Construct: ORF TRCN0000489612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020728.1_s317c1
- DNA Barcode:
- CGATTCCTAACACCTTCTAACGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ABL1 (25)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25 | ABL1 | ABL proto-oncogene 1, non-r... | NM_005157.3:c.944C>T | 99.9% | 100% | 3324A>G |
| 2 | human | 25 | ABL1 | ABL proto-oncogene 1, non-r... | NM_005157.6 | 99.9% | 99.9% | 944C>T;3324A>G |
| 3 | human | 25 | ABL1 | ABL proto-oncogene 1, non-r... | NM_007313.2 | 97.6% | 96.4% | (many diffs) |
| 4 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_009594.4 | 84.2% | 87.6% | (many diffs) |
| 5 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001283045.1 | 83% | 84.7% | (many diffs) |
| 6 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001283046.1 | 82.9% | 85.3% | (many diffs) |
| 7 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001112703.2 | 82.4% | 84.6% | (many diffs) |
| 8 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001283047.1 | 65.5% | 66.9% | (many diffs) |
| 9 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | XM_006497621.3 | 44.3% | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3462
- ORF length:
- 3390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttggag atctgcctga agctggtggg ctgcaaatcc aagaaggggc 121 tgtcctcgtc ctccagctgt tatctggaag aagcccttca gcggccagta gcatctgact 181 ttgagcctca gggtctgagt gaagccgctc gttggaactc caaggaaaac cttctcgctg 241 gacccagtga aaatgacccc aaccttttcg ttgcactgta tgattttgtg gccagtggag 301 ataacactct aagcataact aaaggtgaaa agctccgggt cttaggctat aatcacaatg 361 gggaatggtg tgaagcccaa accaaaaatg gccaaggctg ggtcccaagc aactacatca 421 cgccagtcaa cagtctggag aaacactcct ggtaccatgg gcctgtgtcc cgcaatgccg 481 ctgagtatct gctgagcagc gggatcaatg gcagcttctt ggtgcgtgag agtgagagca 541 gtcctggcca gaggtccatc tcgctgagat acgaagggag ggtgtaccat tacaggatca 601 acactgcttc tgatggcaag ctctacgtct cctccgagag ccgcttcaac accctggccg 661 agttggttca tcatcattca acggtggccg acgggctcat caccacgctc cattatccag 721 ccccaaagcg caacaagccc actgtctatg gtgtgtcccc caactacgac aagtgggaga 781 tggaacgcac ggacatcacc atgaagcaca agctgggcgg gggccagtac ggggaggtgt 841 acgagggcgt gtggaagaaa tacagcctga cggtggccgt gaagaccttg aaggaggaca 901 ccatggaggt ggaagagttc ttgaaagaag ctgcagtcat gaaagagatc aaacacccta 961 acctggtgca gctccttggg gtctgcaccc gggagccccc gttctatatc atcattgagt 1021 tcatgaccta cgggaacctc ctggactacc tgagggagtg caaccggcag gaggtgaacg 1081 ccgtggtgct gctgtacatg gccactcaga tctcgtcagc catggagtac ctggagaaga 1141 aaaacttcat ccacagagat cttgctgccc gaaactgcct ggtaggggag aaccacttgg 1201 tgaaggtagc tgattttggc ctgagcaggt tgatgacagg ggacacctac acagcccatg 1261 ctggagccaa gttccccatc aaatggactg cacccgagag cctggcctac aacaagttct 1321 ccatcaagtc cgacgtctgg gcatttggag tattgctttg ggaaattgct acctatggca 1381 tgtcccctta cccgggaatt gacctgtccc aggtgtatga gctgctagag aaggactacc 1441 gcatggagcg cccagaaggc tgcccagaga aggtctatga actcatgcga gcatgttggc 1501 agtggaatcc ctctgaccgg ccctcctttg ctgaaatcca ccaagccttt gaaacaatgt 1561 tccaggaatc cagtatctca gacgaagtgg aaaaggagct ggggaaacaa ggcgtccgtg 1621 gggctgtgag taccttgctg caggccccag agctgcccac caagacgagg acctccagga 1681 gagctgcaga gcacagagac accactgacg tgcctgagat gcctcactcc aagggccagg 1741 gagagagcga tcctctggac catgagcctg ccgtgtctcc attgctccct cgaaaagagc 1801 gaggtccccc ggagggcggc ctgaatgaag atgagcgcct tctccccaaa gacaaaaaga 1861 ccaacttgtt cagcgccttg atcaagaaga agaagaagac agccccaacc cctcccaaac 1921 gcagcagctc cttccgggag atggacggcc agccggagcg cagaggggcc ggcgaggaag 1981 agggccgaga catcagcaac ggggcactgg ctttcacccc cttggacaca gctgacccag 2041 ccaagtcccc aaagcccagc aatggggctg gggtccccaa tggagccctc cgggagtccg 2101 ggggctcagg cttccggtct ccccacctgt ggaagaagtc cagcacgctg accagcagcc 2161 gcctagccac cggcgaggag gagggcggtg gcagctccag caagcgcttc ctgcgctctt 2221 gctccgcctc ctgcgttccc catggggcca aggacacgga gtggaggtca gtcacgctgc 2281 ctcgggactt gcagtccacg ggaagacagt ttgactcgtc cacatttgga gggcacaaaa 2341 gtgagaagcc ggctctgcct cggaagaggg caggggagaa caggtctgac caggtgaccc 2401 gaggcacagt aacgcctccc cccaggctgg tgaaaaagaa tgaggaagct gctgatgagg 2461 tcttcaaaga catcatggag tccagcccgg gctccagccc gcccaacctg actccaaaac 2521 ccctccggcg gcaggtcacc gtggcccctg cctcgggcct cccccacaag gaagaagctg 2581 gaaagggcag tgccttaggg acccctgctg cagctgagcc agtgaccccc accagcaaag 2641 caggctcagg tgcaccaggg ggcaccagca agggccccgc cgaggagtcc agagtgagga 2701 ggcacaagca ctcctctgag tcgccaggga gggacaaggg gaaattgtcc aggctcaaac 2761 ctgccccgcc gcccccacca gcagcctctg cagggaaggc tggaggaaag ccctcgcaga 2821 gcccgagcca ggaggcggcc ggggaggcag tcctgggcgc aaagacaaaa gccacgagtc 2881 tggttgatgc tgtgaacagt gacgctgcca agcccagcca gccgggagag ggcctcaaaa 2941 agcccgtgct cccggccact ccaaagccac agtccgccaa gccgtcgggg acccccatca 3001 gcccagcccc cgttccctcc acgttgccat cagcatcctc ggccctggca ggggaccagc 3061 cgtcTTCCAC CGCCTTCATC CCTCTCATAT CAACCCGAGT GTCTCTTCGG AAAACCCGCC 3121 AGCCTCCAGA GCGGATCGCC AGCGGCGCCA TCACCAAGGG CGTGGTCCTG GACAGCACCG 3181 AGGCGCTGTG CCTCGCCATC TCTAGGAACT CCGAGCAGAT GGCCAGCCAC AGCGCAGTGC 3241 TGGAGGCCGG CAAAAACCTC TACACGTTCT GCGTGAGCTA TGTGGATTCC ATCCAGCAAA 3301 TGAGGAACAA GTTTGCCTTC CGAGAGGCCA TCAACAAACT GGAGAATAAT CTCCGGGAGC 3361 TTCAGATCTG CCCGGCGACA GCAGGCAGTG GTCCGGCGGC CACTCAGGAC TTCAGCAAGC 3421 TCCTCAGTTC GGTGAAGGAA ATCAGTGACA TAGTGCAGAG GTAGGACCCA GCTTTCTTGT 3481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3601 GAAAGGACGA CGATTCCTAA CACCTTCTAA CGTCACGCGT TAAGTCgaca atcaacctct 3661 ggattacaaa atttgtgaaa gatt