Construct: ORF TRCN0000489328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020247.1_s317c1
- DNA Barcode:
- AGTGAATAATCTACATCTGTAAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HSP90B1 (7184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7184 | HSP90B1 | heat shock protein 90 beta ... | NM_003299.3 | 99.8% | 99.8% | 2277C>T;2362_2364delGAA |
| 2 | human | 7190 | HSP90B2P | heat shock protein 90 beta ... | NR_073383.1 | 81% | (many diffs) | |
| 3 | mouse | 22027 | Hsp90b1 | heat shock protein 90, beta... | NM_011631.1 | 89.6% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2478
- ORF length:
- 2406
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagggcc ctgtgggtgc tgggcctctg ctgcgtcctg ctgaccttcg 121 ggtcggtcag agctgacgat gaagttgatg tggatggtac agtagaagag gatctgggta 181 aaagtagaga aggatcaagg acggatgatg aagtagtaca gagagaggaa gaagctattc 241 agttggatgg attaaatgca tcacaaataa gagaacttag agagaagtcg gaaaagtttg 301 ccttccaagc cgaagttaac agaatgatga aacttatcat caattcattg tataaaaata 361 aagagatttt cctgagagaa ctgatttcaa atgcttctga tgctttagat aagataaggc 421 taatatcact gactgatgaa aatgctcttt ctggaaatga ggaactaaca gtcaaaatta 481 agtgtgataa ggagaagaac ctgctgcatg tcacagacac cggtgtagga atgaccagag 541 aagagttggt taaaaacctt ggtaccatag ccaaatctgg gacaagcgag tttttaaaca 601 aaatgactga agcacaggaa gatggccagt caacttctga attgattggc cagtttggtg 661 tcggtttcta ttccgccttc cttgtagcag ataaggttat tgtcacttca aaacacaaca 721 acgataccca gcacatctgg gagtctgact ccaatgaatt ttctgtaatt gctgacccaa 781 gaggaaacac tctaggacgg ggaacgacaa ttacccttgt cttaaaagaa gaagcatctg 841 attaccttga attggataca attaaaaatc tcgtcaaaaa atattcacag ttcataaact 901 ttcctattta tgtatggagc agcaagactg aaactgttga ggagcccatg gaggaagaag 961 aagcagccaa agaagagaaa gaagaatctg atgatgaagc tgcagtagag gaagaagaag 1021 aagaaaagaa accaaagact aaaaaagttg aaaaaactgt ctgggactgg gaacttatga 1081 atgatatcaa accaatatgg cagagaccat caaaagaagt agaagaagat gaatacaaag 1141 ctttctacaa atcattttca aaggaaagtg atgaccccat ggcttatatt cactttactg 1201 ctgaagggga agttaccttc aaatcaattt tatttgtacc cacatctgct ccacgtggtc 1261 tgtttgacga atatggatct aaaaagagcg attacattaa gctctatgtg cgccgtgtat 1321 tcatcacaga cgacttccat gatatgatgc ctaaatacct caattttgtc aagggtgtgg 1381 tggactcaga tgatctcccc ttgaatgttt cccgcgagac tcttcagcaa cataaactgc 1441 ttaaggtgat taggaagaag cttgttcgta aaacgctgga catgatcaag aagattgctg 1501 atgataaata caatgatact ttttggaaag aatttggtac caacatcaag cttggtgtga 1561 ttgaagacca ctcgaatcga acacgtcttg ctaaacttct taggttccag tcttctcatc 1621 atccaactga cattactagc ctagaccagt atgtggaaag aatgaaggaa aaacaagaca 1681 aaatctactt catggctggg tccagcagaa aagaggctga atcttctcca tttgttgagc 1741 gacttctgaa aaagggctat gaagttattt acctcacaga acctgtggat gaatactgta 1801 ttcaggccct tcccgaattt gatgggaaga ggttccagaa tgttgccaag gaaggagtga 1861 agttcgatga aagtgagaaa actaaggaga gtcgtgaagc agttgagaaa gaatttgagc 1921 ctctgctgaa ttggatgaaa gataaagccc ttaaggacaa gattgaaaag gctgtggtgt 1981 ctcagcgcct gacagaatct ccgtgtgctt tggtggccag ccagtacgga tggtctggca 2041 acatggagag aatcatgaaa gcacaagcgt accaaacggg caaggacatc tctacaaatt 2101 actatgcgag tcagaagaaa acatttgaaa ttaaTCCCAG ACACCCGCTG ATCAGAGACA 2161 TGCTTCGACG AATTAAGGAA GATGAAGATG ATAAAACAGT TTTGGATCTT GCTGTGGTTT 2221 TGTTTGAAAC AGCAACGCTT CGGTCAGGGT ATCTTTTACC AGACACTAAA GCATATGGAG 2281 ATAGAATAGA AAGAATGCTT CGCCTCAGTT TGAACATTGA CCCTGATGCA AAGGTGGAAG 2341 AAGAGCCTGA AGAAGAACCT GAAGAGACAG CAGAAGACAC AACAGAAGAC ACAGAGCAAG 2401 ACGAAGATGA AGAAATGGAT GTGGGAACAG ATGAAGAAGA AACAGCAAAG GAATCTACAG 2461 CTGAAAAAGA TGAATTGTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2521 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2581 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGTG AATAATCTAC 2641 ATCTGTAAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt