Construct: ORF TRCN0000489348

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021929.1_s317c1
DNA Barcode:
TTCGATTCCAATTCTGAGTTCTTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAPT (4137)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489348
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4137 MAPT microtubule associated prot... NM_016834.5 99.9% 99.7% 728C>T
2 human 4137 MAPT microtubule associated prot... NM_001123067.3 92.8% 92.7% 131_217del;815C>T
3 human 4137 MAPT microtubule associated prot... NM_016841.5 91.9% 91.9% 648_649ins93
4 human 4137 MAPT microtubule associated prot... NM_005910.5 86.7% 86.6% 132_305del;902C>T
5 human 4137 MAPT microtubule associated prot... NM_001203251.2 85.4% 85.4% 131_217del;735_736ins93
6 human 4137 MAPT microtubule associated prot... XM_005257371.4 85.2% 85% 252_449del;926C>T
7 human 4137 MAPT microtubule associated prot... XM_005257370.4 80% 79.9% 131_217del;339_536del;1013C>T
8 human 4137 MAPT microtubule associated prot... NM_001203252.1 79.8% 79.8% 132_305del;822_823ins93
9 human 4137 MAPT microtubule associated prot... XM_005257369.4 75.4% 75.3% 132_305del;426_623del;1100C>T
10 human 4137 MAPT microtubule associated prot... NM_016835.4 50.4% 50.3% (many diffs)
11 human 4137 MAPT microtubule associated prot... NM_001123066.3 49.3% 49.2% (many diffs)
12 human 4137 MAPT microtubule associated prot... XM_005257367.4 48% 47.9% 132_305del;371_1435del;1967C>T
13 human 4137 MAPT microtubule associated prot... XM_005257366.3 47.5% 47.5% 197_1261del;1317_1514del;1991C>T
14 human 4137 MAPT microtubule associated prot... XM_005257364.4 45.9% 45.8% (many diffs)
15 human 4137 MAPT microtubule associated prot... XM_005257362.4 44.3% 44.3% (many diffs)
16 human 4137 MAPT microtubule associated prot... XM_005257368.4 44.2% 44.2% 132_305del;371_1435del;1887_1888ins93
17 human 4137 MAPT microtubule associated prot... XM_005257365.4 40.8% 40.8% (many diffs)
18 mouse 17762 Mapt microtubule-associated prot... NM_010838.4 83.4% 88.8% (many diffs)
19 mouse 17762 Mapt microtubule-associated prot... NM_001285455.1 78.8% 83.8% (many diffs)
20 mouse 17762 Mapt microtubule-associated prot... XM_006532407.3 77.6% 82.6% (many diffs)
21 mouse 17762 Mapt microtubule-associated prot... NM_001285456.1 75.7% 81% (many diffs)
22 mouse 17762 Mapt microtubule-associated prot... NM_001038609.2 72.7% 77.2% (many diffs)
23 mouse 17762 Mapt microtubule-associated prot... NM_001285454.1 71.5% 76.2% (many diffs)
24 mouse 17762 Mapt microtubule-associated prot... XM_006532409.2 70.4% 75.3% (many diffs)
25 mouse 17762 Mapt microtubule-associated prot... XM_006532408.3 66% 70.4% (many diffs)
26 mouse 17762 Mapt microtubule-associated prot... XM_006532410.3 41.9% 44% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1218
ORF length:
1149
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggctgagccc cgccaggagt tcgaagtgat ggaagatcac gctgggacgt
121acgggttggg ggacaggaaa gatcaggggg gctacaccat gcaccaagac caagagggtg
181acacggacgc tggcctgaaa gctgaagaag caggcattgg agacaccccc agcctggaag
241acgaagctgc tggtcacgtg acccaagctc gcatggtcag taaaagcaaa gacgggactg
301gaagcgatga caaaaaagcc aagggggctg atggtaaaac gaagatcgcc acaccgcggg
361gagcagcccc tccaggccag aagggccagg ccaacgccac caggattcca gcaaaaaccc
421cgcccgctcc aaagacacca cccagctctg gtgaacctcc aaaatcaggg gatcgcagcg
481gctacagcag ccccggctcc ccaggcactc ccggcagccg ctcccgcacc ccgtcccttc
541caaccccacc cacccgggag cccaagaagg tggcagtggt ccgtactcca cccaagtcgc
601cgtcttccgc caagagccgc ctgcagacag cccccgtgcc catgccagac ctgaagaatg
661tcaagtccaa gatcggctcc actgagaacc tgaagcacca gccgggaggc gggaaggtgc
721agataattaa taagaagctg gatcttagca acgtccagtc caagtgtggc tcaaaggata
781atatcaaaca cgtcctggga ggcggcagtg tgcaaatagt ctacaaacca gttgacctga
841gcaaggtgac ctccaagtgt ggctcattag gcaacaTCCA TCATAAACCA GGAGGTGGCC
901AGGTGGAAGT AAAATCTGAG AAGCTTGACT TCAAGGACAG AGTCCAGTCG AAGATTGGGT
961CCCTGGACAA TATCACCCAC GTCCCTGGCG GAGGAAATAA AAAGATTGAA ACCCACAAGC
1021TGACCTTCCG CGAGAACGCC AAAGCCAAGA CAGACCACGG GGCGGAGATC GTGTACAAGT
1081CGCCAGTGGT GTCTGGGGAC ACGTCTCCAC GGCATCTCAG CAATGTCTCC TCCACCGGCA
1141GCATCGACAT GGTAGACTCG CCCCAGCTCG CCACGCTAGC TGACGAGGTG TCTGCCTCCC
1201TGGCCAAGCA GGGTTTGTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1261CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1321AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTCG ATTCCAATTC
1381TGAGTTCTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)