Transcript: Human XM_017001251.2

PREDICTED: Homo sapiens G protein-coupled receptor 153 (GPR153), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GPR153 (387509)
Length:
1978
CDS:
252..1616

Additional Resources:

NCBI RefSeq record:
XM_017001251.2
NBCI Gene record:
GPR153 (387509)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001251.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359823 TAGATAGGATGGCCAAGTATG pLKO_005 1315 CDS 100% 10.800 15.120 N GPR153 n/a
2 TRCN0000063439 CGTGACCACCATAGTCTTCAT pLKO.1 986 CDS 100% 4.950 6.930 N GPR153 n/a
3 TRCN0000063440 CTATGGCTATGGAGGTGATTT pLKO.1 1286 CDS 100% 13.200 9.240 N GPR153 n/a
4 TRCN0000359822 CAGGAGGACAAGATGCAATAC pLKO_005 1389 CDS 100% 10.800 7.560 N GPR153 n/a
5 TRCN0000063442 CCTAGATAGGATGGCCAAGTA pLKO.1 1313 CDS 100% 4.950 3.465 N GPR153 n/a
6 TRCN0000359821 TCGTGACCACCATAGTCTTCA pLKO_005 985 CDS 100% 4.950 3.465 N GPR153 n/a
7 TRCN0000063441 CGCCAAGCAGAAGAAGTGGAA pLKO.1 350 CDS 100% 2.640 1.848 N GPR153 n/a
8 TRCN0000063438 GCAGGAGGACAAGATGCAATA pLKO.1 1388 CDS 100% 10.800 6.480 N GPR153 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001251.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489481 GAACTCGCCCCACTTAAGATGCCC pLX_317 12.6% 71.1% 66.1% V5 (many diffs) n/a
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