Construct: ORF TRCN0000489403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020158.1_s317c1
- DNA Barcode:
- GCAGAGAGGTCTATAAGGTCAGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CSNK1E (1454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102800317 | TPTEP2-CSNK1E | TPTEP2-CSNK1E readthrough | NM_001289912.2 | 100% | 100% | |
| 2 | human | 1454 | CSNK1E | casein kinase 1 epsilon | NM_001894.4 | 100% | 100% | |
| 3 | human | 1454 | CSNK1E | casein kinase 1 epsilon | NM_152221.3 | 100% | 100% | |
| 4 | human | 1453 | CSNK1D | casein kinase 1 delta | NM_139062.4 | 77.9% | 84.3% | (many diffs) |
| 5 | human | 1453 | CSNK1D | casein kinase 1 delta | NM_001893.6 | 76.9% | 81% | (many diffs) |
| 6 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | NM_001289898.1 | 90.7% | 98.7% | (many diffs) |
| 7 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | NM_013767.6 | 90.7% | 98.7% | (many diffs) |
| 8 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316657.1 | 90.7% | 98.7% | (many diffs) |
| 9 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316658.1 | 90.7% | 98.7% | (many diffs) |
| 10 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316659.1 | 90.7% | 98.7% | (many diffs) |
| 11 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_006521043.3 | 85.4% | 67.9% | (many diffs) |
| 12 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245649.1 | 85.4% | 67.9% | (many diffs) |
| 13 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245650.2 | 85.4% | 67.9% | (many diffs) |
| 14 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245651.1 | 85.4% | 67.9% | (many diffs) |
| 15 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245652.2 | 85.4% | 67.9% | (many diffs) |
| 16 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245653.2 | 85.4% | 67.9% | (many diffs) |
| 17 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245654.2 | 85.4% | 67.9% | (many diffs) |
| 18 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | NM_001289899.1 | 75.4% | 73.3% | (many diffs) |
| 19 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316660.1 | 75.4% | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1320
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcta cgtgtgggga acaagtaccg cctgggacgg aagatcggga 121 gcgggtcctt cggagatatc tacctgggtg ccaacatcgc ctctggtgag gaagtcgcca 181 tcaagctgga gtgtgtgaag acaaagcacc cccagctgca catcgagagc aagttctaca 241 agatgatgca gggtggcgtg gggatcccgt ccatcaagtg gtgcggagct gagggcgact 301 acaacgtgat ggtcatggag ctgctggggc ctagcctcga ggacctgttc aacttctgtt 361 cccgcaaatt cagcctcaag acggtgctgc tcttggccga ccagatgatc agccgcatcg 421 agtatatcca ctccaagaac ttcatccacc gggacgtcaa gcccgacaac ttcctcatgg 481 ggctggggaa gaagggcaac ctggtctaca tcatcgactt cggcctggcc aagaagtacc 541 gggacgcccg cacccaccag cacattccct accgggaaaa caagaacctg accggcacgg 601 cccgctacgc ttccatcaac acgcacctgg gcattgagca aagccgtcga gatgacctgg 661 agagcctggg ctacgtgctc atgtacttca acctgggctc cctgccctgg caggggctca 721 aagcagccac caagcgccag aagtatgaac ggatcagcga gaagaagatg tcaacgccca 781 tcgaggtcct ctgcaaaggc tatccctccg aattctcaac atacctcaac ttctgccgct 841 ccctgcggtt tgacgacaag cccgactact cttacctacg tcagctcttc cgcaacctct 901 tccaccggca gggcttctcc tatGACTACG TCTTTGACTG GAACATGCTG AAATTCGGTG 961 CAGCCCGGAA TCCCGAGGAT GTGGACCGGG AGCGGCGAGA ACACGAACGC GAGGAGAGGA 1021 TGGGGCAGCT ACGGGGGTCC GCGACCCGAG CCCTGCCCCC TGGCCCACCC ACGGGGGCCA 1081 CTGCCAACCG GCTCCGCAGT GCCGCCGAGC CCGTGGCTTC CACGCCAGCC TCCCGCATCC 1141 AGCCGGCTGG CAATACTTCT CCCAGAGCGA TCTCGCGGGT CGACCGGGAG AGGAAGGTGA 1201 GTATGAGGCT GCACAGGGGT GCGCCCGCCA ACGTCTCCTC CTCAGACCTC ACTGGGCGGC 1261 AAGAGGTCTC CCGGATCCCA GCCTCACAGA CAAGTGTGCC ATTTGACCAT CTCGGGAAGT 1321 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGAGC AGAGAGGTCT ATAAGGTCAG GTACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt