Transcript: Human NM_001321299.2

Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
WNK4 (65266)
Length:
4110
CDS:
1009..3732

Additional Resources:

NCBI RefSeq record:
NM_001321299.2
NBCI Gene record:
WNK4 (65266)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147390 GTACGAGGAAAAGTACGATG pXPR_003 AGG 138 5% 4 0.7481 WNK4 WNK4 75657
2 BRDN0001147338 TGAAGCCGATTACCAGCCAG pXPR_003 TGG 505 19% 6 0.4666 WNK4 WNK4 75656
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321299.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000367551 TCAGACGGATTCGGGAGATTA pLKO_005 2201 CDS 100% 13.200 18.480 N WNK4 n/a
2 TRCN0000367512 AGCGTGAGAAGCTGCGTAAAG pLKO_005 1559 CDS 100% 10.800 15.120 N WNK4 n/a
3 TRCN0000367589 TTGGTCTGTGAAGCCGATTAC pLKO_005 1489 CDS 100% 10.800 15.120 N WNK4 n/a
4 TRCN0000194757 CCATGGTATATAACGAGTTCA pLKO.1 2153 CDS 100% 4.950 6.930 N WNK4 n/a
5 TRCN0000007021 CCGATACCTCAAGTTTGACAT pLKO.1 602 5UTR 100% 4.950 6.930 N WNK4 n/a
6 TRCN0000007022 GATGGATTTCTCAGACGGATT pLKO.1 2191 CDS 100% 4.050 5.670 N WNK4 n/a
7 TRCN0000007020 CCGCTTCTATGATTCGTGGAA pLKO.1 791 5UTR 100% 2.640 3.696 N WNK4 n/a
8 TRCN0000219722 GGAGAGTGCAGCTCCATTATA pLKO.1 3872 3UTR 100% 15.000 12.000 N WNK4 n/a
9 TRCN0000367529 ATTGCAGCTGCCATGGTATAT pLKO_005 2143 CDS 100% 13.200 9.240 N WNK4 n/a
10 TRCN0000367524 AGATGGTGACCTTCCGATTTG pLKO_005 2096 CDS 100% 10.800 7.560 N WNK4 n/a
11 TRCN0000356148 GCTCCTTCAAGACGGTGTATC pLKO_005 637 5UTR 100% 10.800 7.560 N WNK4 n/a
12 TRCN0000377269 GGGATCTCAAGTGCGACAATG pLKO_005 991 5UTR 100% 10.800 7.560 N WNK4 n/a
13 TRCN0000007019 CCAGCTACTCATCTACCACTT pLKO.1 1721 CDS 100% 4.050 2.835 N WNK4 n/a
14 TRCN0000199167 CCTGGCATCATGCGAAGGAAC pLKO.1 3628 CDS 100% 1.350 0.945 N WNK4 n/a
15 TRCN0000196673 GCCAAACATATGTGAACTGTT pLKO.1 3900 3UTR 100% 0.000 0.000 N WNK4 n/a
16 TRCN0000007023 GCTGCGTAAAGCAAGGGAATT pLKO.1 1569 CDS 100% 0.000 0.000 N WNK4 n/a
17 TRCN0000356149 TCAAGTTTGACATCGAGATTG pLKO_005 610 5UTR 100% 10.800 6.480 N WNK4 n/a
18 TRCN0000028835 CGATACCTCAAGTTTGACATT pLKO.1 603 5UTR 100% 4.950 3.960 N Wnk4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321299.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489323 AGGCTACCCCTGATCACTCTATTA pLX_317 9.8% 69.2% 63.2% V5 (not translated due to prior stop codon) 0_1ins919;250_251ins89;1149_1286del n/a
2 ccsbBroadEn_15142 pDONR223 54.5% 67.9% 4.7% None (many diffs) n/a
3 ccsbBroad304_15142 pLX_304 0% 67.9% 4.7% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000474897 TCCGATTATTTCCCTTACGATCAA pLX_317 11.3% 67.9% 4.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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