Construct: ORF TRCN0000489481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019309.1_s317c1
- DNA Barcode:
- GAACTCGCCCCACTTAAGATGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR153 (387509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387509 | GPR153 | G protein-coupled receptor 153 | NM_207370.4 | 99.9% | 99.8% | 1827_1828insG |
| 2 | human | 387509 | GPR153 | G protein-coupled receptor 153 | XM_011541434.3 | 99.9% | 99.8% | 1827_1828insG |
| 3 | human | 387509 | GPR153 | G protein-coupled receptor 153 | XM_017001250.1 | 99.9% | 99.8% | 1827_1828insG |
| 4 | human | 387509 | GPR153 | G protein-coupled receptor 153 | XM_017001251.2 | 71.1% | 66.1% | (many diffs) |
| 5 | mouse | 100129 | Gpr153 | G protein-coupled receptor 153 | XM_011250169.1 | 84% | 87.5% | (many diffs) |
| 6 | mouse | 100129 | Gpr153 | G protein-coupled receptor 153 | NM_178406.2 | 81% | 84.3% | (many diffs) |
| 7 | mouse | 100129 | Gpr153 | G protein-coupled receptor 153 | XM_011250170.1 | 72.8% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1902
- ORF length:
- 1830
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagtgat gagcggcggc tgcctggcag tgcagtgggc tggctggtat 121 gtgggggcct ctccctgctg gccaatgcct ggggcatcct cagcgttggc gccaagcaga 181 agaagtggaa gcccttggag ttcctgctgt gtacactcgc ggccacccac atgctaaatg 241 tggccgtgcc catcgccacc tactccgtgg tgcagctgcg gcggcagcgc cccgacttcg 301 agtggaatga gggtctctgc aaggtcttcg tgtccacctt ctacaccctc accctggcca 361 cctgtttctc tgtcacctcc ctctcctacc accgcatgtg gatggtctgc tggcctgtca 421 actaccggct gagcaatgcc aagaagcagg cggtgcacac agtcatgggt atctggatgg 481 tgtccttcat cctgtcggcc ctgcctgccg ttggctggca cgacaccagc gagcgcttct 541 acacccatgg ctgccgcttc atcgtggctg agatcggcct gggctttggc gtctgcttcc 601 tgctgctggt gggcggcagc gtggccatgg gcgtgatctg cacagccatc gccctcttcc 661 agacgctggc cgtgcaggtg gggcgccagg ccgaccgccg cgccttcacc gtgcccacca 721 tcgtggtgga ggacgcgcag ggcaagcggc gctcctccat cgatggctcg gagcccgcca 781 aaacctctct gcagaccacg ggcctcgtga ccaccatagt cttcatctac gactgcctca 841 tgggcttccc tgtgctggtg gtgagcttca gcagcctgcg ggccgacgcc tcagcgccct 901 ggatggcact ctgcgtgctg tggtgctccg tggcccaggc cctgctgctg cctgtgttcc 961 tctgggcctg cgaccgctac cgggctgacc tcaaagctgt ccgggagaag tgcatggccc 1021 tcatggccaa cgacgaggag tcagacgatg agaccagcct ggaaggtggc atctccccgg 1081 acctggtgtt ggagcgctcc ctggactatg gctatggagg tgattttgtg gccctagata 1141 ggatggccaa gtatgagatc tccgccctgg aggggggcct gccccagctc tacccactgc 1201 ggcccttgca ggaggacaag atgcaatacc tgcaggtccc gcccacgcgg cgcttctccc 1261 acgacgatgc ggacgtgtgg gccgccgtcc cgctgcccgc cttcctgccg cgctggggct 1321 ccggcgagga cctggccgcc ctggcgcacc tggtgctgcc tgccgggccc gagcggcgcc 1381 gcgccagcct cctggccttc gcggaggacg caccaccgtc ccgcgcgcgc cgccgctcgg 1441 ccgagagcct gctgtcgctg cggccctcgg ccctggatag cggcccgcgg ggagcccgcg 1501 actcgccccc cggcagcccg cgccgccgcc ccgggcccgg cccccgctcc gcctcggcct 1561 cgctgctgcc cgacgccttc gccctgaccg ccttcgagtg cgagccacag gccctgcgcc 1621 gcccgcccgg gcccttcccc gctgcgcccg ccgcccccga cggcgcagat cccggAGAGG 1681 CCCCGACGCC CCCAAGCAGC GCCCAGCGGA GCCCAGGGCC ACGCCCCTCT GCGCACTCGC 1741 ACGCCGGCTC TCTGCGCCCC GGCCTGAGCG CGTCGTGGGG CGAGCCCGGG GGGCTGCGCG 1801 CGGCGGGCGG CGGCGGCAGC ACCAGCAGCT TCCTGAGTTC CCCCTCCGAG TCCTCGGGCT 1861 ACGCCACGCT GCACTCGGAC TCGCTGGGCT CCGCGTCCGA CCCAGCTTTC TTGTACAAAG 1921 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1981 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2041 ACGAGAACTC GCCCCACTTA AGATGCCCAC GCGTTAAGTC gacaatcaac ctctggatta 2101 caaaatttgt gaaagatt