Construct: ORF TRCN0000489475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019927.1_s317c1
- DNA Barcode:
- TTACTCACCAATTCGGAAGAAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K7 (6885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145333.3 | 99.9% | 99.7% | 1473_1474insG |
| 2 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145332.3 | 94.7% | 94.6% | 1208_1288del;1554_1555insG |
| 3 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_003188.4 | 84.8% | 82.9% | 1444_1559del;1590_1737delinsG |
| 4 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145331.3 | 81% | 79.3% | 1208_1288del;1525_1640del;1671_1818delinsG |
| 5 | human | 6885 | MAP3K7 | mitogen-activated protein k... | XM_017011226.2 | 62.3% | 60.6% | 0_1ins390;1054_1169del;1200_1347delinsG |
| 6 | human | 6885 | MAP3K7 | mitogen-activated protein k... | XM_006715553.3 | 59.5% | 57.9% | (many diffs) |
| 7 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | NM_172688.3 | 77.2% | 82.3% | (many diffs) |
| 8 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | NM_009316.1 | 74% | 78.6% | (many diffs) |
| 9 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | XM_006537948.3 | 54.7% | 57.4% | (many diffs) |
| 10 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | XR_390323.3 | 20.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1545
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtctacagcc tctgccgcct cctcctcctc ctcgtcttcg gccggtgaga 121 tgatcgaagc cccttcccag gtcctcaact ttgaagagat cgactacaag gagatcgagg 181 tggaagaggt tgttggaaga ggagcctttg gagttgtttg caaagctaag tggagagcaa 241 aagatgttgc tattaaacaa atagaaagtg aatctgagag gaaagcgttt attgtagagc 301 ttcggcagtt atcccgtgtg aaccatccta atattgtaaa gctttatgga gcctgcttga 361 atccagtgtg tcttgtgatg gaatatgctg aagggggctc tttatataat gtgctgcatg 421 gtgctgaacc attgccatat tatactgctg cccacgcaat gagttggtgt ttacagtgtt 481 cccaaggagt ggcttatctt cacagcatgc aacccaaagc gctaattcac agggacctga 541 aaccaccaaa cttactgctg gttgcagggg ggacagttct aaaaatttgt gattttggta 601 cagcctgtga cattcagaca cacatgacca ataacaaggg gagtgctgct tggatggcac 661 ctgaagtttt tgaaggtagt aattacagtg aaaaatgtga cgtcttcagc tggggtatta 721 ttctttggga agtgataacg cgtcggaaac cctttgatga gattggtggc ccagctttcc 781 gaatcatgtg ggctgttcat aatggtactc gaccaccact gataaaaaat ttacctaagc 841 ccattgagag cctgatgact cgttgttggt ctaaagatcc ttcccagcgc ccttcaatgg 901 aggaaattgt gaaaataatg actcacttga tgcggtactt tccaggagca gatgagccat 961 tacagtatcc ttgtcagtat tcagatgaag gacagagcaa ctctgccacc agtacaggct 1021 cattcatgga cattgcttct acaaatacga gtaacaaaag tgacactaat atggagcaag 1081 ttcctgccac aaatgatact attaagcgct tagaatcaaa attgttgaaa aatcaggcaa 1141 agcaacagag tgaatctgga cgtttaagct tGGGAGCCTC CCGTGGGAGC AGTGTGGAGA 1201 GCTTGCCCCC AACCTCTGAG GGCAAGAGGA TGAGTGCTGA CATGTCTGAA ATAGAAGCTA 1261 GGATCGCCGC AACCACAGGC AACGGACAGC CAAGACGTAG ATCCATCCAA GACTTGACTG 1321 TAACTGGAAC AGAACCTGGT CAGGTGAGCA GTAGGTCATC CAGTCCCAGT GTCAGAATGA 1381 TTACTACCTC AGGACCAACC TCAGAAAAGC CAACTCGAAG TCATCCATGG ACCCCTGATG 1441 ATTCCACAGA TACCAATGGA TCAGATAACT CCATCCCAAT GGCTTATCTT ACACTGGATC 1501 ACCAACTACA GGCAAGAACT AGTTGCAGAA CTGGACCAGG AGACCCAGCT TTCTTGTACA 1561 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1621 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGATTA CTCACCAATT CGGAAGAAGG TACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t