Construct: ORF TRCN0000489403

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020158.1_s317c1
DNA Barcode:
GCAGAGAGGTCTATAAGGTCAGGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CSNK1E (1454)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489403
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 102800317 TPTEP2-CSNK1E TPTEP2-CSNK1E readthrough NM_001289912.2 100% 100%
2 human 1454 CSNK1E casein kinase 1 epsilon NM_001894.4 100% 100%
3 human 1454 CSNK1E casein kinase 1 epsilon NM_152221.3 100% 100%
4 human 1453 CSNK1D casein kinase 1 delta NM_139062.4 77.9% 84.3% (many diffs)
5 human 1453 CSNK1D casein kinase 1 delta NM_001893.6 76.9% 81% (many diffs)
6 mouse 27373 Csnk1e casein kinase 1, epsilon NM_001289898.1 90.7% 98.7% (many diffs)
7 mouse 27373 Csnk1e casein kinase 1, epsilon NM_013767.6 90.7% 98.7% (many diffs)
8 mouse 27373 Csnk1e casein kinase 1, epsilon XM_017316657.1 90.7% 98.7% (many diffs)
9 mouse 27373 Csnk1e casein kinase 1, epsilon XM_017316658.1 90.7% 98.7% (many diffs)
10 mouse 27373 Csnk1e casein kinase 1, epsilon XM_017316659.1 90.7% 98.7% (many diffs)
11 mouse 27373 Csnk1e casein kinase 1, epsilon XM_006521043.3 85.4% 67.9% (many diffs)
12 mouse 27373 Csnk1e casein kinase 1, epsilon XM_011245649.1 85.4% 67.9% (many diffs)
13 mouse 27373 Csnk1e casein kinase 1, epsilon XM_011245650.2 85.4% 67.9% (many diffs)
14 mouse 27373 Csnk1e casein kinase 1, epsilon XM_011245651.1 85.4% 67.9% (many diffs)
15 mouse 27373 Csnk1e casein kinase 1, epsilon XM_011245652.2 85.4% 67.9% (many diffs)
16 mouse 27373 Csnk1e casein kinase 1, epsilon XM_011245653.2 85.4% 67.9% (many diffs)
17 mouse 27373 Csnk1e casein kinase 1, epsilon XM_011245654.2 85.4% 67.9% (many diffs)
18 mouse 27373 Csnk1e casein kinase 1, epsilon NM_001289899.1 75.4% 73.3% (many diffs)
19 mouse 27373 Csnk1e casein kinase 1, epsilon XM_017316660.1 75.4% 73.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1320
ORF length:
1248
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagcta cgtgtgggga acaagtaccg cctgggacgg aagatcggga
121gcgggtcctt cggagatatc tacctgggtg ccaacatcgc ctctggtgag gaagtcgcca
181tcaagctgga gtgtgtgaag acaaagcacc cccagctgca catcgagagc aagttctaca
241agatgatgca gggtggcgtg gggatcccgt ccatcaagtg gtgcggagct gagggcgact
301acaacgtgat ggtcatggag ctgctggggc ctagcctcga ggacctgttc aacttctgtt
361cccgcaaatt cagcctcaag acggtgctgc tcttggccga ccagatgatc agccgcatcg
421agtatatcca ctccaagaac ttcatccacc gggacgtcaa gcccgacaac ttcctcatgg
481ggctggggaa gaagggcaac ctggtctaca tcatcgactt cggcctggcc aagaagtacc
541gggacgcccg cacccaccag cacattccct accgggaaaa caagaacctg accggcacgg
601cccgctacgc ttccatcaac acgcacctgg gcattgagca aagccgtcga gatgacctgg
661agagcctggg ctacgtgctc atgtacttca acctgggctc cctgccctgg caggggctca
721aagcagccac caagcgccag aagtatgaac ggatcagcga gaagaagatg tcaacgccca
781tcgaggtcct ctgcaaaggc tatccctccg aattctcaac atacctcaac ttctgccgct
841ccctgcggtt tgacgacaag cccgactact cttacctacg tcagctcttc cgcaacctct
901tccaccggca gggcttctcc tatGACTACG TCTTTGACTG GAACATGCTG AAATTCGGTG
961CAGCCCGGAA TCCCGAGGAT GTGGACCGGG AGCGGCGAGA ACACGAACGC GAGGAGAGGA
1021TGGGGCAGCT ACGGGGGTCC GCGACCCGAG CCCTGCCCCC TGGCCCACCC ACGGGGGCCA
1081CTGCCAACCG GCTCCGCAGT GCCGCCGAGC CCGTGGCTTC CACGCCAGCC TCCCGCATCC
1141AGCCGGCTGG CAATACTTCT CCCAGAGCGA TCTCGCGGGT CGACCGGGAG AGGAAGGTGA
1201GTATGAGGCT GCACAGGGGT GCGCCCGCCA ACGTCTCCTC CTCAGACCTC ACTGGGCGGC
1261AAGAGGTCTC CCGGATCCCA GCCTCACAGA CAAGTGTGCC ATTTGACCAT CTCGGGAAGT
1321GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1381TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1441GGCTTTATAT ATCTTGTGGA AAGGACGAGC AGAGAGGTCT ATAAGGTCAG GTACGCGTTA
1501AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)