Construct: ORF TRCN0000489416
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019460.1_s317c1
- DNA Barcode:
- TCTGCCCAAAACTGACGATTGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTBK2 (146057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489416
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146057 | TTBK2 | tau tubulin kinase 2 | NM_173500.4 | 99.9% | 99.9% | 3732_3733insG |
| 2 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449849.1 | 99.9% | 99.9% | 3732_3733insG |
| 3 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449850.1 | 99.9% | 99.9% | 3732_3733insG |
| 4 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_005254173.5 | 94.3% | 94.2% | (many diffs) |
| 5 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_006720403.4 | 94.3% | 94.2% | (many diffs) |
| 6 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449851.1 | 94.3% | 94.2% | (many diffs) |
| 7 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_006720402.4 | 92.7% | 92.9% | 1_133del;201_202ins148;3717_3718insG |
| 8 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_017021950.2 | 92.4% | 92.4% | 0_1ins279;3453_3454insG |
| 9 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_005254171.5 | 91.9% | 92.2% | 1_166del;234_235ins148;3750_3751insG |
| 10 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_001024856.2 | 88.8% | 89.7% | (many diffs) |
| 11 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_001024857.2 | 88.8% | 89.7% | (many diffs) |
| 12 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_080788.3 | 84.2% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 3800
- ORF length:
- 3735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaactag 61 taccatgagt gggggaggag agcagctgga tatcctgagt gttggaatcc tagtgaaaga 121 aagatggaaa gtgttgagaa agattggggg tgggggcttt ggagaaattt acgatgcctt 181 ggacatgctc accagggaaa atgttgcact gaaggtggaa tcagctcaac aaccaaaaca 241 agttctgaaa atggaagttg ctgttttgaa aaagctgcaa gggaaagacc atgtttgtag 301 atttattggc tgtgggagga atgatcgatt caactatgtg gtcatgcagt tgcagggtcg 361 gaatctggca gatcttcgcc gtagccagtc ccgaggcaca ttcaccatta gtaccactct 421 ccggctgggt agacagattt tggagtctat tgaaagcatt cattctgtgg gattcttgca 481 tcgagacatc aaaccgtcga acttcgctat gggtcgcttt cctagtacat gtaggaaatg 541 ttacatgctt gattttggct tggctcgaca atttaccaat tcctgtggtg acgtcagacc 601 acctcgagct gtggcaggtt ttcgagggac agttcgttat gcatcaatca acgcacatcg 661 gaacagggaa atgggaagac atgatgacct ttggtcctta ttctacatgt tggtggagtt 721 tgtggttggt cagctgccct ggagaaaaat aaaggacaag gagcaagtag gctctattaa 781 ggagagatat gaccacaggc tcatgttgaa acatctccct ccagaattca gcatctttct 841 agaccatatc tcttctttgg attattttac aaaaccagac taccagcttc ttacatccgt 901 gtttgacaat agcatcaaga cttttggagt aattgagagt gacccttttg actgggagaa 961 gactggaaat gatggctccc taacaaccac cactacttct accacccctc agttgcacac 1021 tcgcttgacc cctgctgcaa ttggaattgc caatgctact cccatccctg gagacttgct 1081 tcgagaaaat acagatgagg tatttccaga tgaacagctt agcgatggag aaaatggcat 1141 ccctgttggt gtgtcaccag ataaattgcc tggatctctg ggacaccccc gtccccagga 1201 gaaggatgtt tgggaagaga tggatgccaa caaaaacaag ataaagcttg gaatttgtaa 1261 ggctgctact gaagaggaga acagccatgg ccaggcaaat ggtcttctca atgctccaag 1321 ccttgggtca ccaattcgtg tccgctcaga gattactcag ccagacagag atattccact 1381 ggtgcgaaag ttacgttcca ttcacagctt tgagctggaa aaacgtctga ccctggagcc 1441 aaagccagac actgacaagt tccttgagac ctgcctggag aaaatgcaga aagataccag 1501 tgcaggaaaa gaatctattc tccctgctct gctgcataag ccttgcgttc ctgctgtgtc 1561 ccgtactgac cacatctggc actatgatga agaatatctt ccagatgcct ccaagcctgc 1621 ttctgccaac acccctgagc aggcagatgg tggtggcagc aatggattta tagctgttaa 1681 cctgagctct tgcaagcaag aaattgattc caaagaatgg gtgattgtgg acaaggagca 1741 ggaccttcag gattttagga caaatgaggc tgtaggacat aaaacaactg gaagtccttc 1801 tgatgaggag cctgaagtac ttcaagtcct ggaggcatca cctcaagatg aaaagctcca 1861 gttaggtcct tgggcagaaa atgatcattt aaagaaggaa acctcaggtg tggtcttagc 1921 actttctgca gagggtcctc ctactgctgc ttcagaacaa tatacagata ggctggaact 1981 ccagcctgga gctgctagtc agtttattgc agcgacgccc acaagtctaa tggaggcgca 2041 ggcagaagga ccccttacag cgattacaat tcctagacct tctgtggcat ctacacagtc 2101 aacttcagga agctttcact gtggtcagca gccagagaag aaagatcttc agcccatgga 2161 gcccactgtg gaactttact ctccaaggga aaacttctct ggcttggttg tgacagaggg 2221 tgaacctcct agtggaggaa gcagaacaga tttggggctt cagatagatc acattggtca 2281 tgacatgtta cccaacatta gagaaagtaa caaatctcaa gacctgggac caaaagaact 2341 tcctgatcat aatagactgg ttgtgagaga atttgaaaat ctccctgggg aaactgaaga 2401 gaaaagcatc cttttagagt cagataatga agatgagaag ttaagtagag ggcagcattg 2461 tattgagatc tcctctctcc caggagattt ggtaattgtg gaaaaggatc actcagctac 2521 tactgaacct cttgatgtga caaaaacaca gacttttagt gtggtgccaa atcaagacaa 2581 aaataatgag ataatgaagc ttctgacagt tggaacttca gaaatttctt ccagagacat 2641 tgacccacat gttgaaggtc agataggcca agtggcagaa atgcaaaaaa ataagatatc 2701 taaggatgat gacatcatga gtgaagactt gccaggtcat caaggagacc tctctacttt 2761 tttgcaccaa gagggcaaga gagagaaaat cacccctaga aatggagaac tatttcattg 2821 tgtttcagag aatgaacatg gtgccccaac ccggaaggat atggttaggt catcctttgt 2881 aactagacac agccgaatcc ctgttttagc acaagagata gactcaactt tggaatcatc 2941 ctctccagtt tctgcaaaag aaaagctcct ccaaaagaaa gcctatcagc cagacctagt 3001 caagcttctg gtggaaaaaa gacaattcaa gtccttcctt ggcgacctct caagtgcctc 3061 tgataaattg ctagaggaga aactagctac tgttcctgct cccttttgtg aggaggaagt 3121 gctcactccc ttttcaagac tgacagtaga ttctcacctg agtaggtcag ctgaagatag 3181 ctttctgtca cccatcatct cccagtctag aaagagcaaa attccaaggc cagtttcatg 3241 ggtcaacaca gatcaggtca atagctcaac ttcgtctcag ttctttcctc ggccaccacc 3301 aggaaagcca cccacgaggc ctggagtaga agccaggcta cgcagatata aagtcctagg 3361 gagtagtaac tccgactcag accttttctc ccgcctggcc caaattcttc aaaatggatc 3421 tcagaaaccc cGGAGCACTA CTCAGTGCAA GAGTCCAGGA TCTCCTCACA ATCCAAAAAC 3481 ACCACCCAAG AGTCCAGTTG TCCCTCGCAG GAGTCCCAGT GCCTCTCCTC GAAGCTCATC 3541 CTTGCCTCGC ACGTCTAGTT CCTCACCATC TAGGGCTGGA CGGCCCCACC ATGACCAGAG 3601 GAGTTCGTCC CCACATCTGG GGAGAAGCAA GTCACCTCCC AGCCACTCAG GATCTTCCTC 3661 CTCCAGGAGG TCCTGCCAAC AGGAGCATTG CAAACCCAGC AAGAATGGCC TGAAAGGATC 3721 CGGCAGCCTC CACCACCACT CAGCCAGCAC TAAAACCCCC CAAGGGAAGA GTAAGCCAGC 3781 CAGTAAACTC AGCAGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 3841 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 3901 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTGCCCA AAACTGACGA 3961 TTGCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt