Construct: ORF TRCN0000489612

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020728.1_s317c1
DNA Barcode:
CGATTCCTAACACCTTCTAACGTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ABL1 (25)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489612
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25 ABL1 ABL proto-oncogene 1, non-r... NM_005157.3:c.944C>T 99.9% 100% 3324A>G
2 human 25 ABL1 ABL proto-oncogene 1, non-r... NM_005157.6 99.9% 99.9% 944C>T;3324A>G
3 human 25 ABL1 ABL proto-oncogene 1, non-r... NM_007313.2 97.6% 96.4% (many diffs)
4 mouse 11350 Abl1 c-abl oncogene 1, non-recep... NM_009594.4 84.2% 87.6% (many diffs)
5 mouse 11350 Abl1 c-abl oncogene 1, non-recep... NM_001283045.1 83% 84.7% (many diffs)
6 mouse 11350 Abl1 c-abl oncogene 1, non-recep... NM_001283046.1 82.9% 85.3% (many diffs)
7 mouse 11350 Abl1 c-abl oncogene 1, non-recep... NM_001112703.2 82.4% 84.6% (many diffs)
8 mouse 11350 Abl1 c-abl oncogene 1, non-recep... NM_001283047.1 65.5% 66.9% (many diffs)
9 mouse 11350 Abl1 c-abl oncogene 1, non-recep... XM_006497621.3 44.3% 45.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3462
ORF length:
3390
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgttggag atctgcctga agctggtggg ctgcaaatcc aagaaggggc
121tgtcctcgtc ctccagctgt tatctggaag aagcccttca gcggccagta gcatctgact
181ttgagcctca gggtctgagt gaagccgctc gttggaactc caaggaaaac cttctcgctg
241gacccagtga aaatgacccc aaccttttcg ttgcactgta tgattttgtg gccagtggag
301ataacactct aagcataact aaaggtgaaa agctccgggt cttaggctat aatcacaatg
361gggaatggtg tgaagcccaa accaaaaatg gccaaggctg ggtcccaagc aactacatca
421cgccagtcaa cagtctggag aaacactcct ggtaccatgg gcctgtgtcc cgcaatgccg
481ctgagtatct gctgagcagc gggatcaatg gcagcttctt ggtgcgtgag agtgagagca
541gtcctggcca gaggtccatc tcgctgagat acgaagggag ggtgtaccat tacaggatca
601acactgcttc tgatggcaag ctctacgtct cctccgagag ccgcttcaac accctggccg
661agttggttca tcatcattca acggtggccg acgggctcat caccacgctc cattatccag
721ccccaaagcg caacaagccc actgtctatg gtgtgtcccc caactacgac aagtgggaga
781tggaacgcac ggacatcacc atgaagcaca agctgggcgg gggccagtac ggggaggtgt
841acgagggcgt gtggaagaaa tacagcctga cggtggccgt gaagaccttg aaggaggaca
901ccatggaggt ggaagagttc ttgaaagaag ctgcagtcat gaaagagatc aaacacccta
961acctggtgca gctccttggg gtctgcaccc gggagccccc gttctatatc atcattgagt
1021tcatgaccta cgggaacctc ctggactacc tgagggagtg caaccggcag gaggtgaacg
1081ccgtggtgct gctgtacatg gccactcaga tctcgtcagc catggagtac ctggagaaga
1141aaaacttcat ccacagagat cttgctgccc gaaactgcct ggtaggggag aaccacttgg
1201tgaaggtagc tgattttggc ctgagcaggt tgatgacagg ggacacctac acagcccatg
1261ctggagccaa gttccccatc aaatggactg cacccgagag cctggcctac aacaagttct
1321ccatcaagtc cgacgtctgg gcatttggag tattgctttg ggaaattgct acctatggca
1381tgtcccctta cccgggaatt gacctgtccc aggtgtatga gctgctagag aaggactacc
1441gcatggagcg cccagaaggc tgcccagaga aggtctatga actcatgcga gcatgttggc
1501agtggaatcc ctctgaccgg ccctcctttg ctgaaatcca ccaagccttt gaaacaatgt
1561tccaggaatc cagtatctca gacgaagtgg aaaaggagct ggggaaacaa ggcgtccgtg
1621gggctgtgag taccttgctg caggccccag agctgcccac caagacgagg acctccagga
1681gagctgcaga gcacagagac accactgacg tgcctgagat gcctcactcc aagggccagg
1741gagagagcga tcctctggac catgagcctg ccgtgtctcc attgctccct cgaaaagagc
1801gaggtccccc ggagggcggc ctgaatgaag atgagcgcct tctccccaaa gacaaaaaga
1861ccaacttgtt cagcgccttg atcaagaaga agaagaagac agccccaacc cctcccaaac
1921gcagcagctc cttccgggag atggacggcc agccggagcg cagaggggcc ggcgaggaag
1981agggccgaga catcagcaac ggggcactgg ctttcacccc cttggacaca gctgacccag
2041ccaagtcccc aaagcccagc aatggggctg gggtccccaa tggagccctc cgggagtccg
2101ggggctcagg cttccggtct ccccacctgt ggaagaagtc cagcacgctg accagcagcc
2161gcctagccac cggcgaggag gagggcggtg gcagctccag caagcgcttc ctgcgctctt
2221gctccgcctc ctgcgttccc catggggcca aggacacgga gtggaggtca gtcacgctgc
2281ctcgggactt gcagtccacg ggaagacagt ttgactcgtc cacatttgga gggcacaaaa
2341gtgagaagcc ggctctgcct cggaagaggg caggggagaa caggtctgac caggtgaccc
2401gaggcacagt aacgcctccc cccaggctgg tgaaaaagaa tgaggaagct gctgatgagg
2461tcttcaaaga catcatggag tccagcccgg gctccagccc gcccaacctg actccaaaac
2521ccctccggcg gcaggtcacc gtggcccctg cctcgggcct cccccacaag gaagaagctg
2581gaaagggcag tgccttaggg acccctgctg cagctgagcc agtgaccccc accagcaaag
2641caggctcagg tgcaccaggg ggcaccagca agggccccgc cgaggagtcc agagtgagga
2701ggcacaagca ctcctctgag tcgccaggga gggacaaggg gaaattgtcc aggctcaaac
2761ctgccccgcc gcccccacca gcagcctctg cagggaaggc tggaggaaag ccctcgcaga
2821gcccgagcca ggaggcggcc ggggaggcag tcctgggcgc aaagacaaaa gccacgagtc
2881tggttgatgc tgtgaacagt gacgctgcca agcccagcca gccgggagag ggcctcaaaa
2941agcccgtgct cccggccact ccaaagccac agtccgccaa gccgtcgggg acccccatca
3001gcccagcccc cgttccctcc acgttgccat cagcatcctc ggccctggca ggggaccagc
3061cgtcTTCCAC CGCCTTCATC CCTCTCATAT CAACCCGAGT GTCTCTTCGG AAAACCCGCC
3121AGCCTCCAGA GCGGATCGCC AGCGGCGCCA TCACCAAGGG CGTGGTCCTG GACAGCACCG
3181AGGCGCTGTG CCTCGCCATC TCTAGGAACT CCGAGCAGAT GGCCAGCCAC AGCGCAGTGC
3241TGGAGGCCGG CAAAAACCTC TACACGTTCT GCGTGAGCTA TGTGGATTCC ATCCAGCAAA
3301TGAGGAACAA GTTTGCCTTC CGAGAGGCCA TCAACAAACT GGAGAATAAT CTCCGGGAGC
3361TTCAGATCTG CCCGGCGACA GCAGGCAGTG GTCCGGCGGC CACTCAGGAC TTCAGCAAGC
3421TCCTCAGTTC GGTGAAGGAA ATCAGTGACA TAGTGCAGAG GTAGGACCCA GCTTTCTTGT
3481ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
3541AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
3601GAAAGGACGA CGATTCCTAA CACCTTCTAA CGTCACGCGT TAAGTCgaca atcaacctct
3661ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)