Construct: ORF TRCN0000489544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021862.1_s317c1
- DNA Barcode:
- CCGGAACAGGCACACAGATTTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KYAT3 (56267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001008661.3 | 100% | 100% | |
| 2 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349448.1 | 100% | 100% | |
| 3 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001008662.2 | 92.5% | 92.5% | 0_1ins102 |
| 4 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349447.1 | 92.5% | 92.5% | 0_1ins102 |
| 5 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NR_146183.1 | 75.2% | 1_165del;1528_1809del | |
| 6 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NR_146185.1 | 63.8% | (many diffs) | |
| 7 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349449.1 | 63.8% | 63.8% | 0_1ins492 |
| 8 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349450.1 | 63.8% | 63.8% | 0_1ins492 |
| 9 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NR_146184.1 | 57.6% | 1_377del;1041_1042ins121;1619_2031del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1431
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtttttggcc cagaggagcc tctgctctct tagcggtaga gcaaaattcc 121 tgaagacaat ttcttcttcc aaaatcctcg gattctctac ttctgctaaa atgtcactga 181 aattcacaaa tgcaaaacgg attgaaggac ttgatagtaa tgtgtggatt gaatttacca 241 aattggctgc agacccttct gttgtgaatc ttggccaagg ctttccagat atatcccctc 301 ctacatatgt aaaagaagaa ttatcaaaga ttgcagcaat cgatagcctg aatcagtata 361 cacgaggctt tggccatcca tcacttgtga aagctctgtc ctatctgtat gaaaagcttt 421 atcaaaagca aattgattca aataaagaaa tccttgtgac agtaggagca tatggatctc 481 tttttaacac cattcaagca ttaattgatg agggagatga agtcatacta atagtgcctt 541 tctatgactg ctatgagccc atggtgagaa tggctggagc aacacctgtt tttattcccc 601 tgagatctaa acctgtttat ggaaaaagat ggtctagttc tgactggaca ttagatcctc 661 aagaactgga aagtaaattt aattccaaaa ccaaagctat tatactaaat actccacata 721 acccacttgg caaggtgtat aacagagagg aactgcaagt aattgctgac ctttgcatca 781 aatatgacac actctgcatc agcgatgagg tttatgaatg gcttgtatat tctggaaata 841 agcacttaaa aatagctact tttccaggta tgtgggagag aacaataaca ataggaagtg 901 ctggaaagac tttcagtgta actggctgga agcttggctg gtccattggt ccaaatcatt 961 tgataaaaca tttacagaca gttcaacaaa acacgattta tacttgtgca actcctttac 1021 aggaagcctt ggctcaagct ttctggattg acatcaagcg catggatgac ccagaatgtt 1081 actttaattc tttgccaaaa gagttagaag taaaaagaga tcggatggta cgtttacttg 1141 aaagtgttgg cctaaaaccc atagttcctg atGGAGGATA CTTCATCATC GCTGATGTGT 1201 CTTTGCTAGA TCCAGACCTC TCTGATATGA AGAATAATGA GCCTTATGAC TATAAGTTTG 1261 TGAAATGGAT GACTAAACAT AAGAAACTAT CAGCCATCCC CGTTTCAGCA TTCTGTAACT 1321 CAGAGACTAA ATCACAGTTT GAGAAGTTTG TGCGTTTTTG CTTCATTAAA AAAGACAGCA 1381 CACTGGATGC TGCTGAAGAA ATCATCAAGG CATGGAGTGT ACAGAAGTCT TAGAACCCAG 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAC CGGAACAGGC ACACAGATTT TTAACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att