Construct: ORF TRCN0000489327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020250.1_s317c1
- DNA Barcode:
- CGATATTTAACATCTACACACTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NME7 (29922)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29922 | NME7 | NME/NM23 family member 7 | NM_013330.5 | 99.9% | 100% | 207T>C |
| 2 | human | 29922 | NME7 | NME/NM23 family member 7 | NM_197972.2 | 90.3% | 90.4% | 0_1ins108;99T>C |
| 3 | human | 29922 | NME7 | NME/NM23 family member 7 | NR_104229.1 | 65.5% | (many diffs) | |
| 4 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_001293313.1 | 81.1% | 84% | (many diffs) |
| 5 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_138314.4 | 81.1% | 84% | (many diffs) |
| 6 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | XM_006496692.2 | 77.2% | 79.7% | (many diffs) |
| 7 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | XM_006496693.3 | 77.2% | 79.7% | (many diffs) |
| 8 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_178071.6 | 55.9% | 56.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1200
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatcat agtgaaagat tcgttttcat tgcagagtgg tatgatccaa 121 atgcttcact tcttcgacgt tatgagcttt tattttaccc aggggatgga tctgttgaaa 181 tgcatgatgt aaagaatcat cgcacctttt taaagcggac caaatatgat aacctgcact 241 tggaagattt atttataggc aacaaagtga atgtcttctc tcgacaactg gtattaattg 301 actatgggga tcaatataca gctcgccagc tgggcagtag gaaagaaaaa acgctagccc 361 taattaaacc agatgcaata tcaaaggctg gagaaataat tgaaataata aacaaagctg 421 gatttactat aaccaaactc aaaatgatga tgctttcaag gaaagaagca ttggattttc 481 atgtagatca ccagtcaaga ccctttttca atgagctgat ccagtttatt acaactggtc 541 ctattattgc catggagatt ttaagagatg atgctatatg tgaatggaaa agactgctgg 601 gacctgcaaa ctctggagtg gcacgcacag atgcttctga aagcattaga gccctctttg 661 gaacagatgg cataagaaat gcagcgcatg gccctgattc ttttgcttct gcggccagag 721 aaatggagtt gttttttcct tcaagtggag gttgtgggcc ggcaaacact gctaaattta 781 ctaattgtac ctgttgcatt gttaaacccc atgctgtcag tgaaggactg ttgggaaaga 841 tcctgatggc tatccgagat gcaggttttg aaatcTCAGC TATGCAGATG TTCAATATGG 901 ATCGGGTTAA TGTTGAGGAA TTCTATGAAG TTTATAAAGG AGTAGTGACC GAATATCATG 961 ACATGGTGAC AGAAATGTAT TCTGGCCCTT GTGTAGCAAT GGAGATTCAA CAGAATAATG 1021 CTACAAAGAC ATTTCGAGAA TTTTGTGGAC CTGCTGATCC TGAAATTGCC CGGCATTTAC 1081 GCCCTGGAAC TCTCAGAGCA ATCTTTGGTA AAACTAAGAT CCAGAATGCT GTTCACTGTA 1141 CTGATCTGCC AGAGGATGGC CTATTAGAGG TTCAATACTT CTTCAAGATC TTGGATAATT 1201 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGACG ATATTTAACA TCTACACACT GAACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt