Transcript: Human NM_016834.5

Homo sapiens microtubule associated protein tau (MAPT), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
MAPT (4137)
Length:
5465
CDS:
151..1302

Additional Resources:

NCBI RefSeq record:
NM_016834.5
NBCI Gene record:
MAPT (4137)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016834.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437947 GGCGGGAAGGTGCAGATAATT pLKO_005 790 CDS 100% 15.000 21.000 N MAPT n/a
2 TRCN0000415611 ACAGAGTCCAGTCGAAGATTG pLKO_005 1019 CDS 100% 10.800 15.120 N MAPT n/a
3 TRCN0000091300 GACAGAGTCCAGTCGAAGATT pLKO.1 1018 CDS 100% 5.625 7.875 N Mapt n/a
4 TRCN0000083977 CAGTGTGCAAATAGTCTACAA pLKO.1 888 CDS 100% 4.950 6.930 N MAPT n/a
5 TRCN0000083975 GTGTGGCTCATTAGGCAACAT pLKO.1 939 CDS 100% 4.950 6.930 N MAPT n/a
6 TRCN0000439119 GTCCCTGGCGGAGGAAATAAA pLKO_005 1063 CDS 100% 15.000 10.500 N MAPT n/a
7 TRCN0000083973 GCAGCAACAAAGGATTTGAAA pLKO.1 1733 3UTR 100% 5.625 3.938 N MAPT n/a
8 TRCN0000083974 CCAGTCCAAGTGTGGCTCAAA pLKO.1 837 CDS 100% 4.950 3.465 N MAPT n/a
9 TRCN0000083976 GCAGTGTGCAAATAGTCTACA pLKO.1 887 CDS 100% 4.950 3.465 N MAPT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016834.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00973 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00973 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480553 GCCGGAGTCGAAAGACCATACAGA pLX_317 33.3% 100% 100% V5 n/a
4 TRCN0000489348 TTCGATTCCAATTCTGAGTTCTTA pLX_317 30.2% 99.9% 99.7% V5 (not translated due to prior stop codon) 728C>T n/a
5 TRCN0000489050 CTCCCAGCGGGGCTTAGTTTTTAA pLX_317 26.8% 99.9% 99.7% V5 (not translated due to prior stop codon) 727C>T n/a
6 TRCN0000489602 TGCAGCAGCAGTCACCCCGGTGCG pLX_317 31.1% 92.9% 92.9% V5 (not translated due to prior stop codon) 130_131ins87 n/a
7 TRCN0000488420 ACCAAGTGGCGACCCGGCACATGC pLX_317 31% 92.8% 92.7% V5 130_131ins87;1149_1150insG n/a
8 TRCN0000489600 CGCCCGGTTAACGGTCCACACAAC pLX_317 30.5% 92.8% 92.7% V5 (not translated due to prior stop codon) 130_131ins87;728C>T n/a
9 TRCN0000489514 ATGGGGGTCTATGAAACTGGAGAA pLX_317 27.4% 92.8% 92.7% V5 (not translated due to prior stop codon) 130_131ins87;1042C>T n/a
10 TRCN0000488627 ATCCATCTCCACAGGTGGATTGCA pLX_317 30.1% 92.8% 92.7% V5 (not translated due to prior stop codon) 130_131ins87;727C>T n/a
11 TRCN0000491761 TCTTCCCTGGCGGTGGTGTGACCC pLX_317 31% 92.8% 92.4% V5 130_131ins87;728C>T;1149_1150insG n/a
12 TRCN0000491690 TCGTACACCCAAAATGCCAAGGTC pLX_317 31% 92.8% 92.4% V5 130_131ins87;1042C>T;1149_1150insG n/a
13 TRCN0000488826 GGCCCCATGATCTCATTCTTTTAC pLX_317 31.2% 92.7% 92.7% V5 (not translated due to prior stop codon) 130_131ins87;510G>T;740G>A n/a
14 TRCN0000491287 TTTAATTGATCTAATCTACTCAAT pLX_317 24.7% 92.7% 92.4% V5 (many diffs) n/a
15 TRCN0000489210 AGACGGATTCCTACTAATTGTCAG pLX_317 29.1% 91.9% 91.9% V5 (not translated due to prior stop codon) 649_741del n/a
16 TRCN0000489265 TAAAGTCAATTACTGTATAGATGC pLX_317 26.3% 86.8% 86.8% V5 (not translated due to prior stop codon) 131_132ins174 n/a
17 TRCN0000489485 TCAGAACGAACATCTAAGATCTCT pLX_317 28% 86.7% 86.6% V5 (not translated due to prior stop codon) 131_132ins174;727C>T n/a
18 TRCN0000488697 AGATCTAAGGGCGCCCCACTAGCC pLX_317 28.1% 86.7% 86.6% V5 (not translated due to prior stop codon) 131_132ins174;728C>T n/a
19 TRCN0000489653 TCATCCCTTTTTCTTCACCTTATA pLX_317 33.4% 85.3% 85.2% V5 130_131ins87;649_741del;1149_1150insG n/a
20 TRCN0000488263 CTATACCCTTTCCCTAGATCAACC pLX_317 25.7% 82% 82% V5 (not translated due to prior stop codon) 131_132ins174;1087_1149del n/a
21 TRCN0000488920 CAATCAGCTAGGCTTACGTGGGGC pLX_317 30.2% 79.8% 79.8% V5 (not translated due to prior stop codon) 131_132ins174;649_741del n/a
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