Construct: ORF TRCN0000489619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020066.1_s317c1
- DNA Barcode:
- AATGGCTCTCTTGTCCGGCATGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NUMB (8650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005745.1 | 94.8% | 0_1ins96;1776_1777insG | |
| 2 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005744.1 | 93.1% | 0_1ins96;202_234del;1809_1810insG | |
| 3 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001320114.1 | 93.1% | 0_1ins96;202_234del;1809_1810insG | |
| 4 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_003744.5 | 88% | 0_1ins96;1063_1206del;1920_1921insG | |
| 5 | human | 8650 | NUMB | NUMB endocytic adaptor protein | NM_001005743.1 | 86.6% | (many diffs) | |
| 6 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272056.1 | 84.3% | (many diffs) | |
| 7 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_010949.2 | 82.9% | (many diffs) | |
| 8 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515575.2 | 80.3% | (many diffs) | |
| 9 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515574.2 | 79% | (many diffs) | |
| 10 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001272055.1 | 78.4% | (many diffs) | |
| 11 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515578.1 | 78.4% | (many diffs) | |
| 12 | mouse | 18222 | Numb | numb homolog (Drosophila) | NM_001136075.2 | 77.1% | (many diffs) | |
| 13 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515576.1 | 77.1% | (many diffs) | |
| 14 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515577.2 | 77.1% | (many diffs) | |
| 15 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_011244012.2 | 77.1% | (many diffs) | |
| 16 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515573.2 | 75.1% | (many diffs) | |
| 17 | mouse | 18222 | Numb | numb homolog (Drosophila) | XM_006515572.2 | 74% | (many diffs) | |
| 18 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073563.1 | 46.6% | (many diffs) | |
| 19 | mouse | 18222 | Numb | numb homolog (Drosophila) | NR_073564.1 | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 77
- ORF length:
- 12
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccca 61 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg 121 cttttttata atgccaactt tgtacaaaaa agcaggcacc atgaacaaat tacggcaaag 181 ttttaggaga aagaaggatg tttatgttcc agaggccagt cgtccacatc agtggcagac 241 agatgaagaa ggcgttcgca ccggaaaatg tagcttcccg gttaagtacc ttggccatgt 301 agaagttgat gaatcaagag gaatgcacat ctgtgaagat gctgtaaaaa gattgaaagc 361 tactggaaag aaagcagtta aagcagttct gtgggtctca gcagatggac tcagagttgt 421 ggatgaaaaa actaaggacc tcatagttga ccagacgata gagaaagttt ctttctgtgc 481 cccagacagg aactttgata gagccttttc ttacatatgc cgtgatggca ccactcgtcg 541 ctggatctgt cactgcttca tggctgtcaa ggacacaggt gaaaggttga gccatgcagt 601 aggctgtgct tttgcagcct gtttagagcg caagcagaag cgggagaagg aatgtggagt 661 gactgctact tttgatgcta gtcggaccac ttttacaaga gaaggatcat tccgtgtcac 721 aacagccact gaacaagcag aaagagagga gatcatgaaa caaatgcaag atgccaagaa 781 agctgaaaca gataagatag tcgttggttc atcagttgcc cctggcaaca ctgccccatc 841 cccatcctct cccacctctc ctacttctga tgccacgacc tctctggaga tgaacaatcc 901 tcatgccatc ccacgccggc atgctccaat tgaacagctt gctcgccaag gctctttccg 961 aggttttcct gctcttagcc agaagatgtc accctttaaa cgccaactat ccctacgcat 1021 caatgagttg ccttccacta tgcagaggaa gactgatttc cccattaaaa atgcagtgcc 1081 agaagtagaa ggggaggcag agagcatcag ctccctgtgc tcacagatca ccaatgcctt 1141 cagcacacct gaggacccct tctcatctgc tccgatgacc aaaccagtga cagtggtggc 1201 accacaatct cctaccttcc aagggaccga gtggggtcaa tcttctggtg ctgcctctcc 1261 aggtctcttc caggccggtc atagacgtac tccctctgag gccgaccgat ggttagaaga 1321 ggtgtctaag agcgtccggg ctcagcagcc ccaggcctca gctgctcctc tgcagccagt 1381 tctccagcct cctccaccca ctgccatctc ccagccagca tcacctttcc aagggaatgc 1441 attcctcacc tctcagcctg tgccagtggg tgtggtccca gccctgcaac cagcctttgt 1501 ccctgcccag tcctatcctg tggccaatgg aatgccctat ccagccccta atgtgcctgt 1561 ggtgggcatc actcccTCCC AGATGGTGGC CAACGTATTT GGCACTGCAG GCCACCCTCA 1621 GGCTGCCCAT CCCCATCAGT CACCCAGCCT GGTCAGGCAG CAGACATTCC CTCACTACGA 1681 GGCAAGCAGT GCTACCACCA GTCCCTTCTT TAAGCCTCCT GCTCAGCACC TCAACGGTTC 1741 TGCAGCTTTC AATGGTGTAG ATGATGGCAG GTTGGCCTCA GCAGACAGGC ATACAGAGGT 1801 TCCTACAGGC ACCTGCCCAG TGGATCCTTT TGAAGCCCAG TGGGCTGCAT TAGAAAATAA 1861 GTCCAAGCAG CGTACTAATC CCTCCCCTAC CAACCCTTTC TCCAGTGACT TACAGAAGAC 1921 GTTTGAAATT GAACTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 1981 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 2041 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATGGCTC TCTTGTCCGG 2101 CATGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt