Construct: ORF TRCN0000489311

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021408.1_s317c1
DNA Barcode:
GAGCCAGCTGAAAACATACTTGAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PAK1 (5058)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489311
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5058 PAK1 p21 (RAC1) activated kinase 1 NM_001128620.1 99.8% 99.8% 532_534delGAT
2 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545080.3 99.8% 99.8% 532_534delGAT
3 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545081.2 99.8% 99.8% 532_534delGAT
4 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545082.2 99.8% 99.8% 532_534delGAT
5 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545083.2 99.8% 99.8% 532_534delGAT
6 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_011545084.3 99.8% 99.8% 532_534delGAT
7 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448557.1 99.8% 99.8% 532_534delGAT
8 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448558.1 99.8% 99.8% 532_534delGAT
9 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448559.1 99.8% 99.8% 532_534delGAT
10 human 5058 PAK1 p21 (RAC1) activated kinase 1 NM_002576.4 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
11 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017846.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
12 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017847.2 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
13 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017848.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
14 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017849.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
15 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_017017850.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
16 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448560.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
17 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448561.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
18 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448562.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
19 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448563.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
20 human 5058 PAK1 p21 (RAC1) activated kinase 1 XM_024448564.1 95.4% 94.2% 532_534delGAT;1550_1551ins49;1611_1635del
21 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... NM_011035.2 88.3% 93.6% (many diffs)
22 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_017322028.1 88.3% 93.6% (many diffs)
23 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_006507434.1 87% 92.3% (many diffs)
24 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_006507436.3 87% 92.3% (many diffs)
25 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_006507437.2 87% 92.3% (many diffs)
26 mouse 18479 Pak1 p21 protein (Cdc42/Rac)-act... XM_011241694.1 87% 92.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
96
ORF end:
1752
ORF length:
1656
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcaccgga tccctggtgg tgacaatgtc aaataacggc ctagacattc
121aagacaaacc cccagcccct ccgatgagaa ataccagcac tatgattgga gccggcagca
181aagatgctgg aaccctaaac catggttcta aacctctgcc tccaaaccca gaggagaaga
241aaaagaagga ccgattttac cgatccattt tacctggaga taaaacaaat aaaaagaaag
301agaaagagcg gccagagatt tctctccctt cagattttga acacacaatt catgtcggtt
361ttgatgctgt cacaggggag tttacgggaa tgccagagca gtgggcccgc ttgcttcaga
421catcaaatat cactaagtcg gagcagaaga aaaacccgca ggctgttctg gatgtgttgg
481agttttacaa ctcgaagaag acatccaaca gccagaaata catgagcttt acagataagt
541cagctgagga ttacaattct tctaatgcct tgaatgtgaa ggctgtgtct gagactcctg
601cagtgccacc agtttcagaa gatgaggatg atgatgatga tgctacccca ccaccagtga
661ttgctccacg cccagagcac acaaaatctg tatacacacg gtctgtgatt gaaccacttc
721ctgtcactcc aactcgggac gtggctacat ctcccatttc acctactgaa aataacacca
781ctccaccaga tgctttgacc cggaatactg agaagcagaa gaagaagcct aaaatgtctg
841atgaggagat cttggagaaa ttacgaagca tagtgagtgt gggcgatcct aagaagaaat
901atacacggtt tgagaagatt ggacaaggtg cttcaggcac cgtgtacaca gcaatggatg
961tggccacagg acaggaggtg gccattaagc agatgaatct tcagcagcag cccaagaaag
1021agctgattat taatgagatc ctggtcatga gggaaaacaa gaacccaaac attgtgaatt
1081acttggacag ttacctcgtg ggagatgagc tgtgggttgt tatggaatac ttggctggag
1141gctccttgac agatgtggtg acagaaactt gcatggatga aggccaaatt gcagctgtgt
1201gccgtgagtg tctgcaggct ctggagttct tgcattcgaa ccaggtcatt cacagagaca
1261tcaagagtga caatattctg ttgggaatgg atggctctgt caagctaact gactttggat
1321tctgtgcaca gataacccca gagcagagca aacggagcac catggtagga accccatact
1381ggatggcacc agaggttgtg acacgaaagg cctatgggcc caaggttgac atctggtccc
1441tgggcatcat ggccatcgaa atgattgaag gGGAGCCTCC ATACCTCAAT GAAAACCCTC
1501TGAGAGCCTT GTACCTCATT GCCACCAATG GGACCCCAGA ACTTCAGAAC CCAGAGAAGC
1561TGTCAGCTAT CTTCCGGGAC TTTCTGAACC GCTGTCTCGA GATGGATGTG GAGAAGAGAG
1621GTTCAGCTAA AGAGCTGCTA CAGGTGAGAA AACTGAGGTT TCAAGTGTTT AGTAACTTTT
1681CCATGATAGC TGCATCAATT CCTGAAGATT GCCAAGCCCC TCTCCAGCCT CACTCCACTG
1741ATTGCTGCAG CTAAGGAGGC AACAAAGAAC AATCACAAGG GTGGGCGCGC CGACCCAGCT
1801TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT
1861TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA
1921TCTTGTGGAA AGGACGAGAG CCAGCTGAAA ACATACTTGA CACGCGTTAA GTCgacaatc
1981aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)