Construct: ORF TRCN0000489373
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019889.1_s317c1
- DNA Barcode:
- TTTGAGGCAAATACCATAACCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VEGFA (7422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489373
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171626.1 | 99.6% | 99.4% | 168G>A;573_574insG |
| 2 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171629.1 | 96.5% | 97.3% | (many diffs) |
| 3 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171625.1 | 91% | 90.4% | 168G>A;423_476del;627_628insG |
| 4 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171627.1 | 90.4% | 90.1% | 168G>A;520_521delATinsGC;522_523ins52 |
| 5 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001317010.1 | 89.5% | 99.4% | 168G>A;574_639delinsG |
| 6 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171624.1 | 88.5% | 87.9% | 168G>A;423_494del;645_646insG |
| 7 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001287044.2 | 85% | 84.8% | 0_1ins84;84G>A;489_490insG |
| 8 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001204384.1 | 82.7% | 76.7% | (many diffs) |
| 9 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171623.1 | 82% | 81.5% | 168G>A;422_544del;696_697insG |
| 10 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171628.1 | 76.6% | 76% | 168G>A;422_423ins132;441_442insG |
| 11 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171630.1 | 70.4% | 69.2% | (many diffs) |
| 12 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001025368.3 | 51.3% | 51.3% | 1_540del;708G>A;1113_1114insG |
| 13 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001033756.3 | 50% | 50.2% | (many diffs) |
| 14 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001025367.3 | 48.9% | 48.7% | (many diffs) |
| 15 | human | 7422 | VEGFA | vascular endothelial growth... | NM_003376.6 | 48.2% | 47.9% | (many diffs) |
| 16 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001025369.3 | 46.5% | 46.5% | (many diffs) |
| 17 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001025366.3 | 46.2% | 46% | (many diffs) |
| 18 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001204385.2 | 43% | 40.2% | (many diffs) |
| 19 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001025370.3 | 39.4% | 39.2% | (many diffs) |
| 20 | human | 7422 | VEGFA | vascular endothelial growth... | NM_001171622.2 | 36.3% | 35.7% | (many diffs) |
| 21 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001287057.1 | 89.6% | 89% | (many diffs) |
| 22 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001287056.1 | 80.2% | 78.7% | (many diffs) |
| 23 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001317041.1 | 80.1% | 89% | (many diffs) |
| 24 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001287058.1 | 67.4% | 66.6% | (many diffs) |
| 25 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001110267.1 | 51.1% | 43% | (many diffs) |
| 26 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_009505.4 | 46.5% | 46.2% | (many diffs) |
| 27 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001110266.1 | 46.1% | 37.7% | (many diffs) |
| 28 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001025250.3 | 44% | 43.1% | (many diffs) |
| 29 | mouse | 22339 | Vegfa | vascular endothelial growth... | NM_001025257.3 | 35% | 34.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 648
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacttt ctgctgtctt gggtgcattg gagccttgcc ttgctgctct 121 acctccacca tgccaagtgg tcccaggctg cacccatggc agaaggagga gggcagaatc 181 atcacgaagt ggtgaagttc atggatgtct atcagcgcag ctactgccat ccaatcgaaa 241 ccctggtgga catcttccag gagtaccctg atgagatcga gtacatcttc aagccatcct 301 gtgtgccccT GATGCGATGC GGGGGCTGCT GCAATGACGA GGGCCTGGAG TGTGTGCCCA 361 CTGAGGAGTC CAACATCACC ATGCAGATTA TGCGGATCAA ACCTCACCAA GGCCAGCACA 421 TAGGAGAGAT GAGCTTCCTA CAGCACAACA AATGTGAATG CAGACCAAAG AAAGATAGAG 481 CAAGACAAGA AAATCCCTGT GGGCCTTGCT CAGAGCGGAG AAAGCATTTG TTTGTACAAG 541 ATCCGCAGAC GTGTAAATGT TCCTGCAAAA ACACAGACTC GCGTTGCAAG GCGAGGCAGC 601 TTGAGTTAAA CGAACGTACT TGCAGATGTG ACAAGCCGAG GCGGGACCCA GCTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA TTTGAGGCAA ATACCATAAC CAATACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt