Construct: ORF TRCN0000489626
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019573.1_s317c1
- DNA Barcode:
- TTGCTAGTATCTTCGAGTTCCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRHR1 (1394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489626
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_004382.5 | 99.9% | 99.7% | 1245_1246insG |
| 2 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001145148.2 | 96.5% | 96.3% | 1063_1064ins42;1203_1204insG |
| 3 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001145146.2 | 93.3% | 93.2% | 433_519del;1332_1333insG |
| 4 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001145147.2 | 90.2% | 89.9% | 121_122ins120;1125_1126insG |
| 5 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001303020.1 | 75.6% | 73.5% | 0_1ins217;24_25ins86;942_943insG |
| 6 | human | 104909134 | LINC02210-CRHR1 | LINC02210-CRHR1 readthrough | NM_001303016.1 | 62.2% | 50.3% | (many diffs) |
| 7 | human | 104909134 | LINC02210-CRHR1 | LINC02210-CRHR1 readthrough | NM_001256299.3 | 57.7% | 57.6% | 0_1ins525;720_721insG |
| 8 | human | 1394 | CRHR1 | corticotropin releasing hor... | NM_001303018.2 | 57.7% | 57.6% | 0_1ins525;720_721insG |
| 9 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | NM_007762.5 | 91% | 97.3% | (many diffs) |
| 10 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | NM_001313928.1 | 82.1% | 87.5% | (many diffs) |
| 11 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XM_017314246.1 | 75.8% | 71.9% | (many diffs) |
| 12 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XM_017314247.1 | 75.8% | 71.9% | (many diffs) |
| 13 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | NM_001313929.1 | 45.9% | 50% | (many diffs) |
| 14 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XR_001779871.1 | 40.7% | (many diffs) | |
| 15 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XR_001779869.1 | 34% | (many diffs) | |
| 16 | mouse | 12921 | Crhr1 | corticotropin releasing hor... | XR_001779870.1 | 32.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1323
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggga gggcacccgc agctccgtct cgtcaaggcc cttctccttc 121 tggggctgaa ccccgtctct gcctccctcc aggaccagca ctgcgagagc ctgtccctgg 181 ccagcaacat ctcaggactg cagtgcaacg catccgtgga cctcattggc acctgctggc 241 cccgcagccc tgcggggcag ctagtggttc ggccctgccc tgcctttttc tatggtgtcc 301 gctacaatac cacaaacaat ggctaccggg agtgcctggc caatggcagc tgggccgccc 361 gcgtgaatta ctccgagtgc caggagatcc tcaatgagga gaaaaaaagc aaggtgcact 421 accatgtcgc agtcatcatc aactacctgg gccactgtat ctccctggtg gccctcctgg 481 tggcctttgt cctctttctg cggctcagga gcatccggtg cctgcgaaac atcatccact 541 ggaacctcat ctccgccttc atcctgcgca acgccacctg gttcgtggtc cagctaacca 601 tgagccccga ggtccaccag agcaacgtgg gctggtgcag gttggtgaca gccgcctaca 661 actacttcca tgtgaccaac ttcttctgga tgttcggcga gggctgctac ctgcacacag 721 ccatcgtgct cacctactcc actgaccggc tgcgcaaatg gatgttcatc tgcattggct 781 ggggtgtgcc cttccccatc attgtggcct gggccattgg gaagctgtac tacgacaatg 841 agaagtgctg gtttggcaaa aggcctgggg tgtacaccga ctacatctac cagggcccca 901 tgatcctggt cctgctgatc aatttcatct tccttttcaa catcgtccgc atcctcatga 961 ccaagctccg ggCATCCACC ACGTCTGAGA CCATTCAGTA CAGGAAGGCT GTGAAAGCCA 1021 CTCTGGTGCT GCTGCCCCTC CTGGGCATCA CCTACATGCT GTTCTTCGTC AATCCCGGGG 1081 AGGATGAGGT CTCCCGGGTC GTCTTCATCT ACTTCAACTC CTTCCTGGAA TCCTTCCAGG 1141 GCTTCTTTGT GTCTGTGTTC TACTGTTTCC TCAATAGTGA GGTCCGTTCT GCCATCCGGA 1201 AGAGGTGGCA CCGGTGGCAG GACAAGCACT CGATCCGTGC CCGAGTGGCC CGTGCCATGT 1261 CCATCCCCAC CTCCCCAACC CGTGTCAGCT TTCACAGCAT CAAGCAGTCC ACAGCAGTCG 1321 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGATTGCT AGTATCTTCG AGTTCCGGGA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt