Construct: ORF TRCN0000489438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020297.1_s317c1
- DNA Barcode:
- TACAAGTCCACCTCAGCGCGCCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LYN (4067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | NM_002350.4 | 99.8% | 99.8% | 1536_1537insTTG |
| 2 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | NM_001111097.3 | 95.7% | 95.7% | 66_67ins63;1473_1474insTTG |
| 3 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | XM_017013416.1 | 95.7% | 95.7% | 66_67ins63;1473_1474insTTG |
| 4 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | XM_011517529.3 | 71.2% | 66.8% | (many diffs) |
| 5 | human | 4067 | LYN | LYN proto-oncogene, Src fam... | XM_017013415.2 | 44.7% | 40.3% | (many diffs) |
| 6 | mouse | 17096 | Lyn | LYN proto-oncogene, Src fam... | NM_001111096.1 | 88.7% | 95.9% | (many diffs) |
| 7 | mouse | 17096 | Lyn | LYN proto-oncogene, Src fam... | NM_010747.2 | 84.7% | 92% | (many diffs) |
| 8 | mouse | 17096 | Lyn | LYN proto-oncogene, Src fam... | XM_011249939.2 | 73% | 80.1% | (many diffs) |
| 9 | mouse | 666513 | Gm11787 | predicted gene 11787 | XM_017320471.2 | 51.3% | 53.6% | (many diffs) |
| 10 | mouse | 108168924 | LOC108168924 | uncharacterized LOC108168924 | XR_001784222.1 | 9.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1611
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggatgt ataaaatcaa aagggaaaga cagcttgagt gacgatggag 121 tagatttgaa gactcaacca gtacgtaata ctgaaagaac tatttatgtg agagatccaa 181 cgtccaataa acagcaaagg ccagttccag aatctcagct tttacctgga cagaggtttc 241 aaactaaaga tccagaggaa caaggagaca ttgtggtagc cttgtacccc tatgatggca 301 tccacccgga cgacttgtct ttcaagaaag gagagaagat gaaagtcctg gaggagcatg 361 gagaatggtg gaaagcaaag tcccttttaa caaaaaaaga aggcttcatc cccagcaact 421 atgtggccaa actcaacacc ttagaaacag aagagtggtt tttcaaggat ataaccagga 481 aggacgcaga aaggcagctt ttggcaccag gaaatagcgc tggagctttc cttattagag 541 aaagtgaaac attaaaagga agcttctctc tgtctgtcag agactttgac cctgtgcatg 601 gtgatgttat taagcactac aaaattagaa gtctggataa tgggggctat tacatctctc 661 cacgaatcac ttttccctgt atcagcgaca tgattaaaca ttaccaaaag caggcagatg 721 gcttgtgcag aagattggag aaggcttgta ttagtcccaa gccacagaag ccatgggata 781 aagatgcctg ggagatcccc cgggagtcca tcaagttggt gaaaaggctt ggcgctgggc 841 agtttgggga agtctggatg ggttactata acaacagtac caaggtggct gtgaaaaccc 901 tgaagccagg aactatgtct gtgcaagcct tcctggaaga agccaacctc atgaagaccc 961 tgcagcatga caagctcgtg aggctctacg ctgtggtcac cagggaggag cccatttaca 1021 tcatcaccga gtacatggcc aagggcagtt tgctggattt cctgaagagc gatgaaggtg 1081 gcaaagtgct gcttccaaag ctcattgact tttctgctca gattgcagag ggaatggcat 1141 acatcgagcg gaagaactac attcaccggg acctgcgagc agctaatgtt ctggtctccg 1201 agtcactcat gtgcaaaatt gcagattttg gCCTTGCTAG AGTAATTGAA GATAATGAGT 1261 ACACAGCAAG GGAAGGTGCT AAGTTCCCTA TTAAGTGGAC GGCTCCAGAA GCAATCAACT 1321 TTGGATGTTT CACTATTAAG TCTGATGTGT GGTCCTTTGG AATCCTCCTA TACGAAATTG 1381 TCACCTATGG GAAAATTCCC TACCCAGGGA GAACTAATGC CGACGTGATG ACCGCCCTGT 1441 CCCAGGGCTA CAGGATGCCC CGTGTGGAGA ACTGCCCAGA TGAGCTCTAT GACATTATGA 1501 AAATGTGCTG GAAAGAAAAG GCAGAAGAGA GACCAACGTT TGACTACTTA CAGAGCGTCC 1561 TGGATGATTT CTACACAGCC ACGGAAGGGC AATACCAGCA GCAGCCTTTG TGAGACCCAG 1621 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1681 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1741 TATCTTGTGG AAAGGACGAT ACAAGTCCAC CTCAGCGCGC CTCACGCGTT AAGTCgacaa 1801 tcaacctctg gattacaaaa tttgtgaaag att