Construct: ORF TRCN0000489489

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021228.1_s317c1
DNA Barcode:
GGGATAACATGATGAAAACTTGCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADGRG2 (10149)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489489
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001079859.3 99.9% 100% 1248T>C
2 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001079860.3 99.1% 99.2% 201_202ins24;1224T>C
3 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001079858.3 98.5% 98.6% 260_301del;1290T>C
4 human 10149 ADGRG2 adhesion G protein-coupled ... XM_006724455.3 98.5% 98.6% 260_301del;1290T>C
5 human 10149 ADGRG2 adhesion G protein-coupled ... XM_011545434.1 98.5% 98.6% 260_301del;1290T>C
6 human 10149 ADGRG2 adhesion G protein-coupled ... XM_011545435.2 98.5% 98.6% 260_301del;1290T>C
7 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001184837.2 98.3% 98.4% 153_154ins48;1200T>C
8 human 10149 ADGRG2 adhesion G protein-coupled ... NM_005756.4 98.2% 98.3% 192_193insGGTACTCCA;251_292del;1281T>C
9 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001184835.2 97.5% 97.6% 152_153ins72;1176T>C
10 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001184833.1 97% 97% 153_154ins48;212_253del;1242T>C
11 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001184836.1 96.2% 96.2% 152_153ins72;188_229del;1218T>C
12 human 10149 ADGRG2 adhesion G protein-coupled ... NM_001184834.2 93.5% 93.6% 260_301del;1290T>C;2714_2715ins153
13 mouse 237175 Adgrg2 adhesion G protein-coupled ... NM_001079857.2 83% 80.5% (many diffs)
14 mouse 237175 Adgrg2 adhesion G protein-coupled ... NM_001079847.2 82.7% 80.2% (many diffs)
15 mouse 237175 Adgrg2 adhesion G protein-coupled ... NM_001290446.1 82.7% 80% (many diffs)
16 mouse 237175 Adgrg2 adhesion G protein-coupled ... NM_178712.4 82.5% 80% (many diffs)
17 mouse 237175 Adgrg2 adhesion G protein-coupled ... NM_001290445.1 82.2% 79.7% (many diffs)
18 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528824.3 82% 79.5% (many diffs)
19 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_011247817.2 82% 79.5% (many diffs)
20 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_017318479.1 82% 79.5% (many diffs)
21 mouse 237175 Adgrg2 adhesion G protein-coupled ... NM_001079848.2 81.5% 79.2% (many diffs)
22 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528829.2 81% 78.6% (many diffs)
23 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528812.2 77.2% 74.8% (many diffs)
24 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528813.2 77% 74.5% (many diffs)
25 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528808.2 76.8% 74.4% (many diffs)
26 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528810.2 76.7% 74.3% (many diffs)
27 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528811.2 76.5% 74.1% (many diffs)
28 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528806.2 76.3% 73.9% (many diffs)
29 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528816.2 75.9% 73.6% (many diffs)
30 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528814.2 75.4% 73.1% (many diffs)
31 mouse 237175 Adgrg2 adhesion G protein-coupled ... XM_006528819.2 75% 73.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3081
ORF length:
3009
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggttttc tctgtcaggc agtgtggcca tgttggcaga actgaagaag
121ttttactgac gttcaagata ttccttgtca tcatttgtct tcatgtcgtt ctggtaacat
181ccctggaaga agatactgat aattccagtt tgtcaccacc acctgctaaa ttatctgttg
241tcagttttgc cccctcctcc aatggtactc cagaggttga aacaacaagc ctcaatgatg
301ttactttaag cttactccct tcaaacgaaa caggcgtcaa accccagaga aatatctgca
361atttgtcatc tatttgcaat gactcagcat tttttagagg tgagatcatg tttcaatatg
421ataaagaaag cactgttccc cagaatcaac atataacgaa tggcacctta actggagtcc
481tgtctctaag tgaattaaaa cgctcagagc tcaacaaaac cctgcaaacc ctaagtgaga
541cttactttat aatgtgtgct acagcagagg cccaaagcac attaaattgt acattcacaa
601taaaactgaa taatacaatg aatgcatgtg ctgtaatagc tgctttggaa agagtaaaga
661ttcgaccaat ggaacactgc tgctgttctg tcaggatacc ctgcccttcc tccccagaag
721agttggaaaa gcttcagtgt gacctgcagg atcccattgt ctgtcttgct gaccatccac
781gtggcccacc attttcttcc agccaatcca tcccagtggt gcctcgggcc actgtgcttt
841cccaggtccc caaagctacc tcttttgctg agcctccaga ttattcacct gtgacccaca
901atgttccctc tccaataggg gagattcaac ccctttcacc ccagccttca gctcccatag
961cttccagccc tgccattgac atgcccccac agtctgaaac gatctcttcc cctatgcccc
1021aaacccatgt ctccggcacc ccacctcctg tgaaagcctc attttcctct cccaccgtgt
1081ctgcccctgc gaatgtcaac actaccagcg cacctcctgt ccagacagac atcgtcaaca
1141ccagcagtat ttctgatctt gagaaccaag tgttgcagat ggagaaggct ctgtccttgg
1201gcagcctgga gcctaacctc gcaggagaaa tgatcaacca agtcagcaga ctccttcatt
1261ccccgcctga catgctggcc cctctggctc aaagattgct gaaagtagtg gatgacatcg
1321gcctacagct gaacttttca aacacgacta taagtctaac ctccccttct ttggctctgg
1381ctgtgatcag agtgaatgcc agtagtttca acacaactac ctttgtggcc caagaccctg
1441caaatcttca ggtttctctg gaaacccaag ctcctgagaa cagtattggc acaattactc
1501ttccttcatc gctgatgaat aatttaccag ctcatgacat ggagctagct tccagggttc
1561agttcaattt ttttgaaaca cctgctttgt ttcaggatcc ttccctggag aacctctctc
1621tgatcagcta cgtcatatca tcgagtgttg caaacctgac cgtcaggaac ttgacaagaa
1681acgtgacagt cacattaaag cacatcaacc cgagccagga tgagttaaca gtgagatgtg
1741tattttggga cttgggcaga aatggtggca gaggaggctg gtcagacaat ggctgctctg
1801tcaaagacag gagattgaat gaaaccatct gtacctgtag ccatctaaca agcttcggcg
1861ttctgctgga cctatctagg acatctgtgc tgcctgctca aatgatggct ctgacgttca
1921ttacatatat tggttgtggg ctttcatcaa tttttctgtc agtgactctt gtaacctaca
1981tagcttttga aaagatccgg agggattacc cttccaaaat cctcatccag ctgtgtgctg
2041ctctgcttct gctgaacctg gtcttcctcc tggactcgtg gattgctctg tataagatgc
2101aaggcctctg catctcagtg gctgtatttc ttcattattt tctcttggtc tcattcacat
2161ggatgggcct agaagcattc catatgtacc tggcccttgt caaagtattt aatacttaca
2221tccgaaaata catccttaaa ttctgcattg tcggttgggg ggtaccagct gtggttgtga
2281ccatcatcct gactatatcc ccagataact atgggcttgg atcctatggg aaattcccca
2341atggttcacc ggatgacttc tgctggatca acaacaatgc agtattctac attacggtgg
2401tgggatattt ctgtgtgata tttttgctga acgtcagcat gttcattgtg gtcctggttc
2461agctctgtcg aattaaaaag aagaagcaac tgggagccca gcgaaaaacc agtattcaag
2521acctcaggag tatcgctggc cttacatttt tactgggaat aacttggggc tttgccttct
2581ttgcctgggg accagttaac gtgaccttca tgtatctgtt tgccatcttt aataccttac
2641aaggattttt catattcatc ttttactgtg tggccaaaga aaatgtcagg aagcaatgga
2701ggcggtatct ttgttgtgga aagttacggc tGGCTGAAAA TTCTGACTGG AGTAAAACTG
2761CTACTAATGG TTTAAAGAAG CAGACTGTAA ACCAAGGAGT GTCCAGCTCT TCAAATTCCT
2821TACAGTCAAG CAGTAACTCC ACTAACTCCA CCACACTGCT AGTGAATAAT GATTGCTCAG
2881TACACGCAAG CGGGAATGGA AATGCTTCTA CAGAGAGGAA TGGGGTCTCT TTTAGTGTTC
2941AGAATGGAGA TGTGTGCCTT CACGATTTCA CTGGAAAACA GCACATGTTT AACGAGAAGG
3001AAGATTCCTG CAATGGGAAA GGCCGTATGG CTCTCAGAAG GACTTCAAAG CGGGGAAGCT
3061TACACTTTAT TGAGCAAATG TGAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
3121AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
3181TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GGATAACATG
3241ATGAAAACTT GCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
3301att

Download FASTA (ORF) (Full)