaagtg tttaatatac 1021 ttcaggctgc gtatgtcagc aagcctgggg cccagcttgc tagacaaata ggagccagcc 1081 tgaatgatga cattcttttc ggggtgttcg cacaaagcaa gccagattct gccgaaccaa 1141 tggatcgatc tgccatgtgt gcattcccta tcaaatatgt caacgacttc ttcaacaaga 1201 tcgtcaacaa aaacaatgtg agatgtctcc agcattttta cggacccaat catgagcact 1261 gctttaatag gacacttctg agaaattcat caggctgtga agcgcgccgt gatgaatatc 1321 gaacagagtt taccacagct ttgcagcgcg ttgacttatt catgggtcaa ttcagcgaag 1381 tcctcttaac atctatatcc accttcatta aaggagacct caccatagct aatcttggga 1441 catcagaggg tcgcttcatg caggttgtgg tttctcgatc aggaccatca acccctcatg 1501 tgaattttct cctggactcc catccagtgt ctccagaagt gattgtggag catacattaa 1561 accaaaatgg ctacacactg gttatcactg ggaagaagat cacgaagatc ccattgaatg 1621 gcttgggctg cagacatttc cagtcctgca gtcaatgcct ctctgcccca ccctttgttc 1681 agtgtggctg gtgccacgac aaatgtgtgc gatcggagga atgcctgagc gggacatgga 1741 ctcaacagat ctgtctgcct gcaatctaca aggttttccc aaatagtgca ccccttgaag 1801 gagggacaag gctgaccata tgtggctggg actttggatt tcggaggaat aataaatttg 1861 atttaaagaa aactagagtt ctccttggaa atgagagctg caccttgact ttaagtgaga 1921 gcacgatgaa tacattgaaa tgcacagttg gtcctgccat gaataagcat ttcaatatgt 1981 ccataattat ttcaaatggc cacgggacaa cacaatacag tacattctcc tatgtggatc 2041 ctgtaataac aagtatttcg ccgaaatacg gtcctatggc tggtggcact ttacttactt 2101 taactggaaa ttacctaaac agtgggaatt ctagacacat ttcaattggt ggaaaaacat 2161 gtactttaaa aagtgtgtca aacagtattc tcgagtgtta taccccagcc caaaccattt 2221 caactgagtt tgctgttaaa ttgaaaattg acttagccaa ccgagagaca agcatcttca 2281 gttaccgtga agatcccatt gtctatgaaa ttcatccaac caaatctttt attagtggtg 2341 ggagcacaat aacaggtgtt gggaaaaacc tgaattcagt tagtgtcccg agaatggtca 2401 taaatgtgca tgaagcagga aggaacttta cagtggcatg tcaacatcgc tctaattcag 2461 agataatctg ttgtaccact ccttccctgc aacagctgaa tctgcaactc cccctgaaaa 2521 ccaaagcctt tttcatgtta gatgggatcc tttccaaata ctttgatctc atttatgtac 2581 ataatcctgt gtttaagcct tttgaaaagc cagtgatgat ctcaatgggc aatgaaaatg 2641 tactggaaat taagggaaat gatattgacc ctgaagcagt taaaggtgaa gtgttaaaag 2701 ttggaaataa gagctgtgag aatatacact tacattctga agccgtttta tgcacggtcc 2761 ccaatgacct gctgaaattg aacagcgagc taaatataga gtggaagcaa gcaatttctt 2821 caaccgtcct tggaaaagta atagttcaac cagatcagaa tttcacagga ttgattgctg 2881 gtgttgtctc aatatcaaca gcactgttat tactacttgg gtttttcctg tggctgaaaa 2941 agagaaagca aattaaagat ctgggcagtg aattagttcg ctacgatgca agagtacaca 3001 ctcctcattt ggataggctt gtaagtgccc gaagtgtaag cccaactaca gaaatggttt 3061 caaatgaatc tgtagactac cgagctactt ttccagaaga tcagtttcct aattcatctc 3121 agaacggttc atgccgacaa gtgcagtatc ctctgacaga catgtccccc atcctaacta 3181 gtggggactc tgatatatcc agtccattac tgcaaaatac tgtccacatt gacctcagtg 3241 ctctaaatcc agagctggtc caggcagtgc agcatgtagt gattgggccc agtagcctga 3301 ttgtgcattt caatgaagtc ataggaagag ggcattttgg ttgtgtatat catgggactt 3361 tgttggacaa tgatggcaag aaaattcact gtgctgtgaa atccttgaac agaatcactg 3421 acataggaga agtttcccaa tttctgaccg agggaatcat catgaaagat tttagtcatc 3481 ccaatgtcct ctcgctcctg ggaatctgcc tgcgaagtga agggtctccg ctggtggtcc 3541 taccatacat gaaacatgga gatcttcgaa atttcattcg aaatgagact cataatccaa 3601 ctgtaaaaga tcttattggc tttggtcttc aagtagccaa aggcatgaaa tatcttgcaa 3661 gcaaaaagtt tgtccacaga gacttggctg caagaaactg tatgctggat gaaaaattca 3721 cagtcaaggt tgctgatttt ggtcttgcca gagacatgta tgataaagaa tactatagtg 3781 tacacaacaa aacaggtgca aagctgccag tgaagtggat ggctttggaa agtctgcaaa 3841 ctcaaaagtt taccaccaag tcagatgtgt ggtcctttGG CGTGCTCCTC TGGGAGCTGA 3901 TGACAAGAGG AGCCCCACCT TATCCTGACG TAAACACCTT TGATATAACT GTTTACTTGT 3961 TGCAAGGGAG AAGACTCCTA CAACCCGAAT ACTGCCCAGA CCCCTTATAT GAAGTAATGC 4021 TAAAATGCTG GCACCCTAAA GCCGAAATGC GCCCATCCTT TTCTGAACTG GTGTCCCGGA 4081 TATCAGCGAT CTTCTCTACT TTCATTGGGG AGCACTATGT CCATGTGAAC GCTACTTATG 4141 TGAACGTAAA ATGTGTCGCT CCGTATCCTT CTCTGTTGTC ATCAGAAGAT AACGCTGATG 4201 ATGAGGTGGA CACACGACCA GCCTCCTTCT GGGAGACATC ATAGGACCCA GCTTTCTTGT 4261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 4321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 4381 GAAAGGACGA ACTAGCTCCG CGCACTTAAC CCAAACGCGT TAAGTCgaca atcaacctct 4441 ggattacaaa atttgtgaaa gatt