Transcript: Human NM_001349447.1

Homo sapiens kynurenine aminotransferase 3 (KYAT3), transcript variant 3, mRNA.

Source:
NCBI, updated 2018-06-15
Taxon:
Homo sapiens (human)
Gene:
KYAT3 (56267)
Length:
1914
CDS:
242..1504

Additional Resources:

NCBI RefSeq record:
NM_001349447.1
NBCI Gene record:
KYAT3 (56267)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001349447.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000150970 CAAAGATTGCAGCAATCGATA pLKO.1 396 CDS 100% 4.950 6.930 N KYAT3 n/a
2 TRCN0000151085 GTTTGAGAAGTTTGTGCGTTT pLKO.1 1408 CDS 100% 4.050 5.670 N KYAT3 n/a
3 TRCN0000120415 GAGCCTTATGACTATAAGTTT pLKO.1 1310 CDS 100% 0.563 0.788 N Kyat3 n/a
4 TRCN0000320077 GAGCCTTATGACTATAAGTTT pLKO_005 1310 CDS 100% 0.563 0.788 N Kyat3 n/a
5 TRCN0000150908 GCTGACCTTTGCATCAAATAT pLKO.1 836 CDS 100% 15.000 12.000 N KYAT3 n/a
6 TRCN0000152160 CCTCAAGAACTGGAAAGTAAA pLKO.1 728 CDS 100% 13.200 9.240 N KYAT3 n/a
7 TRCN0000152183 CCTGAGATCTAAACCTGTTTA pLKO.1 670 CDS 100% 13.200 9.240 N KYAT3 n/a
8 TRCN0000155189 GCATGGATGACCCAGAATGTT pLKO.1 1131 CDS 100% 5.625 3.938 N KYAT3 n/a
9 TRCN0000152839 GCTCTGTCCTATCTGTATGAA pLKO.1 464 CDS 100% 5.625 3.938 N KYAT3 n/a
10 TRCN0000151086 GAGATGTTTAACCTCTCAGTA pLKO.1 1761 3UTR 100% 4.950 3.465 N KYAT3 n/a
11 TRCN0000151327 GTCAGCATAACATAGTGGATA pLKO.1 1732 3UTR 100% 4.950 3.465 N KYAT3 n/a
12 TRCN0000150347 CAAATATGACACACTCTGCAT pLKO.1 850 CDS 100% 2.640 1.848 N KYAT3 n/a
13 TRCN0000151215 GATGAGGTTTATGAATGGCTT pLKO.1 875 CDS 100% 2.640 1.848 N KYAT3 n/a
14 TRCN0000155218 GCAGCAATCGATAGCCTGAAT pLKO.1 404 CDS 100% 4.950 2.970 N KYAT3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001349447.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489544 CCGGAACAGGCACACAGATTTTTA pLX_317 19.5% 92.5% 92.5% V5 (not translated due to prior stop codon) 0_1ins102 n/a
2 ccsbBroadEn_12300 pDONR223 100% 69% 69% None 1_390del n/a
3 ccsbBroad304_12300 pLX_304 0% 69% 69% V5 1_390del n/a
4 TRCN0000467583 TTTAGCTTGTCCCACATATTATTA pLX_317 44% 69% 69% V5 1_390del n/a
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