Construct: ORF TRCN0000489616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020327.1_s317c1
- DNA Barcode:
- GAGTGCTCGCGCCTTACTATGATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK38 (11329)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489616
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11329 | STK38 | serine/threonine kinase 38 | NM_001305102.2 | 100% | 100% | |
| 2 | human | 11329 | STK38 | serine/threonine kinase 38 | NM_007271.4 | 100% | 100% | |
| 3 | human | 11329 | STK38 | serine/threonine kinase 38 | XM_006714988.4 | 100% | 100% | |
| 4 | human | 11329 | STK38 | serine/threonine kinase 38 | XM_006714989.4 | 100% | 100% | |
| 5 | human | 11329 | STK38 | serine/threonine kinase 38 | XM_017010226.1 | 93% | 90.8% | (many diffs) |
| 6 | human | 11329 | STK38 | serine/threonine kinase 38 | XR_001743146.1 | 35.6% | 1_292del;1126_1127ins118;1570_3467del | |
| 7 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | NM_134115.2 | 92.8% | 98.7% | (many diffs) |
| 8 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_011246232.2 | 82.2% | 87.7% | (many diffs) |
| 9 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_011246233.2 | 82.2% | 87.7% | (many diffs) |
| 10 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_006523386.2 | 78.7% | 83.7% | (many diffs) |
| 11 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_006523385.2 | 78.6% | 83.6% | (many diffs) |
| 12 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_017317194.1 | 57.9% | 61.5% | (many diffs) |
| 13 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_017317192.1 | 44.3% | 41.6% | (many diffs) |
| 14 | mouse | 106504 | Stk38 | serine/threonine kinase 38 | XM_017317193.1 | 34.4% | 32.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1467
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcaatg acaggctcaa caccttgctc atccatgagt aaccacacaa 121 aggaaagggt gacaatgacc aaagtgacac tggagaattt ttatagcaac cttatcgctc 181 aacatgaaga acgagaaatg agacaaaaga agttagaaaa ggtgatggaa gaagaaggcc 241 taaaagatga ggagaaacga ctccggagat cagcacatgc tcggaaggaa acagagtttc 301 ttcgtttgaa gagaacaaga cttggattgg aagattttga gtccttaaaa gtaataggca 361 gaggagcatt tggtgaggta cggcttgttc agaagaaaga tacgggacat gtgtatgcaa 421 tgaaaatact ccgtaaagca gatatgcttg aaaaagagca ggttggccac attcgtgcgg 481 agcgtgacat tctagtggag gcagacagtt tgtgggttgt gaaaatgttc tatagttttc 541 aggataagct aaacctctac ctaatcatgg agttcctgcc tggaggggac atgatgacct 601 tgttgatgaa aaaagacact ctgacagaag aggagactca gttttatata gcagaaacag 661 tattagccat agactctatt caccaacttg gattcatcca cagagacatc aaaccagaca 721 accttctttt ggacagcaag ggccatgtga aactttctga ctttggtctt tgcacaggac 781 tgaaaaaagc acataggaca gaattttata ggaatctgaa ccacagcctc cccagtgatt 841 tcactttcca gaacatgaat tccaaaagga aagcagaaac ctggaaaaga aatagacgtc 901 agctagcctt ctccacagta ggcactcctg actacattgc tcctgaggtg ttcatgcaga 961 ccgggtacaa caagctctgt gattggtggt cgcttggggt gatcatgtat gagatgctca 1021 tcggctaccc acctttctgt tctgagaccc ctcaagagac atataagaag gtgatgaact 1081 ggaaagaaac tttgactttt cctccagaag ttcccatctc tgagaaagcc aaggatctaa 1141 ttttGAGGTT CTGCTGTGAA TGGGAACATA GAATTGGAGC TCCTGGAGTT GAGGAAATAA 1201 AAAGTAACTC TTTTTTTGAA GGCGTTGACT GGGAACATAT CAGAGAGAGA CCTGCTGCAA 1261 TATCTATTGA AATCAAAAGC ATTGATGATA CCTCAAACTT CGATGAGTTT CCAGAATCTG 1321 ATATTCTTAA GCCAACAGTG GCCACAAGTA ATCATCCTGA GACTGACTAC AAGAACAAAG 1381 ACTGGGTCTT CATCAATTAC ACGTACAAGC GCTTTGAGGG CCTGACTGCA AGGGGGGCAA 1441 TACCTTCCTA CATGAAAGCA GCAAAATGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGTG 1621 CTCGCGCCTT ACTATGATCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt