Construct: ORF TRCN0000489649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020368.1_s317c1
- DNA Barcode:
- CTCCGAAGCTAATTCATGGTAGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AXL (558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489649
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_021913.5 | 99.9% | 99.8% | 1546C>T;2291A>G |
| 2 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_001699.6 | 98.9% | 98.8% | 1284_1285ins27;1519C>T;2264A>G |
| 3 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_001278599.1 | 69.9% | 69.9% | 0_1ins804;742C>T;1487A>G |
| 4 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | NM_009465.4 | 84.9% | 87.4% | (many diffs) |
| 5 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539989.3 | 84.9% | 87.4% | (many diffs) |
| 6 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | NM_001190974.1 | 84% | 86.8% | (many diffs) |
| 7 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539991.3 | 84% | 86.8% | (many diffs) |
| 8 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | NM_001190975.1 | 75.7% | 78.5% | (many diffs) |
| 9 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539992.2 | 75.7% | 78.5% | (many diffs) |
| 10 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539993.2 | 74.9% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2754
- ORF length:
- 2682
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgtgg cggtgcccca ggatgggcag ggtcccgctg gcctggtgct 121 tggcgctgtg cggctgggcg tgcatggccc ccaggggcac gcaggctgaa gaaagtccct 181 tcgtgggcaa cccagggaat atcacaggtg cccggggact cacgggcacc cttcggtgtc 241 agctccaggt tcagggagag ccccccgagg tacattggct tcgggatgga cagatcctgg 301 agctcgcgga cagcacccag acccaggtgc ccctgggtga ggatgaacag gatgactgga 361 tagtggtcag ccagctcaga atcacctccc tgcagctttc cgacacggga cagtaccagt 421 gtttggtgtt tctgggacat cagaccttcg tgtcccagcc tggctatgtt gggctggagg 481 gcttgcctta cttcctggag gagcccgaag acaggactgt ggccgccaac acccccttca 541 acctgagctg ccaagctcag ggacccccag agcccgtgga cctactctgg ctccaggatg 601 ctgtccccct ggccacggct ccaggtcacg gcccccagcg cagcctgcat gttccagggc 661 tgaacaagac atcctctttc tcctgcgaag cccataacgc caagggggtc accacatccc 721 gcacagccac catcacagtg ctcccccagc agccccgtaa cctccacctg gtctcccgcc 781 aacccacgga gctggaggtg gcttggactc caggcctgag cggcatctac cccctgaccc 841 actgcaccct gcaggctgtg ctgtcagacg atgggatggg catccaggcg ggagaaccag 901 accccccaga ggagcccctc acctcgcaag catccgtgcc cccccatcag cttcggctag 961 gcagcctcca tcctcacacc ccttatcaca tccgcgtggc atgcaccagc agccagggcc 1021 cctcatcctg gacccactgg cttcctgtgg agacgccgga gggagtgccc ctgggccccc 1081 ctgagaacat tagtgctacg cggaatggga gccaggcctt cgtgcattgg caagagcccc 1141 gggcgcccct gcagggtacc ctgttagggt accggctggc gtatcaaggc caggacaccc 1201 cagaggtgct aatggacata gggctaaggc aagaggtgac cctggagctg cagggggacg 1261 ggtctgtgtc caatctgaca gtgtgtgtgg cagcctacac tgctgctggg gatggaccct 1321 ggagcctccc agtacccctg gaggcctggc gcccagggca agcacagcca gtccaccagc 1381 tggtgaagga accttcaact cctgccttct cgtggccctg gtggtatgta ctgctaggag 1441 cagtcgtggc cgctgcctgt gtcctcatct tggctctctt ccttgtccac cggcgaaaga 1501 aggagacccg ttatggagaa gtgtttgaac caacagtgga aagaggtgaa ctggtagtca 1561 ggtaccgcgt gcgcaagtcc tacagtcgtc ggaccactga agctaccttg aacagcttgg 1621 gcatcagtga agagctgaag gagaagctgc gggatgtgat ggtggaccgg cacaaggtgg 1681 ccctggggaa gactctggga gagggagagt ttggagctgt gatggaaggc cagctcaacc 1741 aggacgactc catcctcaag gtggctgtga agacgatgaa gattgccatc tgcacgaggt 1801 cagagctgga ggatttcctg agtgaagcgg tctgcatgaa ggaatttgac catcccaacg 1861 tcatgaggct catcggtgtc tgtttccagg gttctgaacg agagagcttc ccagcacctg 1921 tggtcatctt acctttcatg aaacatggag acctacacag cttcctcctc tattcccggc 1981 tcggggacca gccagtgtac ctgcccactc agatgctagt gaagttcatg gcagacatcg 2041 ccagtggcat ggagtatctg agtaccaaga gattcataca ccgggacctg gcggccagga 2101 actgcatgct gaatgagaac atgtccgtgt gtgtggcgga cttcgggctc tccaagaaga 2161 tctacaatgg ggactactac cgccagggac gtatcgccaa gatgccagtc aagtggattg 2221 ccattgagag tctagctgac cgtgtctaca ccagcaagag cgatgtgtgg tccttcgggg 2281 tgacaatgtg ggagattgcc acaagaggcc aaaccccata tccgggcgtg gagaacagcg 2341 agatttatga ctatctgcgc cggggaaatc gcctgaagca gccTGCGGAC TGTCTGGATG 2401 GACTGTATGC CTTGATGTCG CGGTGCTGGG AGCTAAATCC CCAGGACCGG CCAAGTTTTA 2461 CAGAGCTGCG GGAAGATTTG GAGAACACAC TGAAGGCCTT GCCTCCTGCC CAGGAGCCTG 2521 ACGAAATCCT CTATGTCAAC ATGGATGAGG GTGGAGGTTA TCCTGAACCC CCTGGAGCTG 2581 CAGGAGGAGC TGACCCCCCA ACCCAGCCAG ACCCTAAGGA TTCCTGTAGC TGCCTCACTG 2641 CGGCTGAGGT CCATCCTGCT GGACGCTATG TCCTCTGCCC TTCCACAACC CCTAGCCCCG 2701 CTCAGCCTGC TGATAGGGGC TCCCCAGCAG CCCCAGGGCA GGAGGATGGT GCCTAGGACC 2761 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2821 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2881 ATATATCTTG TGGAAAGGAC GACTCCGAAG CTAATTCATG GTAGCAACGC GTTAAGTCga 2941 caatcaacct ctggattaca aaatttgtga aagatt