Construct: ORF TRCN0000489458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021445.1_s317c1
- DNA Barcode:
- GCCGTTAGCATGGAGTTATCGAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ACSBG1 (23205)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489458
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23205 | ACSBG1 | acyl-CoA synthetase bubbleg... | NM_015162.5 | 99.5% | 100% | 1474C>A;2172_2173insTAAAAGCT |
| 2 | human | 23205 | ACSBG1 | acyl-CoA synthetase bubbleg... | NM_001199377.2 | 99% | 99.4% | 446_447insCCTGAAGCTCGG;1462C>A;2160_2161insTAAAAGCT |
| 3 | human | 23205 | ACSBG1 | acyl-CoA synthetase bubbleg... | XM_017022025.2 | 95.8% | 95.3% | (many diffs) |
| 4 | human | 23205 | ACSBG1 | acyl-CoA synthetase bubbleg... | XM_011521390.3 | 95.7% | 92.7% | (many diffs) |
| 5 | human | 23205 | ACSBG1 | acyl-CoA synthetase bubbleg... | XM_017022026.1 | 95.2% | 92.7% | (many diffs) |
| 6 | human | 23205 | ACSBG1 | acyl-CoA synthetase bubbleg... | XM_011521391.2 | 86% | 85.2% | (many diffs) |
| 7 | mouse | 94180 | Acsbg1 | acyl-CoA synthetase bubbleg... | NM_053178.2 | 84% | 86.1% | (many diffs) |
| 8 | mouse | 94180 | Acsbg1 | acyl-CoA synthetase bubbleg... | XM_006511588.2 | 82.2% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2238
- ORF length:
- 2172
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatgcc acgcaattct ggagctggat acggctgccc acacggggac cccagcatgc 121 tggacagcag agagacccca caggagagcc ggcaggacat gattgtgagg accacccaag 181 aaaaattgaa aaccagctca ctgactgaca ggcagccact ctccaaagag tccctgaacc 241 atgctctcga gctctcagtg ccagagaagg tgaataatgc ccagtgggat gctccagagg 301 aggcgctgtg gacgactcgg gccgatgggc gggtgcgcct gcgcatagac cccagctgcc 361 cacagcttcc ctacactgtg catcggatgt tctacgaggc cctggataag tatggggacc 421 tcatcgcttt gggcttcaag cgccaggaca agtgggaaca catctcctac tcccaatact 481 acctgctcgc ccgcagagcc gccaagggct tcctgaagct cggcctgaag caggcccaca 541 gtgtggccat cctcggcttc aactccccgg agtggttctt ctcggcagtg ggcacagtat 601 ttgcaggtgg catcgtcact ggcatctaca ccaccagctc cccagaggcc tgccagtaca 661 tcgcttatga ctgctgcgcc aatgtcatca tggtcgacac gcagaagcag ctggaaaaga 721 tcctgaagat ctggaaacag ttgccacatc taaaggcagt cgtgatatat aaagaacctc 781 ctccaaacaa gatggccaat gtgtacacga tggaggaatt catggagctg gggaatgaag 841 tgcctgagga agccctggac gccatcattg acacccagca gcccaaccag tgctgtgtgc 901 tagtctacac ttccggcacc actgggaacc ccaagggcgt gatgctgagt caagacaata 961 tcacgtggac ggcacggtac ggcagccagg ccggtgacat ccggccggca gaagtccagc 1021 aggaggtggt agtcagctac ctgcccctca gccatattgc cgcccagatc tacgacctgt 1081 ggacaggcat ccagtggggg gcccaggttt gctttgccga acccgacgcc ctgaagggga 1141 gcctggtgaa cacgctgcgg gaggtggagc ccacatcaca catgggggtg ccccgggtat 1201 gggagaagat catggagcgc atccaggagg tggcggctca gtctggcttc atccggcgaa 1261 agatgctgct gtgggccatg tcggtgacct tggagcagaa cctcacctgc cccggcagcg 1321 acctgaagcc cttcacaacc agactggcag attacctggt gctagccaag gttcgccagg 1381 cactgggatt tgccaagtgt caaaagaact tctatggagc ggcccccatg atggcagaga 1441 cacagcactt cttcctgggt ctcaacatcc gcttgtatgc gggctacggc ctcagtgaga 1501 cctcaggccc ccacttcatg tccagtccct acaactacag gctgtacagc tcaggcaagt 1561 tggtgcccgg ctgtcgggtg aagctggtga accaggacgc agagggcatt ggcgagatct 1621 gcctgtgggg ccgcaccata ttcatgggct acctgaacat ggaggacaag acttgtgagg 1681 ccatcgacga ggaaggctgg ctgcacacgg gtgatgctgg ccgcctggac gccgatggct 1741 tcctctacat cactgggcgc ctcaaagaat taatcatcac agctggtggg gagaatgtgc 1801 cccctgtgcc catcgaggag gccgtgaaga tGGAGCTGCC CATCATCAGC AACGCCATGC 1861 TCATTGGGGA CCAGAGGAAG TTCCTGTCCA TGCTGCTCAC CTTGAAGTGC ACTCTGGACC 1921 CAGACACCTC TGACCAGACT GATAATCTGA CTGAACAAGC TATGGAGTTC TGCCAGAGGG 1981 TGGGCAGCAG AGCCACCACA GTGTCCGAGA TCATAGAGAA GAAGGATGAG GCCGTGTACC 2041 AGGCCATCGA AGAGGGGATC CGGAGGGTCA ACATGAACGC GGCGGCCCGG CCCTACCACA 2101 TCCAGAAGTG GGCCATTCTC GAGAGAGACT TCTCCATTTC GGGTGGAGAG TTGGGTCCCA 2161 CGATGAAACT GAAACGGCTC ACAGTTTTGG AGAAGTACAA AGGTATCATT GACTCCTTTT 2221 ACCAAGAGCA AAAAATGTAA AAGCTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2281 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2341 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCGTTAG 2401 CATGGAGTTA TCGAAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2461 aagatt