Construct: ORF TRCN0000489572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020813.1_s317c1
- DNA Barcode:
- TATCCTGTTATTGTAACTTCGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LPAR3 (23566)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | NM_012152.3 | 100% | 100% | |
| 2 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | XM_024446128.1 | 100% | 100% | |
| 3 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | XM_024446127.1 | 96.9% | 96.9% | 1_33del |
| 4 | mouse | 65086 | Lpar3 | lysophosphatidic acid recep... | NM_022983.4 | 84.6% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1131
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatgag tgtcactatg acaagcacat ggactttttt tataatagga 121 gcaacactga tactgtcgat gactggacag gaacaaagct tgtgattgtt ttgtgtgttg 181 ggacgttttt ctgcctgttt attttttttt ctaattctct ggtcatcgcg gcagtgatca 241 aaaacagaaa atttcatttc cccttctact acctgttggc taatttagct gctgccgatt 301 tcttcgctgg aattgcctat gtattcctga tgtttaacac aggcccagtt tcaaaaactt 361 tgactgtcaa ccgctggttt ctccgtcagg ggcttctgga cagtagcttg actgcttccc 421 tcaccaactt gctggttatc gccgtggaga ggcacatgtc aatcatgagg atgcgggtcc 481 atagcaacct gaccaaaaag agggtgacac tgctcatttt gcttgtctgg gccatcgcca 541 tttttatggg ggcggtcccc acactgggct ggaattgcct ctgcaacatc tctgcctgct 601 cttccctggc ccccatttac agcaggagtt accttgtttt ctggacagtg tccaacctca 661 tggccttcct catcatggtt gtggtgtacc tgcggatcta cgtgtacgtc aagaggaaaa 721 ccaacgtctt GTCTCCGCAT ACAAGTGGGT CCATCAGCCG CCGGAGGACA CCCATGAAGC 781 TAATGAAGAC GGTGATGACT GTCTTAGGGG CGTTTGTGGT ATGCTGGACC CCGGGCCTGG 841 TGGTTCTGCT CCTCGACGGC CTGAACTGCA GGCAGTGTGG CGTGCAGCAT GTGAAAAGGT 901 GGTTCCTGCT GCTGGCGCTG CTCAACTCCG TCGTGAACCC CATCATCTAC TCCTACAAGG 961 ACGAGGACAT GTATGGCACC ATGAAGAAGA TGATCTGCTG CTTCTCTCAG GAGAACCCAG 1021 AGAGGCGTCC CTCTCGCATC CCCTCCACAG TCCTCAGCAG GAGTGACACA GGCAGCCAGT 1081 ACATAGAGGA TAGTATTAGC CAAGGTGCAG TCTGCAATAA AAGCACTTCC TAGGACCCAG 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAT ATCCTGTTAT TGTAACTTCG CACACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att