Construct: ORF TRCN0000489332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020148.1_s317c1
- DNA Barcode:
- CTTAAACCGTTGGGACCTTGAGCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CSNK2A1 (1457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_001362770.2 | 100% | 100% | |
| 2 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_001362771.2 | 100% | 100% | |
| 3 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_001895.4 | 100% | 100% | |
| 4 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_177559.3 | 100% | 100% | |
| 5 | human | 283106 | CSNK2A3 | casein kinase 2 alpha 3 | NM_001256686.2 | 99.3% | 98.4% | (many diffs) |
| 6 | human | 1457 | CSNK2A1 | casein kinase 2 alpha 1 | NM_177560.3 | 65.2% | 65.2% | 0_1ins408 |
| 7 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | NM_007788.3 | 94.5% | 98.7% | (many diffs) |
| 8 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_006498657.4 | 94.5% | 98.7% | (many diffs) |
| 9 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_011239272.3 | 94.5% | 98.7% | (many diffs) |
| 10 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_030247074.1 | 94.5% | 98.7% | (many diffs) |
| 11 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_030247075.1 | 94.5% | 98.7% | (many diffs) |
| 12 | mouse | 12995 | Csnk2a1 | casein kinase 2, alpha 1 po... | XM_030247076.1 | 94.5% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1245
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcggga cccgtgccaa gcagggccag agtttacaca gatgttaata 121 cacacagacc tcgagaatac tgggattacg agtcacatgt ggtggaatgg ggaaatcaag 181 atgactacca gctggttcga aaattaggcc gaggtaaata cagtgaagta tttgaagcca 241 tcaacatcac aaataatgaa aaagttgttg ttaaaattct caagccagta aaaaagaaga 301 aaattaagcg tgaaataaag attttggaga atttgagagg aggtcccaac atcatcacac 361 tggcagacat tgtaaaagac cctgtgtcac gaacccccgc cttggttttt gaacacgtaa 421 acaacacaga cttcaagcaa ttgtaccaga cgttaacaga ctatgatatt cgattttaca 481 tgtatgagat tctgaaggcc ctggattatt gtcacagcat gggaattatg cacagagatg 541 tcaagcccca taatgtcatg attgatcatg agcacagaaa gctacgacta atagactggg 601 gtttggctga gttttatcat cctggccaag aatataatgt ccgagttgct tcccgatact 661 tcaaaggtcc tgagctactt gtagactatc agatgtacga ttatagtttg gatatgtgga 721 gtttgggttg tatgctggca agtatgatct ttcggaagga gccatttttc catggacatg 781 acaattatga tcagttggtg aggatagcca aggttctggg gacagaagat ttatatgact 841 atattgacaa atacaacatt gaattagatc cacgtttcaa tgatatcttg GGCAGACACT 901 CTCGAAAGCG ATGGGAACGC TTTGTCCACA GTGAAAATCA GCACCTTGTC AGCCCTGAGG 961 CCTTGGATTT CCTGGACAAA CTGCTGCGAT ATGACCACCA GTCACGGCTT ACTGCAAGAG 1021 AGGCAATGGA GCACCCCTAT TTCTACACTG TTGTGAAGGA CCAGGCTCGA ATGGGTTCAT 1081 CTAGCATGCC AGGGGGCAGT ACGCCCGTCA GCAGCGCCAA TATGATGTCA GGGATTTCTT 1141 CAGTGCCAAC CCCTTCACCC CTTGGACCTC TGGCAGGCTC ACCAGTGATT GCTGCTGCCA 1201 ACCCCCTTGG GATGCCTGTT CCAGCTGCCG CTGGCGCTCA GCAGTGAGAC CCAGCTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGACTTAAAC CGTTGGGACC TTGAGCTACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt