Construct: ORF TRCN0000489410

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019715.1_s317c1
DNA Barcode:
TGGGACTGCATACCAAAGATACGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRPK2 (6733)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489410
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6733 SRPK2 SRSF protein kinase 2 NM_001278273.1 99.9% 99.7% 1117A>G;2064_2065insG
2 human 6733 SRPK2 SRSF protein kinase 2 NM_001350738.1 99.9% 99.7% 1117A>G;2064_2065insG
3 human 6733 SRPK2 SRSF protein kinase 2 NM_182691.2 99.9% 99.7% 1117A>G;2064_2065insG
4 human 6733 SRPK2 SRSF protein kinase 2 NM_001350739.1 97.9% 98.2% (many diffs)
5 human 6733 SRPK2 SRSF protein kinase 2 NM_182692.3 97.4% 96.4% (many diffs)
6 human 6733 SRPK2 SRSF protein kinase 2 NM_001350743.1 95.5% 95.4% 1117A>G;1881_1973del;2157_2158insG
7 human 6733 SRPK2 SRSF protein kinase 2 NM_001350744.1 95.5% 95.4% 1117A>G;1881_1973del;2157_2158insG
8 human 6733 SRPK2 SRSF protein kinase 2 XM_006716098.3 95.5% 95.4% 1117A>G;1881_1973del;2157_2158insG
9 human 6733 SRPK2 SRSF protein kinase 2 XM_011516538.3 95.5% 95.4% 1117A>G;1881_1973del;2157_2158insG
10 human 6733 SRPK2 SRSF protein kinase 2 NM_001350742.1 94.8% 94.6% 1_111del;1228A>G;2175_2176insG
11 human 6733 SRPK2 SRSF protein kinase 2 NM_001350741.1 93.3% 92.3% (many diffs)
12 human 6733 SRPK2 SRSF protein kinase 2 NM_001350745.1 93.2% 92.8% 616_617ins138;979A>G;1926_1927insG
13 human 6733 SRPK2 SRSF protein kinase 2 XM_011516536.3 92.9% 92.4% (many diffs)
14 human 6733 SRPK2 SRSF protein kinase 2 XM_017012561.2 92.9% 93.2% (many diffs)
15 human 6733 SRPK2 SRSF protein kinase 2 NM_001350740.1 92.4% 91.5% (many diffs)
16 human 6733 SRPK2 SRSF protein kinase 2 XM_024446893.1 90.9% 90.7% (many diffs)
17 human 6733 SRPK2 SRSF protein kinase 2 XM_024446895.1 90.5% 90.2% (many diffs)
18 human 6733 SRPK2 SRSF protein kinase 2 XM_024446894.1 88.6% 87.8% (many diffs)
19 human 6733 SRPK2 SRSF protein kinase 2 NM_001350746.1 80% 78% (many diffs)
20 human 6733 SRPK2 SRSF protein kinase 2 XR_001744860.2 60.5% (many diffs)
21 human 6733 SRPK2 SRSF protein kinase 2 XR_001744863.2 49.6% 1_173del;1290A>G;2238_4151delinsG
22 human 6733 SRPK2 SRSF protein kinase 2 XR_001744861.2 48.5% (many diffs)
23 human 6733 SRPK2 SRSF protein kinase 2 XR_001744862.2 45.6% (many diffs)
24 human 6733 SRPK2 SRSF protein kinase 2 XR_001744859.2 43.7% (many diffs)
25 mouse 20817 Srpk2 serine/arginine-rich protei... NM_009274.2 89.8% 94% (many diffs)
26 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320769.1 89.8% 94% (many diffs)
27 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535661.3 87.3% 90.8% (many diffs)
28 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249755.2 85.9% 90% (many diffs)
29 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249756.2 85.9% 90% (many diffs)
30 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249757.2 85.9% 90% (many diffs)
31 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535660.3 85.2% 89.2% (many diffs)
32 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320767.1 84.1% 88% (many diffs)
33 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249753.2 83.6% 87% (many diffs)
34 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249754.2 83.5% 87.8% (many diffs)
35 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320768.1 82.4% 86.6% (many diffs)
36 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249751.2 81.7% 85.6% (many diffs)
37 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249752.2 80.6% 84.4% (many diffs)
38 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320771.1 76.5% 80% (many diffs)
39 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249758.2 73.2% 76.5% (many diffs)
40 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320770.1 73.2% 76.5% (many diffs)
41 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320774.1 69.3% 70.2% (many diffs)
42 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320776.1 68.1% 69% (many diffs)
43 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320775.1 68% 70.8% (many diffs)
44 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320777.1 66.7% 69.5% (many diffs)
45 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320773.1 66.3% 67.3% (many diffs)
46 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320772.1 65.1% 66% (many diffs)
47 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249759.2 65% 67.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
2142
ORF length:
2067
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgtca gttaactctg agaagtcgtc ctcttcagaa aggccggagc
121ctcaacagaa agctccttta gttcctcctc ctccaccgcc accaccacca ccaccgccac
181ctttgccaga ccccacaccc ccggagccag aggaggagat cctgggatca gatgatgagg
241agcaagagga ccctgcggac tactgcaaag gtggatatca tccagtgaaa attggagacc
301tcttcaatgg ccggtatcat gttattagaa agcttggatg ggggcacttc tctactgtct
361ggctgtgctg ggatatgcag gggaaaagat ttgttgcaat gaaagttgta aaaagtgccc
421agcattatac ggagacagcc ttggatgaaa taaaattgct caaatgtgtt cgagaaagtg
481atcccagtga cccaaacaaa gacatggtgg tccagctcat tgacgacttc aagatttcag
541gcatgaatgg gatacatgtc tgcatggtct tcgaagtact tggccaccat ctcctcaagt
601ggatcatcaa atccaactat caaggcctcc cagtacgttg tgtgaagagt atcattcgac
661aggtccttca agggttagat tacttacaca gtaagtgcaa gatcattcat actgacataa
721agccggaaaa tatcttgatg tgtgtggatg atgcatatgt gagaagaatg gcagctgagg
781ccactgagtg gcagaaagca ggtgctcctc ctccttcagg gtctgcagtg agtacggctc
841cacagcagaa acctatagga aaaatatcta aaaacaaaaa gaaaaaactg aaaaagaaac
901agaagaggca ggctgagtta ttggagaagc gcctgcagga gatagaagaa ttggagcgag
961aagctgaaag gaaaataata gaagaaaaca tcacctcagc tgcaccttcc aatgaccagg
1021atggcgaata ctgcccagag gtgaaactaa aaacaacagg attagaggag gcggctgagg
1081cagagactgc aaaggacaat ggtgaagctg aggaccagga agagaaagaa gatgctgaga
1141aagaaaacat tgaaaaagat gaagatgatg tagatcagga acttgcgaac gtagacccta
1201cgtggataga atcacctaaa accaatggcc atattgagaa tggcccattc tcactggagc
1261agcaactgga cgatgaagat gatgatgaag aagactgccc aaatcctgag gaatataatc
1321ttgatgagcc aaatgcagaa agtgattaca catatagcag ctcctatgaa caattcaatg
1381gtgaattgcc aaatggacga cataaaattc ccgagtcaca gttcccagag ttttccacct
1441cgttgttctc tggatcctta gaacctgtgg cctgcggctc tgtgctttct gagggatcac
1501cacttactga gcaagaggag agcagtccat cccatgacag aagcagaacg gtttcagcct
1561ccagtactgg ggatttgcca aaagcaaaaa cccgggcagc tgacttgttg gtgaatcccc
1621tggatccgcg gaatgcagat aaaattagag taaaaattgc tgacctggga aatgcttgtt
1681gggtgcataa acacttcacg gaagacatcc agacgcgtca gtaccgctcc atagaggttt
1741taataggagc ggggtacagc acccctgcgg acatctggag cacggcgtgt atggcatttG
1801AGCTGGCAAC GGGAGATTAT TTGTTTGAAC CACATTCTGG GGAAGACTAT TCCAGAGACG
1861AAGACCACAT AGCCCACATC ATAGAGCTGC TAGGCAGTAT TCCAAGGCAC TTTGCTCTAT
1921CTGGAAAATA TTCTCGGGAA TTCTTCAATC GCAGAGGAGA ACTGCGACAC ATCACCAAGC
1981TGAAGCCCTG GAGCCTCTTT GATGTACTTG TGGAAAAGTA TGGCTGGCCC CATGAAGATG
2041CTGCACAGTT TACAGATTTC CTGATCCCGA TGTTAGAAAT GGTTCCAGAA AAACGAGCCT
2101CAGCTGGCGA ATGCCTTCGG CATCCTTGGT TGAATTCTGA CCCAGCTTTC TTGTACAAAG
2161TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2221GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2281ACGATGGGAC TGCATACCAA AGATACGGAC GCGTTAAGTC gacaatcaac ctctggatta
2341caaaatttgt gaaagatt

Download FASTA (ORF) (Full)